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Species: Synthetic
Genetic Insert: F3-flanked spectinomycin resistance cassette
Vector Backbone Description: Backbone Marker:KEIO collection material; Backbone Size:2000; Vector Backbone:pKD13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38488830
Comments: Please visit https://doi.org/10.1101/2024.01.17.576040 for bioRxiv preprint.
Proper citation: RRID:Addgene_127551 Copy
Species: Synthetic
Genetic Insert: F5-flanked tetracycline resistance cassette
Vector Backbone Description: Backbone Marker:KEIO collection material; Backbone Size:2000; Vector Backbone:pKD13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_127552 Copy
Species: Synthetic
Genetic Insert: PtetO7.1_Flagtag_8Glycine linker
Vector Backbone Description: Backbone Size:2894; Vector Backbone:pFA6; Vector Types:Synthetic Biology, PCR based tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34342575
Comments: For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
Proper citation: RRID:Addgene_127581 Copy
Species: Synthetic
Genetic Insert: PtetO7.1_Flagtag_8Glycine linker
Vector Backbone Description: Backbone Size:3350; Vector Backbone:pFA6; Vector Types:Synthetic Biology, PCR based tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34342575
Comments: For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
Proper citation: RRID:Addgene_127580 Copy
Species: Synthetic
Genetic Insert: PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
Vector Backbone Description: Backbone Size:3748; Vector Backbone:pRG203MX; Vector Types:Yeast Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34342575
Comments: For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
Proper citation: RRID:Addgene_127577 Copy
Species: Synthetic
Genetic Insert: PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
Vector Backbone Description: Backbone Size:5108; Vector Backbone:pRG206; Vector Types:Yeast Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34342575
Comments: For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
Proper citation: RRID:Addgene_127575 Copy
Species: Synthetic
Genetic Insert: 5HT2A_NB2
Vector Backbone Description: Vector Backbone:pCDNA3.1 hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31280962
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_127690 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-R72K
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127683 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y66F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127681 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y63F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127680 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y98F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127686 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y76F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127684 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-P399A
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127689 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127679 Copy
Species: Synthetic
Genetic Insert: dCas9-CIBN-2A-Puro
Vector Backbone Description: Backbone Marker:Cremins lab; Backbone Size:3276; Vector Backbone:Derived from pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235883
Proper citation: RRID:Addgene_127664 Copy
Species: Synthetic
Genetic Insert: CRY2-HA-2A-mCherry and gRNAs 115 and 117
Vector Backbone Description: Backbone Marker:Cremins lab; Backbone Size:4437; Vector Backbone:Derived from pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235883
Proper citation: RRID:Addgene_127669 Copy
Species: Synthetic
Genetic Insert: CRY2-HA-2A-mCherry
Vector Backbone Description: Backbone Marker:Cremins lab; Backbone Size:3247; Vector Backbone:Derived from pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235883
Proper citation: RRID:Addgene_127667 Copy
Species: Synthetic
Genetic Insert: gRNAs 129, 135, 115, 117
Vector Backbone Description: Backbone Marker:Cremins lab; Backbone Size:3308; Vector Backbone:Derived from pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235883
Proper citation: RRID:Addgene_127666 Copy
Species: Synthetic
Genetic Insert: GFP1-10
Vector Backbone Description: Backbone Size:5582; Vector Backbone:Pmyo-3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30952669
Proper citation: RRID:Addgene_127706 Copy
Species: Synthetic
Genetic Insert: GFP1-10
Vector Backbone Description: Backbone Size:3972; Vector Backbone:Pttr-39; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30952669
Proper citation: RRID:Addgene_127704 Copy
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