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Species: Other
Genetic Insert: EGFP with early endosome-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174454 Copy
Species: Mus musculus
Genetic Insert: JMJD3
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pCS2+ NLSMT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18003914
Comments: Full length mouse JMJD3 with a C-terminal FLAG was PCR-amplified from MGC clone BC075632 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.
Proper citation: RRID:Addgene_17440 Copy
Vector Backbone Description: Backbone Marker:Philippa Marrack Lab; Backbone Size:6063; Vector Backbone:MSCV-IRES-Thy1.1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16873067
Comments: Recombination sites were added to MSCV-IRES-Thy1.1 for subcloning from pENTR vectors.
Proper citation: RRID:Addgene_17442 Copy
Species: Homo sapiens
Genetic Insert: FOXP3
Vector Backbone Description: Backbone Size:6063; Vector Backbone:MSCV-IRES-Thy1.1 DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16873067
Proper citation: RRID:Addgene_17443 Copy
Species: Homo sapiens
Genetic Insert: small heat shock protein27
Vector Backbone Description: Backbone Marker:BD Biosciences Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17597071
Proper citation: RRID:Addgene_17444 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:8591; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17446 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:8068; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17447 Copy
Species: Other
Genetic Insert: protein A - hia5
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35396487
Comments: The depositing laboratory recommends Addgene Plasmid 222303: prhaBAD-pA-Hia5, an improved version of this plasmid.
Protocol for purification and expression can be found at: dx.doi.org/10.17504/protocols.io.bv82n9ye
Application for DiMeLo-seq is described in: doi.org/10.1101/2021.07.06.451383
Protocol was adapted and modified from: doi.org/10.1126/science.aaz1646
Proper citation: RRID:Addgene_174372 Copy
Genetic Insert: TEV Protease
Vector Backbone Description: Vector Backbone:pB-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37053554
Proper citation: RRID:Addgene_174377 Copy
Genetic Insert: FLPo recombinase
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38212587
Proper citation: RRID:Addgene_174378 Copy
Species: Candida auris
Genetic Insert: NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
Vector Backbone Description: Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174405 Copy
Species: Candida auris
Genetic Insert: C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
Vector Backbone Description: Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174409 Copy
Species: Streptomyces tendae
Genetic Insert: nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
Vector Backbone Description: Backbone Size:3516; Vector Backbone:pCm No carrier; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35101207
Proper citation: RRID:Addgene_174361 Copy
Species: Salmonella enterica ser. Typhimurium
Genetic Insert: sarA
Vector Backbone Description: Vector Backbone:pWSK129; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31901521
Proper citation: RRID:Addgene_174401 Copy
Vector Backbone Description: Backbone Size:9617; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17452 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3313; Vector Backbone:pENTR1A; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17453 Copy
Vector Backbone Description: Backbone Size:10319; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: Please note that there are several mismatches between depositor's sequence and Addgene's quality control sequence. These mismatches are in the backbone regions only and do not affect any of the features.
Proper citation: RRID:Addgene_17454 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2595; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression, Plant Expression, modified Gateway Entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18211686
Comments: Part of a modified Gateway Entry vector system for generating epitope tagged constructs. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a N-terminal 6x MYC tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.
The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).
GenBank accession number: EU334818
Proper citation: RRID:Addgene_17457 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:5728; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17449 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN2
Vector Backbone Description: Vector Backbone:pnEA-vH; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34237139
Proper citation: RRID:Addgene_174497 Copy
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