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Species: Mus musculus
Genetic Insert: HOXD4
Vector Backbone Description: Backbone Marker:Stratagene (originally); Backbone Size:4000; Vector Backbone:pBluescript derivative; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_21004 Copy
Species: Mus musculus
Genetic Insert: alpha myosin heavy chain promoter
Vector Backbone Description: Backbone Size:7233; Vector Backbone:SMPU; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19352491
Proper citation: RRID:Addgene_21231 Copy
Species: Mus musculus
Genetic Insert: PKC gamma
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_21236 Copy
Species: Mus musculus
Genetic Insert: PKC epsilon
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_21243 Copy
Species: Mus musculus
Genetic Insert: PKC eta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Comments: Osada, S., Mizuno, K., Saido, T.C., Akita, Y., Suzuki, K., Kuroki, T., and Ohno, S. 1990. A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin. The Journal of biological chemistry 265(36): 22434-22440.
Please note that Addgene was not able to completely confirm the truncation with quality control sequencing.
Proper citation: RRID:Addgene_21247 Copy
Species: Mus musculus
Genetic Insert: PKC eta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Comments: Osada, S., Mizuno, K., Saido, T.C., Akita, Y., Suzuki, K., Kuroki, T., and Ohno, S. 1990. A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin. The Journal of biological chemistry 265(36): 22434-22440.
Proper citation: RRID:Addgene_21245 Copy
Species: Mus musculus
Genetic Insert: PKC epsilon
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_21240 Copy
Species: Mus musculus
Genetic Insert: alpha myosin heavy chain promoter
Vector Backbone Description: Backbone Size:8400; Vector Backbone:SIN18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19352491
Proper citation: RRID:Addgene_21229 Copy
Species: Mus musculus
Genetic Insert: alpha myosin heavy chain promoter
Vector Backbone Description: Backbone Size:7338; Vector Backbone:SMPU; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19352491
Comments: There is an extra C in the promoter sequence when compared to the available reference sequence in NCBI. The mismatch is due to older sequencing artifact of an erroneous insertion of a "C" adjacent to "GGG". The construct should be correct.
Proper citation: RRID:Addgene_21228 Copy
Species: Mus musculus
Genetic Insert: Axin2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:For riboprobe synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11809808
Comments: Tong DNA #94. The DNA is a HinDIII fragment of mouse Axin2 (nucleotides 1-2397) in pBluescript-KS. The antisense and sense Axin2 riboprobes were made by linearizing this plasmid with NotI or SalI and transcribing with T3 or T7 RNA polymerase, respectively. List of DNAs says " ~2 kb DIII fragment of Axin2 (1-2397) in pBS-KS”.
Proper citation: RRID:Addgene_21277 Copy
Species: Mus musculus
Genetic Insert: Axin2 promoter
Vector Backbone Description: Backbone Marker:Clontech (discontinued); Backbone Size:4300; Vector Backbone:d2EGFP-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11809808
Comments: The 5.6-kb DNA fragment was excised from Axin2-Luciferase using KpnI and HindIII (the HindIII site was filled in with Klenow DNA polymerase) and cloned into the KpnI- and SmaI-digested pd2EGFP-1 vector.
Proper citation: RRID:Addgene_21276 Copy
Species: Mus musculus
Genetic Insert: Axin2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11809808
Comments: A 5.6-kb DNA fragment containing the Axin2 promoter, exon 1, intron 1, and part of exon 2 was subcloned from a mouse BAC clone and sequenced. In Author's map, exons are shown as gray boxes, and the sequence is numbered with bp 1 corresponding to the start of the cDNA sequence. Eight conserved Tcf/LEF
binding sites (underlined and designated T1 to T8) were identified.
Proper citation: RRID:Addgene_21275 Copy
Species: Mus musculus
Genetic Insert: Axin2
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11809808
Proper citation: RRID:Addgene_21279 Copy
Species: Mus musculus
Genetic Insert: Axin2
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11809808
Comments: Full-length Axin2 cDNA. Tong clone #99.
Proper citation: RRID:Addgene_21278 Copy
Species: Mus musculus
Genetic Insert: Int3
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pLNC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9576833
Proper citation: RRID:Addgene_21329 Copy
Species: Mus musculus
Genetic Insert: Axin
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9230313
Proper citation: RRID:Addgene_21287 Copy
Species: Mus musculus
Genetic Insert: Notch4
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBSKS; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8681805
Proper citation: RRID:Addgene_21325 Copy
Species: Mus musculus
Genetic Insert: Krox20-Myelin Specific Enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19179536
Comments: Plasmid 21261: Krox20-MSE8 pGL3-TATA sequence match with genomic sequences on Chromosome 10 upstream of Egr2. It's an enhancer of Egr2 localized ~35 kb downstream of the defined genomic sequence of Egr2. If comparing the genomic location of plasmid 21261 (Chromosome 10: 9367948-9368511) and plasmid 21260 (Chromosome 10: 9331837-9332418), one can see that it's approximately 35 kb apart.
Proper citation: RRID:Addgene_21261 Copy
Species: Mus musculus
Genetic Insert: Krox20 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19179536
Comments: There is a T to C mismatch with Krox20. The single mis-match of the nucleotide is likely to reflect the polymorphism of different mouse strain used in the cloning.
Proper citation: RRID:Addgene_21260 Copy
Species: Mus musculus
Genetic Insert: Notch4
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBSKS; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9576833
Proper citation: RRID:Addgene_21470 Copy
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