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Species: Homo sapiens
Genetic Insert: PARP14
Vector Backbone Description: Backbone Marker:Opher Gileadi (Addgene plasmid # 26103); Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Use BL21(DE3)-R3-pRARE2 for expression. 5O2D: https://www.thesgc.org/structures/5O2D.
Proper citation: RRID:Addgene_110254 Copy
Species: Homo sapiens
Genetic Insert: IQSEC2
Vector Backbone Description: Vector Backbone:pNIC28-10His; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Use BL21 (DE3) Star for expression. PDB structure, 6FAE, is a complex of (ARF1A-c001 & IQSEC2A-c003).
Proper citation: RRID:Addgene_110259 Copy
Species: Homo sapiens
Genetic Insert: RG9MTD1
Vector Backbone Description: Backbone Marker:Opher Gileadi (Addgene plasmid # 26105); Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Use BL21(DE3)-R3-pRARE2 for expression. 5NFJ: https://www.thesgc.org/structures/5NFJ.
Proper citation: RRID:Addgene_110248 Copy
Species: Homo sapiens
Genetic Insert: FOXN1
Vector Backbone Description: Backbone Marker:Opher Gileadi (Addgene plasmid # 26103); Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Use BL21(DE3)-R3-pRARE2 for expression. 5OCN: https://www.thesgc.org/structures/5OCN.
Proper citation: RRID:Addgene_110249 Copy
Species: Homo sapiens
Genetic Insert: Yes
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110497 Copy
Species: Homo sapiens
Genetic Insert: PKCZ
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110512 Copy
Species: Homo sapiens
Genetic Insert: PLEKHS1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110510 Copy
Species: Homo sapiens
Genetic Insert: Rab13
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22674975
Proper citation: RRID:Addgene_110500 Copy
Species: Homo sapiens
Genetic Insert: CCDC32
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110505 Copy
Species: Homo sapiens
Genetic Insert: Rab5C-S35N
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22674975
Proper citation: RRID:Addgene_110504 Copy
Species: Homo sapiens
Genetic Insert: PLEKHS1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110509 Copy
Species: Homo sapiens
Genetic Insert: IQCF2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_110507 Copy
Species: Homo sapiens
Genetic Insert: NR4A1 homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR Blunt TOPO; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Cloning source: Human Sperm. Use with plasmid pNR4A1.1.0-gDNA.
Proper citation: RRID:Addgene_112295 Copy
Species: Homo sapiens
Genetic Insert: THRB homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Vector Backbone:pMB1 ori; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Cloning source: hg19 reference. Use with plasmid pTHRB.1.0-gDNA.
Proper citation: RRID:Addgene_112333 Copy
Species: Homo sapiens
Genetic Insert: ZNF408 homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Vector Backbone:pMB1 ori; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Cloning source: hg19 reference. Use with plasmid pZNF408.2.0-gDNA.
Proper citation: RRID:Addgene_112323 Copy
Species: Homo sapiens
Genetic Insert: ATF1 homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR Blunt TOPO; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Cloning source: Random Male Blood. Use with plasmid pATF1.1.0-gDNA.
Proper citation: RRID:Addgene_112328 Copy
Species: Homo sapiens
Genetic Insert: TEAD1 homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Vector Backbone:pMB1 ori; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Cloning source: hg19 reference. Use with plasmid pTEAD1.1.0-gDNA.
Proper citation: RRID:Addgene_112329 Copy
Species: Homo sapiens
Genetic Insert: ZNF398 homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Vector Backbone:pMB1 ori; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Cloning source: hg19 reference. Use with plasmid pZNF398.2.0-gDNA.
Proper citation: RRID:Addgene_112319 Copy
Species: Lactococcus lactis
Genetic Insert: HrtR
Vector Backbone Description: Backbone Size:2216; Vector Backbone:PZE2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29798884
Comments: Please note that Addgene NGS results identified an IS4-like element (ISVsa5 family) immediately downstream of ChuA. The depositing lab indicates that this is not expected to affect plasmid function.
Proper citation: RRID:Addgene_112532 Copy
Species: Lactococcus lactis
Genetic Insert: HrtR
Vector Backbone Description: Backbone Size:2216; Vector Backbone:PZE2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29798884
Comments: Blood sensor validated in murine and swine models.
The A17P mutation in ChuA is not expected to affect plasmid function.
Proper citation: RRID:Addgene_112533 Copy
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