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Species: Mus musculus
Genetic Insert: Hsp68 promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2800; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12915490
Comments: For more information about this plasmid see attached information sheet.
Proper citation: RRID:Addgene_33351 Copy
Species: Mus musculus
Genetic Insert: Atoh1
Vector Backbone Description: Backbone Marker:Addgene plasmid #33336; Backbone Size:6575; Vector Backbone:pMSCV-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20516124
Proper citation: RRID:Addgene_33333 Copy
Species: Orthoreovirus T1L
Genetic Insert: S1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pBacPAK8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18005692
Proper citation: RRID:Addgene_33292 Copy
Species: Orthoreovirus T1L
Genetic Insert: S4
Vector Backbone Description: Backbone Marker:made by Yoshihiro Kawaoka; Vector Backbone:p3E5EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18005692
Proper citation: RRID:Addgene_33295 Copy
Species: Orthoreovirus T1L
Genetic Insert: S2
Vector Backbone Description: Backbone Marker:made by Yoshihiro Kawaoka; Vector Backbone:p3E5EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18005692
Proper citation: RRID:Addgene_33293 Copy
Species: Orthoreovirus T1L
Genetic Insert: L3
Vector Backbone Description: Backbone Marker:made by Yoshihiro Kawaoka; Vector Backbone:p3E5EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18005692
Proper citation: RRID:Addgene_33288 Copy
Species: Orthoreovirus T1L
Genetic Insert: M1
Vector Backbone Description: Backbone Marker:made by Yoshihiro Kawaoka; Vector Backbone:p3E5EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18005692
Proper citation: RRID:Addgene_33289 Copy
Species: Orthoreovirus T1L
Genetic Insert: L2
Vector Backbone Description: Backbone Marker:made by Yoshihiro Kawaoka; Vector Backbone:p3E5EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18005692
Proper citation: RRID:Addgene_33287 Copy
Species: Mus musculus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pRRLsin.PPT.hPGK.FLAG-CaMKKbeta-IRES.GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21669867
Comments: Please note that, according to NCBI numbering, the mutation is actually K194A.
This plasmid was also used in the following publication:
Cell Signal. 2011 Dec;23(12):2005-12. Epub 2011 Jul 23.
Proper citation: RRID:Addgene_33323 Copy
Species: Orthoreovirus T3D
Genetic Insert: S4
Vector Backbone Description: Backbone Marker:made by Yoshihiro Kawaoka; Vector Backbone:p3E5EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18005692
Proper citation: RRID:Addgene_33285 Copy
Species: Mus musculus
Genetic Insert: Malt1
Vector Backbone Description: Vector Backbone:pEF-FLAG-Strep; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21896478
Proper citation: RRID:Addgene_33315 Copy
Species: Homo sapiens
Genetic Insert: MIB2
Vector Backbone Description: Vector Backbone:pCX-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21896478
Proper citation: RRID:Addgene_33312 Copy
Species: Homo sapiens
Genetic Insert: MIB1
Vector Backbone Description: Vector Backbone:pCX-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21896478
Proper citation: RRID:Addgene_33317 Copy
Species: Drosophila melanogaster
Genetic Insert: CG1034
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34340 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518959
Proper citation: RRID:Addgene_34593 Copy
Genetic Insert: NLS-LSS-mKate2
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20212155
Comments: There is an 18bp deletion in Addgene's sequencing results relative to the full plasmid sequence (at position 2174-2192bp). This region is immediately after the LSS-mKate2 gene and is not critical for plasmid performance.
There is also a single nucleotide mismatch that results in an F22S change in the linker between the NLS and LSS-mKate2. It should not have any effect on the fusion performance.
Two other mismatches do not alter amino acid sequence.
Proper citation: RRID:Addgene_34586 Copy
Species: Mus musculus
Genetic Insert: Slug
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3xFlag-CMV-7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21610693
Proper citation: RRID:Addgene_34584 Copy
Genetic Insert: tRNA^Sep
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Tetracycline
Defining Citation: PMID:21868676
Proper citation: RRID:Addgene_34624 Copy
Species: Methanocaldococcuc maripaludis
Genetic Insert: SepRS
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4300; Vector Backbone:pKK223-3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21868676
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid.
Certain elements of this plasmid are covered under US patent 9,090,928 to Yale University
Proper citation: RRID:Addgene_34623 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Marker:Morganstern JP and Land H 1990; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518959
Proper citation: RRID:Addgene_34589 Copy
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