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Species: Sperm Whale: Physeter macrocephalus
Genetic Insert: Myoglobin-127TAG
Vector Backbone Description: Vector Backbone:pSepT; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21868676
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid.
Proper citation: RRID:Addgene_34625 Copy
Species: Homo sapiens
Genetic Insert: PRMT7
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX(SN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15044439
Proper citation: RRID:Addgene_34695 Copy
Species: Homo sapiens
Genetic Insert: PRMT7
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX(SN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15044439
Proper citation: RRID:Addgene_34694 Copy
Species: Rattus norvegicus
Genetic Insert: PRMT1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8647869
Proper citation: RRID:Addgene_34699 Copy
Species: Homo sapiens
Genetic Insert: PRMT7
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX(SN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15044439
Proper citation: RRID:Addgene_34693 Copy
Species: Homo sapiens
Genetic Insert: TNKS2-M1054V
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11802774
Proper citation: RRID:Addgene_34692 Copy
Species: Homo sapiens
Genetic Insert: Sorting nexin 9
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17988218
Proper citation: RRID:Addgene_34690 Copy
Genetic Insert: FRB-LplA(W37I)
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22098454
Proper citation: RRID:Addgene_34840 Copy
Species: Rattus norvegicus
Genetic Insert: dynamin 2ba
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_34685 Copy
Species: Rattus norvegicus
Genetic Insert: dynamin 2ba
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_34684 Copy
Species: Homo sapiens
Genetic Insert: dynamin 1ba
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_34683 Copy
Species: Homo sapiens
Genetic Insert: dynamin 1
Vector Backbone Description: Vector Backbone:pUHD10-3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7559787
Proper citation: RRID:Addgene_34688 Copy
Species: Mus musculus
Genetic Insert: FKBP-LAP1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pcDNA4; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22098454
Proper citation: RRID:Addgene_34841 Copy
Species: Homo sapiens
Genetic Insert: dynamin 1ba
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_34682 Copy
Species: Homo sapiens
Genetic Insert: dynamin 1ba
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15004222
Proper citation: RRID:Addgene_34681 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:Derived from pBluescript II SK+; Vector Types:Hydra Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_34796 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:Derived from pBluescript II SK+; Vector Types:Hydra Expression; Bacterial Resistance:Ampicillin
Comments: https://www.ncbi.nlm.nih.gov/nuccore/158426709
Proper citation: RRID:Addgene_34794 Copy
Species: Mus musculus
Genetic Insert: Hoxd10
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:in situ hybridization probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16495162
Comments: This probe contains amplified sequence from the first exon of Hoxd10.
Proper citation: RRID:Addgene_34712 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript; Vector Types:Bacterial Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12399591
Comments: Use HT115 bacteria for experiments--Grow O/N on LB supplemented with carbenicillin (100ug/ml) & tetracyclin (12.5ug/ml) and pick single colony for liquid culture.
Proper citation: RRID:Addgene_34833 Copy
Species: Homo sapiens
Genetic Insert: PTD012
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_34797 Copy
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