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Species: Synthetic
Genetic Insert: GFP1-10
Vector Backbone Description: Backbone Size:6564; Vector Backbone:Pacr-2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30952669
Proper citation: RRID:Addgene_127703 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Backbone Size:2200; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29564885
Comments: The insert mTq2-Tat was designed by G.Grossi and M. Schwarz-Schilling and cloned by A. Dupin and M. Schwarz-Schilling. mTq2 sequence is from Goedhart, J. et al. Nat. Commun. 2012, 3, 751. Tat sequence is from Bayer, T. S. et al. RNA 2005, 11, 1848−57. The peptide linker sequence between between YPet and PCP is TRGGGGS.
Proper citation: RRID:Addgene_127635 Copy
Species: Synthetic
Genetic Insert: RNA nanostrcutre
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29564885
Comments: The insert was designed by F. Chizzolini and M. Schwarz-Schilling and cloned by M.Schwarz-Schilling. The RNA sequence for the: 3WJ is from Shu, D. et al. Nucleic Acids Res. 2014, 42, e10; MG aptamer is from Babendure, J. R. et al. J. Am. Chem. Soc. 2003, 125, 14716−7; PP7 aptamer is from Chao, J. A. et al. Nat. Struct. Mol. Biol. 2008, 15, 103−5; TAR aptamer is from Bayer, T. S. et al. RNA 2005, 11, 1848−57; Streptavidin aptamer is from: Srisawat, C. and Engelke, D. R. RNA 2001, 7, 632−41.
Proper citation: RRID:Addgene_127634 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:1933; Vector Backbone:pMB1 zeocin; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_127638 Copy
Species: Synthetic
Genetic Insert: EGFP-NLS
Vector Backbone Description: Backbone Size:9004; Vector Backbone:pRG01; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30962622
Proper citation: RRID:Addgene_123363 Copy
Species: Synthetic
Genetic Insert: Venus-Venus-FLARE-Cameleon
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29968564
Proper citation: RRID:Addgene_123351 Copy
Species: Synthetic
Genetic Insert: Venus-cpVenus-FLARE-Cameleon
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29968564
Proper citation: RRID:Addgene_123350 Copy
Species: Synthetic
Genetic Insert: mCherry-mCherry-FLARE-Cameleon
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29968564
Proper citation: RRID:Addgene_123352 Copy
Species: Synthetic
Genetic Insert: cp-moxCerulean3-moxCerulean3-FLARE-D1ER
Vector Backbone Description: Backbone Size:3933; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29968564
Proper citation: RRID:Addgene_123354 Copy
Species: Synthetic
Genetic Insert: human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium,
Vector Backbone Description: Backbone Size:10130; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30962622
Proper citation: RRID:Addgene_123359 Copy
Species: Synthetic
Genetic Insert: Venus-cpVenus-FLARE-EKAR-EV(T/A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29968564
Proper citation: RRID:Addgene_123340 Copy
Species: Synthetic
Genetic Insert: NLS-nCas9-NLS-P2A-EGFP
Vector Backbone Description: Backbone Marker:pCMV-BE1 (Addgene #73019); Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30995674
Proper citation: RRID:Addgene_123616 Copy
Species: Synthetic
Genetic Insert: nCas9-UGI-NLS-P2A-EGFP
Vector Backbone Description: Backbone Marker:pSQT817 (Addgene #53373); Backbone Size:4843; Vector Backbone:CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30995674
Proper citation: RRID:Addgene_123611 Copy
Species: Synthetic
Genetic Insert: ArchT-tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4763; Vector Backbone:AAV-Syn-FLEX; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21811444
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_123607 Copy
Species: Synthetic
Genetic Insert: FLARE16-S aadA-GFP
Vector Backbone Description: Vector Backbone:pMK-T; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30679266
Comments: All inserts have been produced by gene synthesis and cloned in the indicated backbone vector. This insert is a coding sequence designed for Golden-Gate cloning. The Original Material was developed in the performance of research that was funded by Grant Nos. ARPA-E: DE-AR000660 & DARPA: D17AC00016.
Proper citation: RRID:Addgene_123562 Copy
Species: Synthetic
Genetic Insert: crRNA scaffold 4n96
Vector Backbone Description: Vector Backbone:pUC19, BsaI site mutated; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30717767
Proper citation: RRID:Addgene_123640 Copy
Species: Synthetic
Genetic Insert: AsCas12a
Vector Backbone Description: Vector Backbone:pCAG-2AeGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30717767
Proper citation: RRID:Addgene_123638 Copy
Species: Synthetic
Genetic Insert: BsCas12a
Vector Backbone Description: Vector Backbone:pCAG-2AeGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30717767
Proper citation: RRID:Addgene_123633 Copy
Species: Synthetic
Genetic Insert: LpCas12a
Vector Backbone Description: Vector Backbone:pCAG-2AeGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30717767
Proper citation: RRID:Addgene_123635 Copy
Species: Synthetic
Genetic Insert: AnnexinA5
Vector Backbone Description: Vector Backbone:pDEST-Tol2-pA2 a-cry-mCherry; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_123628 Copy
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