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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pRS415-BUD32
 
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RRID:Addgene_10991 BUD32 Saccharomyces cerevisiae Ampicillin Backbone Size:6021; Vector Backbone:pRS415; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin Wild type 2026-08-15 01:01:34 0
pRS314-hUD
 
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RRID:Addgene_11005 hUD recombination system Saccharomyces cerevisiae Ampicillin The repeats of HIS3 in this plasmid contain truncations of the HIS3 gene. The first repeat is a 3' end truncation that contains the promoter and the ORF from the ATG to the internal KpnI site (used for subcloning). The second repeat is a 5' truncation that contains from the internal NdeI site (used for subcloning) to the end of the gene. Recombination of the common DNA sequence between these repeats (approx. 430 nt internal to the HIS3 ORF) reconstitutes a functional gene. Both plasmids, containing the repeats in direct orientation (hUD) or inverted orientation (hUI), have been tested for recombination with positive result. Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin Direct repeat recombination system. Recombination reconstitutes a functional HIS3 gene 2026-08-15 01:01:36 0
pRS314-URA3
 
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1+ mentions
RRID:Addgene_11004 URA3 Saccharomyces cerevisiae Ampicillin Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin Wild type 2026-08-15 01:01:36 1
pRS314-hUI
 
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RRID:Addgene_11006 hUI recombination system Saccharomyces cerevisiae Ampicillin The repeats of HIS3 in this plasmid contain truncations of the HIS3 gene. The first repeat is a 3' end truncation that contains the promoter and the ORF from the ATG to the internal KpnI site (used for subcloning). The second repeat is a 5' truncation that contains from the internal NdeI site (used for subcloning) to the end of the gene. Recombination of the common DNA sequence between these repeats (approx. 430 nt internal to the HIS3 ORF) reconstitutes a functional gene. Both plasmids, containing the repeats in direct orientation (hUD) or inverted orientation (hUI), have been tested for recombination with positive result. Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin Inverted repeat recombination system. Recombination reconstitutes a functional HIS3 gene 2026-08-15 01:01:36 0
pRS415-SSM1
 
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RRID:Addgene_11003 SSM1 Saccharomyces cerevisiae Ampicillin Backbone Size:6021; Vector Backbone:pRS415; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin Wild type 2026-08-15 01:01:35 0
YEplac181-SSM1
 
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RRID:Addgene_11002 SSM1 Saccharomyces cerevisiae Ampicillin Backbone Size:5741; Vector Backbone:YEplac181; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin Wild type 2026-08-15 01:01:35 0
pYC6H-TRP1URA3
 
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RRID:Addgene_11009 Pgal1TRP1URA3 Saccharomyces cerevisiae Ampicillin Please note that Addgene's sequencing results identified mutations A160S and K173R in the URA3 sequence. These mutations were not intentional and the depositing scientist states that the plasmid was tested for complementation of URA and TRP phenotypes and for production of a single transcript. Backbone Size:3786; Vector Backbone:pYC6-CT/HIS3 hybrid; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin ORF-TRP1 fused to ORF-URA without stop codon 2026-08-15 01:01:36 0
pJO629
 
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RRID:Addgene_104342 pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 Saccharomyces cerevisiae Ampicillin PMID:29122887 The 3788 bp insert covers entire PRT module.For cloning of gene of interest, just use Asc I/Bam HI sites. Backbone Size:4967; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:41 0
pCSJ125
 
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RRID:Addgene_104344 MDH2 Saccharomyces cerevisiae Ampicillin PMID:28126757 Replace the Yap5 gene of pJO629 to MDH1 gene. Backbone Size:8017; Vector Backbone:pJO629; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:44 0
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
 
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1+ mentions
RRID:Addgene_104394 MCP-NLS-2xyeGFP Saccharomyces cerevisiae Ampicillin PMID:29131164 Insert expressed under the control of the CYC1 promoter and terminator Vector Backbone:YcpLac111; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin MCP fused to NLS(SV40) and 2x yeast codon optimized eGFP 2026-08-15 01:00:41 3
YIplac211-RPL13A-FKBPx4
 
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RRID:Addgene_104474 RPL13Ap Saccharomyces cerevisiae Ampicillin PMID:26709839 Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin includes the last 354 bp of RPL13A only 2026-08-15 01:00:42 0
YIplac211-RPL13A-FKBPx2
 
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RRID:Addgene_104473 RPL13Ap Saccharomyces cerevisiae Ampicillin PMID:26709839 Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin includes the last 354 bp of RPL13A only 2026-08-15 01:00:42 0
YIplac204-TC-OM45-FKBPx4
 
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RRID:Addgene_104472 OM45p Saccharomyces cerevisiae Ampicillin PMID:26709839 Backbone Size:3545; Vector Backbone:YIplac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:45 0
pILGFP4G
 
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RRID:Addgene_104502 ScPGM2 promoter-yEGFP Saccharomyces cerevisiae Ampicillin PMID:29301066 Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:43 0
YIplac211-iGFP-TLG1
 
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RRID:Addgene_105261 Tlg1 Saccharomyces cerevisiae Ampicillin PMID:29316441 Addgene's quality control identified G264D in URA3. The depositing lab states that this mutation should not affect selection. Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:53 0
YIplac211-ARF1-msGFP
 
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RRID:Addgene_105255 Arf1 Saccharomyces cerevisiae Ampicillin PMID:29316441 Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:53 0
YIplac211-CHC1-msGFPx3
 
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RRID:Addgene_105256 Chc1 portion Saccharomyces cerevisiae Ampicillin PMID:29316441 Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:49 0
YIplac211-APL5-mCherry2Bx6
 
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RRID:Addgene_105253 Apl5 Saccharomyces cerevisiae Ampicillin PMID:29316441 Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:49 0
YIplac211-APL6-msGFPx3
 
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RRID:Addgene_105254 Apl6 portion Saccharomyces cerevisiae Ampicillin PMID:29316441 Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:49 0
YIplac211-APL2-iGFPx6
 
Resource Report
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RRID:Addgene_105251 APL2 portion Saccharomyces cerevisiae Ampicillin PMID:29316441 Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:49 0

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