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  • Organism:mus musculus (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
mId1XGFP
 
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RRID:Addgene_20965 Inhibitor of DNA binding 1 Mus musculus Kanamycin PMID:19896442 Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:11:58 0
mId1-GFP
 
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1+ mentions
RRID:Addgene_20964 Inhibitor of DNA binding 1 Mus musculus Kanamycin PMID:19896442 Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:11:58 1
HOXA9-MSCV-full-GFP
 
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1+ mentions
RRID:Addgene_20977 HOXA9 Mus musculus Ampicillin PMID:18474618 Addgene has detected several mutations in the UTR region of this construct. Backbone Size:6500; Vector Backbone:MSCV-PGK-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin Full length cDNA with ~1.1kb 3'UTR with 2 putative polyA signals mutated to allow expression 2026-08-15 01:11:58 2
HOXA9-MSCV-CD24
 
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RRID:Addgene_20975 HOXA9 Mus musculus Ampicillin PMID:18474618 *Please note that the full sequence verified by Addgene differs from the original depositor's map and thus the numbers and nomenclature used by the depositor may differ from the Addgene map.* Depositor's Comments: We discovered that, although a 1,975-nt full-length Hoxa9 clone (numbered from the protein start site; I.M.A.G.E. clone BC055059) would produce protein when transiently expressed from the murine stem cell virus (MSCV) long terminal repeat, following retroviral transduction, the HOXA9 protein could not be detected. Since this cDNA contained two putative polyadenylation signals in the 3' UTR, we removed these sites to determine if they were interfering with retrovirally mediated protein expression. The site at nt 1798 was replaced using a primer containing a KpnI sequence in place of the AATAAA sequence together with a primer containing the BglII site at nt 749 to amplify a 1,052-nt BglII-KpnI fragment by PCR. A second primer containing the KpnI site in place of the polyadenylation signal at nt 1798 was used in conjunction with a primer containing an AAT-to-CGG change in the polyadenylation signal at nt 1975 and an adjacent XhoI site for cloning to generate a 150-nt KpnI-XhoI fragment. Standard methods were used to combine these fragments to create a 1,975-nt Hoxa9 cDNA encoding an N-terminal FLAG-tagged version of the mature protein but lacking the two putative polyadenylation signals. The resulting MSCV clone produced high levels of FLAG-HOXA9 protein and immortalized BM cells. Vector Backbone:MSCV (murine stem cell virus); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin Full length cDNA with ~1.1kb 3'UTR with 2 putative polyA signals mutated to allow expression in MSCV+CD24 selection epitope (FACS surface marker). No polyA signal. 2026-08-15 01:11:58 0
PB-TET-MKOS
 
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RRID:Addgene_20959 cMyc, KLF4, Oct4, Sox2 Mus musculus Ampicillin PMID:19252478 Additional reference, Kaji et al Nature. 2009 Mar 1 Backbone Size:9186; Vector Backbone:PB-TET; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin 2026-08-15 01:11:58 8
PB-TET-mcMyc
 
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1+ mentions
RRID:Addgene_20888 cMyc Mus musculus Ampicillin PMID:19252478 Backbone Size:9186; Vector Backbone:PB-TET; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin 2026-08-15 01:11:58 1
pGL-p21UTRm1&2
 
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RRID:Addgene_20880 p21 3'UTR site 1 and 2 mutation Mus musculus Ampicillin PMID:18978791 Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin Predicted miRNA binding site 1 (Position ~420) GCACTTT to GCAATGT Predicted miRNA binding site 2 (Position ~1100) GCACTTA to GCCCGTA 2026-08-15 01:11:58 0
pGL-RbUTR
 
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RRID:Addgene_20883 Rb 3'UTR Mus musculus Ampicillin PMID:18978791 One C->A mutation is present but does affect function of plasmid. Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:11:58 0
HOXB9-pBLSK
 
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RRID:Addgene_20997 HOXB9 Mus musculus Ampicillin PMID:1681962 Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:59 0
PRRX2-pET
 
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RRID:Addgene_21011 PRRX2 Mus musculus Kanamycin PMID:9665387 Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Missing first 3 N-terminal amino acids 2026-08-15 01:11:59 0
PRRX2-MSCV
 
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1+ mentions
RRID:Addgene_21010 PRRX2 Mus musculus Ampicillin PMID:9665387 Backbone Marker:Clontech; Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:11:59 2
CBP-HAT-GST
 
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1+ mentions
RRID:Addgene_21093 CBP Mus musculus Ampicillin PMID:11585930 The protein product of this clone exhibits good HAT activity on histone substrates. Backbone Marker:Amersham; Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin HAT domain: aa 1196-1718 2026-08-15 01:12:00 1
CBP-CH3-SP65
 
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RRID:Addgene_21092 CBP Mus musculus Ampicillin PMID:11585930 Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP65; Vector Types:in vitro TNT; Bacterial Resistance:Ampicillin CH3 domain aa 1625-1877 2026-08-15 01:11:59 0
CBP-HAT-pET
 
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RRID:Addgene_21091 CBP Mus musculus Kanamycin PMID:11585930 Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin HAT domain aa 1171-1621 2026-08-15 01:11:59 0
MEIS1B-MSCV
 
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RRID:Addgene_21022 MEIS1B Mus musculus Ampicillin PMID:10082572 Backbone Marker:Clontech; Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:11:59 0
CBP-Bromo-HAT-CH3-sp65
 
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RRID:Addgene_21026 CBP Mus musculus Ampicillin PMID:11585930 Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP65; Vector Types:in vitro TNT; Bacterial Resistance:Ampicillin Bromo+HAT+CH3 domains aa 1098-1877; E1239G mutation 2026-08-15 01:11:59 0
MSX2-pET
 
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1+ mentions
RRID:Addgene_21025 MSX2 Mus musculus Kanamycin PMID:9373945 Backbone Marker:Novagen; Backbone Size:5376; Vector Backbone:pET-28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:11:59 1
MEIS1B-pVP16
 
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RRID:Addgene_21020 MEIS1B Mus musculus Ampicillin Backbone Marker:Clontech; Backbone Size:3300; Vector Backbone:pVP16; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin VP16 activation domain fused to N-terminal 2026-08-15 01:11:59 0
pBABE-puro-mouse Oct4
 
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RRID:Addgene_21110 Oct4 Mus musculus Ampicillin PMID:19171782 Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:12:00 0
HOXD8-pSG5
 
Resource Report
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RRID:Addgene_21005 HOXD8 Mus musculus Ampicillin Backbone Marker:Stratagene; Backbone Size:4076; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:59 0

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