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Species: Homo sapiens
Genetic Insert: VPS29
Vector Backbone Description: Backbone Size:4700; Vector Backbone:YFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18981234
Proper citation: RRID:Addgene_174520 Copy
Species: Butyrivibrio fibrisolvens
Genetic Insert: huBfCas12a
Vector Backbone Description: Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: The vector is obtained from Scott Gradia Lab (Addgene #29656) while the gene is obtained from Feng Zhang Lab (Addgene #92301) . Read the preprint on medRxiv here: https://www.medrxiv.org/content/10.1101/2021.07.21.21260653v1
Proper citation: RRID:Addgene_174672 Copy
Species: Moraxella bovoculi AAX08_00205
Genetic Insert: huMb2Cas12a
Vector Backbone Description: Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: The vector is obtained from Scott Gradia Lab (Addgene #29656) while the gene is obtained from Feng Zhang Lab (Addgene #92292) . Read the preprint on medRxiv here: https://www.medrxiv.org/content/10.1101/2021.07.21.21260653v1
Proper citation: RRID:Addgene_174676 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3730; Vector Backbone:pGL4.10; Vector Types:Enhancer reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34874265
Proper citation: RRID:Addgene_174669 Copy
Genetic Insert: Enhanced green fluorescent protein shRNA #1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pENTR1A; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Comments: shRNA oligo sequence 5'-CGAAGCAGCACGACTTCTTCTTCAAGAGAGAAGAAGTCGTGCTGCTTCTTTTT
Proper citation: RRID:Addgene_17470 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3023; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression, Plant Expression, modified Gateway Entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18211686
Comments: Part of a modified Gateway Entry vector system for generating epitope tagged constructs. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a C-terminal ECFP tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.
The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).
GenBank accession number: EU344826
Proper citation: RRID:Addgene_17465 Copy
Species: Synthetic
Genetic Insert: LUC2
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_174665 Copy
Species: Homo sapiens
Genetic Insert: ubiquitination sgRNA
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52961); Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31692446
Proper citation: RRID:Addgene_174592 Copy
Genetic Insert: GM-CSF
Vector Backbone Description: Vector Backbone:mp71; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34396986
Proper citation: RRID:Addgene_174596 Copy
Species: Mus musculus
Genetic Insert: KIF1A(1-365)-GFP-SSPB(micro)
Vector Backbone Description: Vector Backbone:B-actin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32328628
Comments: Internal plasmid ID: WN233
Proper citation: RRID:Addgene_174629 Copy
Species: Homo sapiens
Genetic Insert: Rec8
Vector Backbone Description: Vector Backbone:pBABE-Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34331869
Comments: pNM937 . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
Proper citation: RRID:Addgene_174717 Copy
Species: Homo sapiens
Genetic Insert: P100
Vector Backbone Description: Vector Backbone:pCLXSN(GFP); Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_174734 Copy
Vector Backbone Description: Backbone Size:4506; Vector Backbone:pKO403; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35023783
Proper citation: RRID:Addgene_174726 Copy
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2240; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17473 Copy
Species: Homo sapiens
Genetic Insert: PARP1deltaART
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-784 (V762A)
Proper citation: RRID:Addgene_174804 Copy
Vector Backbone Description: Backbone Size:8007; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17482 Copy
Species: Homo sapiens
Genetic Insert: PE2-P2A-hMLH1dn
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34653350
Proper citation: RRID:Addgene_174827 Copy
Species: Mus musculus
Genetic Insert: Cre flanked by MmPeg10 UTRs
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34413232
Proper citation: RRID:Addgene_174862 Copy
Species: Methanosarcina mazei
Genetic Insert: Mma PylRS
Vector Backbone Description: Backbone Marker:Addgene #140008 based on PB510B-1 (System Bioscience); Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34431592
Proper citation: RRID:Addgene_174890 Copy
Genetic Insert: Tetracycline repressor
Vector Backbone Description: Backbone Size:8477; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17492 Copy
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