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Species: Rattus norvegicus
Genetic Insert: G protein alpha 0
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7261; Vector Backbone:pd12.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15078878
Comments: The CFP was introduced between 92nd and 93rd amino acid of alpha-0
Proper citation: RRID:Addgene_36312 Copy
Genetic Insert: shRNA of scramble
Vector Backbone Description: Backbone Marker:Biosettia; Backbone Size:10275; Vector Backbone:pLV-H1TetO-RFP-Puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22645348
Comments: The plasmid backbone is TetO-H1-shRNA-EF1a-TetR-IRES-RFP-Puro. It is a all-in-one inducible shRNA knockdown lentiviral plasmid.
The paper associated with this plasmid is "Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling"
Proper citation: RRID:Addgene_36311 Copy
Species: Synthetic
Genetic Insert: mito-ZifCY1 genetically encoded Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19363034
Comments: First generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from mammalian Zif268. mito-ZifCY1 contains 4 tandem repeats of the first 29 aa of human cox subunit 8a (MSVLTPLLLRGLTGSARRLPVPRAKIHSL). HindIII restriction sites are at the 5' and 3' ends of the mitochondrial targeting sequence.
Proper citation: RRID:Addgene_36317 Copy
Species: Synthetic
Genetic Insert: ZifCY2 genetically encoded Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19363034
Comments: First generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from mammalian Zif268. ZifCY2 is a variant of ZifCY1. The two cysteines (amino acids 237 and 242) in the Zn(II)-binding domain of ZifCY1 are mutated to histidines in ZifCY2.
Proper citation: RRID:Addgene_36316 Copy
Genetic Insert: pXylan
Vector Backbone Description: Vector Backbone:Biobrick family of vectors; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22123987
Comments: Note from depositor regarding growing this plasmid: W used DH10B, plated on LB/carb, and grew the plasmid up in TB medium plus 2% glycerol and 100 ug/mL carbenicillin. It's key to not grow the cells up too long, either on the plate or in the liquid medium, and probably best to harvest the cells in early stationary phase.
Proper citation: RRID:Addgene_36301 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36389 Copy
Species: Homo sapiens
Genetic Insert: ubiquitin specific peptidase 19
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21849505
Proper citation: RRID:Addgene_36307 Copy
Species: Homo sapiens
Genetic Insert: DNMT1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Comments: Addgene's quality control sequence varies slightly from the depositor's, but this plasmid should be functional.
Proper citation: RRID:Addgene_36383 Copy
Species: Homo sapiens
Genetic Insert: EED
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36386 Copy
Species: Homo sapiens
Genetic Insert: DNMT1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36384 Copy
Species: Mus musculus
Genetic Insert: mTERT
Vector Backbone Description: Backbone Marker:Bob Weinberg (Addgene Plasmid #1764); Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21732358
Proper citation: RRID:Addgene_36413 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Plasmid carrying monomeric (A206K) EGFP for N-terminal tagging
Proper citation: RRID:Addgene_36412 Copy
Species: Homo sapiens
Genetic Insert: active BCR-related
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17116687
Comments: This is the ABR isoform A. The R683A N795A double mutant lacks GTPase activity towards RacGTP. Construct: Young-jin Cho. Also see PMID 20962234.
Proper citation: RRID:Addgene_36417 Copy
Species: Homo sapiens
Genetic Insert: breakpoint cluster region protein isoform 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20962234
Comments: Also see PMID 17116687.
Proper citation: RRID:Addgene_36416 Copy
Species: Homo sapiens
Genetic Insert: active BCR-related
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17116687
Comments: Made by direct mutagenesis of EYFP-Abr using a Stratagene Quick Change kit. In Dbl, mutation of residues N673 and D674 completely abolished its activity as a GEF towards Rac. These residues are highly conserved among DH domains and are also conserved as residues N245/E246 in Abr. We therefore mutated these residues in Abr and introduced the mutant into the RAW 264.7 macrophage cell line. However, the construct’s expression did not cause morphological changes in these cells. constructed by Young-Jin Cho.
Proper citation: RRID:Addgene_36419 Copy
Species: Homo sapiens
Genetic Insert: breakpoint cluster region
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17116687
Comments: Made by mutagenesis directly on plasmid Bcr-ECFP using a Stratagene Quick Change kit. In Dbl, mutation of residues N673 and D674 completely abolished its activity as a GEF towards Rac. These residues are highly conserved among DH domains and are also conserved as residues N689/ E690 in Bcr. We therefore mutated these residues in Bcr and introduced the mutants into the RAW 264.7 macrophage cell line. However, the construct did not caused morphological changes in these cells. Constructed by Young-Jin Cho.
Proper citation: RRID:Addgene_36418 Copy
Species: Danio rerio
Genetic Insert: Hox D4a
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression, Riboprobe Synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9425136
Comments: Please note that Addgene's sequencing results indicate the Hox D4a insert in this plasmid has Q100H, A101T, G125C and R189W mutations when compared to GenBank entry NP_001119917.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication.
Proper citation: RRID:Addgene_36469 Copy
Species: Danio rerio
Genetic Insert: hoxa11b
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Riboprobe Synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18638469
Comments: Please note that Addgene's sequencing results indicate the Hox D13a insert in this plasmid has F63I, P75Q and M133I mutations when compared to GenBank entry AAI53653.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication.
Proper citation: RRID:Addgene_36467 Copy
Species: Homo sapiens
Genetic Insert: EHD1 EH domain
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_36459 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ARS501-LEU2
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pNRB8; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is for generating dmc1Δ::LEU2 alleles by single step transplacement. See the attached map for additional details.
Proper citation: RRID:Addgene_36455 Copy
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