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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pcDNA3.1 AKT3 HA (CT-gfp) Resource Report Resource Website 1+ mentions |
RRID:Addgene_27293 | protein kinase B gamma | Mus musculus | Ampicillin | The GFP is not expressed as there is a stop codon after the HA tag. mTORC2 reportedly can phosphorylate FSY and FSF sites, so the Y473F mutation should not affect expression or function. | Backbone Marker:Invitrogen; Backbone Size:6157; Vector Backbone:pcDNA3.1/CT-GFP-Topo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Y473F | Addgene | 2026-10-31 02:08:20 | 2 | |
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pEF1-alpha-V Resource Report Resource Website 1+ mentions |
RRID:Addgene_27290 | integrin alpha V | Homo sapiens | Ampicillin | PMID:12230977 | Backbone Marker:Invitrogen; Backbone Size:6200; Vector Backbone:pEF1/V5-HisA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Isoform 2 | Addgene | 2026-10-31 02:08:20 | 6 | |
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dpf2_R (OZ532) Resource Report Resource Website |
RRID:Addgene_27209 | Zinc finger array targeting dpf2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dpf2 (see below). Please note that this plasmid does NOT contain the dpf2 sequence. Users must order the complementary plasmid dpf2_L (OZ531) [Addgene plasmid 27208] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGAGAAACG-3' in a bacterial two hybrid assay, and resulted in 5.71 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:20 | 0 | |
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dpf2_L (OZ531) Resource Report Resource Website |
RRID:Addgene_27208 | Zinc finger array targeting dpf2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dpf2 (see below). Please note that this plasmid does NOT contain the dpf2 sequence. Users must order the complementary plasmid dpf2_R (OZ532) [Addgene plasmid 27209] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAAGTAGCA-3' in a bacterial two hybrid assay, and resulted in 8.58 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:20 | 0 | |
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mdka (midkine-related growth factor)_R (OZ528) Resource Report Resource Website |
RRID:Addgene_27205 | Zinc finger array targeting mdka | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene mdka (see below). Please note that this plasmid does NOT contain the mdka sequence. Users must order the complementary plasmid mdka (midkine-related growth factor)_L (OZ527) [Addgene plasmid 27204] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGGTGGC-3' in a bacterial two hybrid assay, and resulted in 3.75 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:20 | 0 | |
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pMLM802 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27203 | FokI endonuclease | Ampicillin | PMID:17603475 | For expression of a "-" heterodimeric FokI domain. (Miller et al., Nat Biotech 2007) in mammalian cells or zebrafish for ZFN targets with a 7bp spacer (Handel et al 2008, Mol Ther). To clone a zinc finger array into this plasmid, perform the following steps: (1) amplify the zinc finger array coding sequence from a B2H expression vector by PCR using primers OMM429 (5’-GATGACAAATCTAGACCCGGGGAGCG-3’) and OMM430 (5’-CTGCGGCCGCACCGGGTCCTGTGTGGGTTTTTAGGTG-3’), (2) digest the resulting PCR fragment with XbaI and NotI, and (3) clone the digested fragment into MLM802 vector backbone that has been digested with XbaI and NotI. The resulting plasmid will encode a ZFN that can be paired with a ZFN cloned into expression vector MLM800 to target a ZFN target site with a 7 bp spacer (Handel et al. 2008, Mol Ther.). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. | Backbone Size:5727; Vector Backbone:pMLM802; Vector Types:Mammalian Expression, zebrafish expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:20 | 1 | ||
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mdka (midkine-related growth factor)_L (OZ527) Resource Report Resource Website |
RRID:Addgene_27204 | Zinc finger array targeting mdka | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene mdka (see below). Please note that this plasmid does NOT contain the mdka sequence. Users must order the complementary plasmid mdka (midkine-related growth factor)_R (OZ528) [Addgene plasmid 27205] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGAGGGT-3' in a bacterial two hybrid assay, and resulted in 7.20 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:20 | 0 | |
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pDestTol2pA2_ubi:EGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_27323 | ubiquitin:EGFP reporter | Danio rerio | Ampicillin | PMID:21138979 | There are a few nucleotide discrepancies between author sequence and Addgene quality control sequence. According to the depositing lab, these do not affect plasmid function. | Backbone Size:4179; Vector Backbone:pDestTol2A2; Vector Types:Tol2 transposon; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:21 | 5 | |
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pENTR5'_ubi Resource Report Resource Website 10+ mentions |
RRID:Addgene_27320 | zebrafish ubiquitin promoter | Danio rerio | Kanamycin | PMID:21138979 | Backbone Marker:Invitrogen; Backbone Size:2692; Vector Backbone:pENTR5' TOPO; Vector Types:Multisite Gateway 5' entry vector; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:21 | 25 | ||
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pEF1-alpha-IIb Resource Report Resource Website 1+ mentions |
RRID:Addgene_27288 | integrin alpha IIb | Homo sapiens | Ampicillin | PMID:12230977 | Backbone Marker:Invitrogen; Backbone Size:6200; Vector Backbone:pEF1/V5-HisA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | n/a | Addgene | 2026-10-31 02:08:20 | 1 | |
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LRP6-m5-pCS2-VSVG Resource Report Resource Website 1+ mentions |
RRID:Addgene_27283 | low density lipoprotein receptor-related protein 6 | Homo sapiens | Ampicillin | PMID:14731402 | Dominant inhibitor of Wnt function, similar to LRPT-dC | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:xenopus injection; Bacterial Resistance:Ampicillin | Changed S/T of all five PPP(S/T)P motifs to Alanine. 2 of of the 5 sites deviate slightly from the consensus sequence (there is one PPAAP and one CPPAP) | Addgene | 2026-10-31 02:08:20 | 2 |
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p2Flag CMV2-ZO-2-delta-N Resource Report Resource Website |
RRID:Addgene_27419 | Zonula Occludens 2 | Homo sapiens | Ampicillin | PMID:20868367 | Backbone Size:4700; Vector Backbone:p2xFlag CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Deletion of coding nucleotides from 1-351. This results in N-terminal deletion of 117 amino acids. See Figure 3 in the paper for details. | Addgene | 2026-10-31 02:08:22 | 0 | |
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pMRS156.2 (miR156 sensor) Resource Report Resource Website |
RRID:Addgene_27412 | miR156 target site | Kanamycin | PMID:21123653 | The miR156 sensor construct was created by cloning miR156 target sites into the control sensor plasmid (Addgene Plasmid # 27405). miR156 target site in the 5' untranslated region (UTR) of a ubiquitously expressed nuclear-localized GFP. | Backbone Size:0; Vector Backbone:pCGTAG; Vector Types:; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:22 | 0 | ||
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pCMV-Flag YAP127/128/131/381A Resource Report Resource Website 1+ mentions |
RRID:Addgene_27378 | YAP | Homo sapiens | Ampicillin | PMID:20048001 | Backbone Size:5000; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S127/128/131/381A | Addgene | 2026-10-31 02:08:21 | 2 | |
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SPL11p::pCGTAG (SPL11p::NLS-GFP) Resource Report Resource Website |
RRID:Addgene_27411 | SPL11 promoter | Arabidopsis thaliana | Kanamycin | PMID:21123653 | SPL11p::NLS-GFP was generated by PCR amplification of 2.2-kb genomic DNA. The resulting amplicon was then cloned into pCR8/GW-TOPO (Invitrogen) and recombined with pCGTAG. The primers used to create this construct were 5'-AAATCTCGTCCAACT-3' and 5'-TCACGATATGGGTTG-3'. | Backbone Size:0; Vector Backbone:pCGTAG; Vector Types:; Bacterial Resistance:Kanamycin | SPL11 promoter transcriptionally fused to a nuclear-localized GFP | Addgene | 2026-10-31 02:08:22 | 0 |
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pGMCtN-P38-sspBmyc Resource Report Resource Website |
RRID:Addgene_27375 | sspBmyc | Nourseothricin (clonNat) | PMID:19174563 | Backbone Marker:N/A; Backbone Size:3725; Vector Backbone:pDE43-MCtNq1; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Nourseothricin (clonNat) | N/A | Addgene | 2026-10-31 02:08:21 | 0 | ||
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pGMEH-10M1-clpX-K127R Resource Report Resource Website |
RRID:Addgene_27374 | tetR(sB), clpXtb-K127R | Hygromycin | PMID:19174563 | Backbone Marker:N/A; Backbone Size:6181; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Hygromycin | changed Lysine 127 to Arginine | Addgene | 2026-10-31 02:08:21 | 0 | ||
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pBB535 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27392 | dnaK, dnaJ | E. coli | Spectinomycin | PMID:17565681 | Please note that Addgene's sequencing results detect a single nucleotide insertion compared to depositor's sequence in the vector backbone. The plasmid expresses the expected functional protein. | Backbone Size:7072; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | Addgene | 2026-10-31 02:08:21 | 1 | |
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pBB528 Resource Report Resource Website |
RRID:Addgene_27390 | lacIq | E. coli | Chloramphenicol | PMID:17565681 | Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. | Backbone Size:4945; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | Addgene | 2026-10-31 02:08:21 | 0 | |
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pGEX-4T3-ZO-2-1st-PDZ-delta-NLS Resource Report Resource Website |
RRID:Addgene_27425 | Zonula Occludens 2 | Homo sapiens | Ampicillin | PMID:20868367 | Backbone Marker:Pharmacia; Backbone Size:4969; Vector Backbone:pGEX-4T3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Deletion of NLS-Nuclear Localization Signal: Nucleotides 313-873, counting A in the ATG start codon as 1, are deleted. | Addgene | 2026-10-31 02:08:22 | 0 |
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