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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pYC46 Resource Report Resource Website |
RRID:Addgene_63904 | Ampicillin | PMID:25986172 | Backbone Size:5915; Vector Backbone:pYC44; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:06 | 0 | ||||
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pYC50 Resource Report Resource Website |
RRID:Addgene_63906 | Ampicillin | PMID:25986172 | Backbone Size:5915; Vector Backbone:pYC44; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | ||||
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pST50Trc4-CBPHISNDHFR Resource Report Resource Website |
RRID:Addgene_63999 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pST50Trc4-CBPNDHFR Resource Report Resource Website |
RRID:Addgene_63995 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pST50Trc4-FLAGNDHFR Resource Report Resource Website |
RRID:Addgene_63997 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:08 | 0 | |||
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pcDNA3-EngPDm Resource Report Resource Website |
RRID:Addgene_64040 | Pax8 paired domain | Mus musculus | Ampicillin | PMID:17182173 | There is a BamHI site in between the Eng and PDm coding sequences. Drosophila Engrailed (Eng)contains H22P and L158P compared to closest reference sequence (AAA65478.1). Depositor states that these mutation should not affect function. Mouse Pax8 contains a P2A mutation. Depositor states that this mutation should not affect plasmid function. | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | paired domain, aa 1-147, with aa 30 to 32 changed to Ala (creating a NotI restriction site) | 2026-08-15 01:18:08 | 0 |
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pST50Trc4-STRDHFR Resource Report Resource Website |
RRID:Addgene_63991 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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PX458_CEBPB_2 Resource Report Resource Website |
RRID:Addgene_64047 | gRNA | Ampicillin | PMID:26355004 | Confirmed by the Mendenhall and Myers labs to Flag epitope tag the endogenous human CEBPB gene when pooled with plasmids PX548_CEBPB_1 and pFETCH_CEPBP and transfected into human cells. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details. | Backbone Size:9289; Vector Backbone:px548; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:08 | 0 | ||
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pST50Trc4-FLAGDHFR Resource Report Resource Website |
RRID:Addgene_63992 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pLenti-myc-GLUT4-mCherry Resource Report Resource Website 10+ mentions |
RRID:Addgene_64049 | GLUT4 | Mus musculus | Ampicillin | PMID:25575350 | The c-Myc sequence was inserted into the region encoding the 1st exofacial loop of mouse GLUT4 in the background of pmCherry-N1. The fragment of Myc-GLUT4-mCherry was then sub-cloned into pLenti-hiko vector via NheI + HpaI digestion. Discrepancies between the Addgene QC sequence and the full sequence are not of functional concern. Please cite the following article: Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015). | Backbone Size:9124; Vector Backbone:pLenti-hiko; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:08 | 12 | |
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pST50Trc4-HISNDHFR Resource Report Resource Website |
RRID:Addgene_63994 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pLenti-Lifeact-tdTomato Resource Report Resource Website 1+ mentions |
RRID:Addgene_64048 | Lifeact | Saccharomyces cerevisiae | Ampicillin | PMID:25575350 | The Lifeact sequence was initially reported in Riedl, J. et al. Lifeact: a versatile marker to visualize F-actin. Nat Methods 5, 605–607 (2008). Please cite the following article as follows: Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015). | Backbone Size:9124; Vector Backbone:pLenti-hiko; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:08 | 6 | |
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pCfB2793 Resource Report Resource Website |
RRID:Addgene_64043 | GFP | Other | Ampicillin | PMID:26934490 | Please see previous FEMS Yeast Research paper for more details: http://femsyr.oxfordjournals.org/content/14/2/238 Feature list: T CYC1 Terminator 6..195 T K.l. URA3 Terminator 303..425 Degradation tag 426..476 K. lactis URA3 CDS without STOP codon 477..1277 K. lactis URA3 promoter 1278..1776 Consensus Ty2 3 1966..2152 pUC ori 2939..3582 Consensus Ty2 5 4987..5215 T ADH1 Terminator 5242..5436 pTEF1 Promoter 5455..5874 GFP CDS 5884..6600 | Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:08 | 0 | |
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pST50Trc4-CBPDHFR Resource Report Resource Website |
RRID:Addgene_63990 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pLenti-FLAG-Akt2-CA Resource Report Resource Website |
RRID:Addgene_64050 | AKT2 | Homo sapiens | Ampicillin | PMID:25575350 | The MYR sequence was inserted in frame at EcoRI/NheI site into N terminal of pLenti-FLAG-hAkt2 Please cite the following article: Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015). | Backbone Size:9124; Vector Backbone:pLenti-hiko; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:08 | 0 | |
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EcM2.1.dtolC Resource Report Resource Website 1+ mentions |
RRID:Addgene_64053 | see comments | Ampicillin | PMID:24452804 | Escherichia coli MG1655 mutS_fs dnaG_Q576A exoX- xonA- xseA- 1255700::tolQRA Δ(ybhB-bioAB)::[λcI857 N(cro-ea59)::tetR-bla] tolC- | Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:08 | 1 | ||
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pFETCh_RAD21 Resource Report Resource Website |
RRID:Addgene_64056 | RAD21 Homology Arms | Homo sapiens | Kanamycin | PMID:26355004 | This plasmid has been used along with PX548_RAD21_1 to add a FLAG tag to human RAD21 in HepG2 and MCF7 cells by the Mendenhall and Myers labs at HudsonAlpha Institute for Biotechnology/UAH. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details. | Backbone Size:5771; Vector Backbone:pFETCH_Donor; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:18:08 | 0 | |
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L304-EGFP-Tubulin-WT Resource Report Resource Website 10+ mentions |
RRID:Addgene_64060 | tubulin | Ampicillin | PMID:23126280 | Addgene sequencing results identified a duplication of the EGFP-Tubulin insert. It is not known how this impacts plasmid function. | Vector Backbone:L304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:08 | 14 | ||
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L309-myc-seipin-CT Resource Report Resource Website |
RRID:Addgene_64063 | bscl2 | Mus musculus | Ampicillin | PMID:23458123 | Vector Backbone:L309; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | Contains Arg326 to Ser443 | 2026-08-15 01:18:08 | 0 | |
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L309-myc-seipin-ΔCT Resource Report Resource Website |
RRID:Addgene_64062 | bscl2 | Mus musculus | Ampicillin | PMID:23458123 | Vector Backbone:L309; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | Deletion of C-terminal Asp338–Ser443 | 2026-08-15 01:18:08 | 0 |
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