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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pJT1
 
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RRID:Addgene_98299 PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B Saccharomyces cerevisiae Ampicillin PMID:27939849 Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence. Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:22:38 0
pPMVAd3
 
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RRID:Addgene_98298 PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A Saccharomyces cerevisiae Ampicillin PMID:27939849 Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence. Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:22:37 0
pPMVAd36
 
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RRID:Addgene_98302 PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1 Saccharomyces cerevisiae Ampicillin PMID:28239415 Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence. Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:22:38 0
pJT10R
 
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RRID:Addgene_98307 TRPL3AcNES1>TRPL41B Saccharomyces cerevisiae Ampicillin PMID:29471044 Please set 1/1/2018 as the initial day available for public access. Yeast promoters and terminators were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genom Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin ERG20 N127W 2026-08-15 01:22:37 0
pIMVAd40R
 
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RRID:Addgene_98309 ERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1 Saccharomyces cerevisiae Ampicillin PMID:29471044 Please set 1/1/2018 as the initial day available for public access. Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic seq Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:22:37 0
pZE21/UBP1/ClpS_V65I
 
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RRID:Addgene_98567 Truncated ubiquitin cleavase, codon optimized Saccharomyces cerevisiae Kanamycin PMID:29301968 Addgene NGS results found H571Y in the UBP1 translation compared to NP_010161.1. The depositing lab confirms that this mutation should not affect protein function Backbone Marker:Expressys; Backbone Size:2200; Vector Backbone:pZE21; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Removed the first 98 amino acids that encode transmembrane domains to improve yield as was done in literature (Wojtowicz et al, Microb Cell Fact, 2005, DOI: 10.1186/1475-2859-4-17) 2026-08-15 01:22:40 0
pZE21/UBP1/ClpS
 
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RRID:Addgene_98566 Truncated ubiquitin cleavase, codon optimized Saccharomyces cerevisiae Kanamycin PMID:29301968 Addgene NGS results found that the second tet operator in the tet promoter is missing; however the depositing laboratory confirmed that the plasmid functions normally Backbone Marker:Expressys; Backbone Size:2200; Vector Backbone:pZE21; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Removed the first 98 amino acids that encode transmembrane domains to improve yield as was done in literature (Wojtowicz et al, Microb Cell Fact, 2005, DOI: 10.1186/1475-2859-4-17) 2026-08-15 01:22:39 0
pRS313 - prp8 V1870N
 
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RRID:Addgene_90976 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin V1870N 2026-08-15 01:22:13 0
pRS313 - prp8 N1869D
 
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RRID:Addgene_90975 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin N1869D 2026-08-15 01:22:13 0
pRS313 - prp8 I1875T
 
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RRID:Addgene_90977 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin I1875T 2026-08-15 01:22:13 0
pRS313 - prp8 K1864E
 
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RRID:Addgene_90974 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin K1864E 2026-08-15 01:22:13 0
pRS313 - prp8 T1872E
 
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RRID:Addgene_90970 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin T1872E 2026-08-15 01:22:13 0
pRS313 - prp8 A1871E
 
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RRID:Addgene_90969 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin A1871E 2026-08-15 01:22:13 0
pRS313 - prp8 T1865K
 
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RRID:Addgene_90968 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin T1865K 2026-08-15 01:22:13 0
pRS313 - prp8 V1862D
 
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RRID:Addgene_90967 PRP8 Saccharomyces cerevisiae Ampicillin PMID:28416677 Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin V1862D 2026-08-15 01:22:13 0
pWS173
 
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RRID:Addgene_90960 Cas9 Saccharomyces cerevisiae Kanamycin For more information see https://benchling.com/pub/ellis-crispr-tools Backbone Size:4163; Vector Backbone:2 micron; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:22:13 0
pWS176
 
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RRID:Addgene_90963 Cas9 Saccharomyces cerevisiae Kanamycin For more information see https://benchling.com/pub/ellis-crispr-tools Backbone Size:3728; Vector Backbone:2 micron; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:22:13 0
CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)
 
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RRID:Addgene_91820 RPO21 Saccharomyces cerevisiae Ampicillin PMID:28826505 Backbone Marker:ATCC; Backbone Size:7771; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 5xGly linker, PreScission Protease site (LEVLFQGP), and 10xHis tag inserted between residues P1455 and E1456; changed lysine 1487 to asparagine and aspartate 1497 to lysine 2026-08-15 01:22:24 0
CPH3495 (pET151_Spt6 1247-1451 K1435A)
 
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RRID:Addgene_91817 SPT6 Saccharomyces cerevisiae Ampicillin PMID:28826505 Backbone Marker:Thermo Fisher Scientific; Backbone Size:5766; Vector Backbone:pET151/D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin changed lysine 1435 to alanine; deleted residues 1-1246 2026-08-15 01:22:24 0
CPH1141 (pET151_Spt6 1247-1451)
 
Resource Report
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RRID:Addgene_91815 SPT6 Saccharomyces cerevisiae Ampicillin PMID:28826505 Backbone Marker:Thermo Fisher Scientific; Backbone Size:5766; Vector Backbone:pET151/D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin deleted residues 1-1246 2026-08-15 01:22:24 0

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