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Species: Saccharomyces cerevisiae
Genetic Insert: BUD32
Vector Backbone Description: Backbone Size:6021; Vector Backbone:pRS415; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10991 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hUD recombination system
Vector Backbone Description: Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: The repeats of HIS3 in this plasmid contain truncations of the HIS3 gene. The first repeat is a 3' end truncation that contains the promoter and the ORF from the ATG to the internal KpnI site (used for subcloning). The second repeat is a 5' truncation that contains from the internal NdeI site (used for subcloning) to the end of the gene. Recombination of the common DNA sequence between these repeats (approx. 430 nt internal to the HIS3 ORF) reconstitutes a functional gene. Both plasmids, containing the repeats in direct orientation (hUD) or inverted orientation (hUI), have been tested for recombination with positive result.
Proper citation: RRID:Addgene_11005 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3
Vector Backbone Description: Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11004 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hUI recombination system
Vector Backbone Description: Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: The repeats of HIS3 in this plasmid contain truncations of the HIS3 gene. The first repeat is a 3' end truncation that contains the promoter and the ORF from the ATG to the internal KpnI site (used for subcloning). The second repeat is a 5' truncation that contains from the internal NdeI site (used for subcloning) to the end of the gene. Recombination of the common DNA sequence between these repeats (approx. 430 nt internal to the HIS3 ORF) reconstitutes a functional gene. Both plasmids, containing the repeats in direct orientation (hUD) or inverted orientation (hUI), have been tested for recombination with positive result.
Proper citation: RRID:Addgene_11006 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSM1
Vector Backbone Description: Backbone Size:6021; Vector Backbone:pRS415; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11003 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSM1
Vector Backbone Description: Backbone Size:5741; Vector Backbone:YEplac181; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11002 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pgal1TRP1URA3
Vector Backbone Description: Backbone Size:3786; Vector Backbone:pYC6-CT/HIS3 hybrid; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Please note that Addgene's sequencing results identified mutations A160S and K173R in the URA3 sequence. These mutations were not intentional and the depositing scientist states that the plasmid was tested for complementation of URA and TRP phenotypes and for production of a single transcript.
Proper citation: RRID:Addgene_11009 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1
Vector Backbone Description: Backbone Size:4967; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29122887
Comments: The 3788 bp insert covers entire PRT module.For cloning of gene of interest, just use Asc I/Bam HI sites.
Proper citation: RRID:Addgene_104342 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MDH2
Vector Backbone Description: Backbone Size:8017; Vector Backbone:pJO629; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28126757
Comments: Replace the Yap5 gene of pJO629 to MDH1 gene.
Proper citation: RRID:Addgene_104344 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-NLS-2xyeGFP
Vector Backbone Description: Vector Backbone:YcpLac111; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131164
Comments: Insert expressed under the control of the CYC1 promoter and terminator
Proper citation: RRID:Addgene_104394 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL13Ap
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26709839
Proper citation: RRID:Addgene_104474 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL13Ap
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26709839
Proper citation: RRID:Addgene_104473 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OM45p
Vector Backbone Description: Backbone Size:3545; Vector Backbone:YIplac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26709839
Proper citation: RRID:Addgene_104472 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScPGM2 promoter-yEGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29301066
Proper citation: RRID:Addgene_104502 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Tlg1
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Comments: Addgene's quality control identified G264D in URA3. The depositing lab states that this mutation should not affect selection.
Proper citation: RRID:Addgene_105261 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Arf1
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105255 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Chc1 portion
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105256 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Apl5
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105253 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Apl6 portion
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105254 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: APL2 portion
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105251 Copy
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