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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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YFP(159-238)Stop in pcDNA1/amp Resource Report Resource Website |
RRID:Addgene_60891 | YFP(159-238)Stop | Aequorea victoria | Ampicillin | Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNA1/amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:37 | 0 | |||
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pcDNA-Flag-Tet3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_60940 | Ten-Eleven Translocation 3 | Mus musculus | Ampicillin | PMID:24412366 | Backbone Size:6000; Vector Backbone:pcDNA-flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 4 | ||
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pJET1.2-STOP-dsRed Resource Report Resource Website 1+ mentions |
RRID:Addgene_60944 | dsRED | Other | Ampicillin | PMID:25324299 | The plasmid contains an IS4-like element that does not interfere with function as described in the associated publication. | Backbone Marker:Thermo Scientific; Backbone Size:2974; Vector Backbone:pJET1.2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 1 | |
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p3XFlag-CAPN2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_60942 | Calpain 2 | Homo sapiens | Ampicillin | PMID:24412366 | Backbone Marker:sigma; Backbone Size:6310; Vector Backbone:p3xFlag-CMV-14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:37 | 4 | ||
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p3xP3-EGFP.vas-int.NLS Resource Report Resource Website 1+ mentions |
RRID:Addgene_60948 | vas-phiC31 integrase.NLS | Other | Ampicillin | PMID:25324299 | Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:RMCE; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:37 | 4 | ||
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pBS-GGAC-ATGC Resource Report Resource Website 1+ mentions |
RRID:Addgene_60949 | Ampicillin | PMID:25324299 | Backbone Marker:Stratagene; Backbone Size:2905; Vector Backbone:pBluescript II; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 2 | ||||
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pBS-CGGA-GTGC Resource Report Resource Website |
RRID:Addgene_60950 | Ampicillin | PMID:25324299 | Backbone Marker:Stratagene; Backbone Size:2905; Vector Backbone:pBluescript II; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:37 | 0 | ||||
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pSwith-tdTomato Resource Report Resource Website |
RRID:Addgene_60953 | tdTomato | Ampicillin | PMID:24503648 | Backbone Marker:GeneArt; Backbone Size:2450; Vector Backbone:pMXs; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:37 | 0 | |||
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PX551 Resource Report Resource Website 10+ mentions |
RRID:Addgene_60957 | SpCas9 | Synthetic | Ampicillin | PMID:25326897 | Backbone Size:2905; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:37 | 49 | ||
|
GOMO-GFP-LUC Resource Report Resource Website 1+ mentions |
RRID:Addgene_60976 | GFP-Luciferase fusion | Synthetic | Ampicillin | PMID:24755823 | Backbone Size:2877; Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 3 | ||
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Ratiometric FPX sensor for ERK kinase activity Resource Report Resource Website 1+ mentions |
RRID:Addgene_60974 | ddRFP A-WW domain-ERK substrate-ddFP B | Synthetic | Ampicillin | PMID:25622108 | Backbone Size:5500; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 1 | ||
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pMTB-Multibow-fR Resource Report Resource Website |
RRID:Addgene_60984 | mKate2 | Synthetic | Ampicillin | PMID:26010570 | Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region. | Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 0 | |
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phMYT1L-N106 Resource Report Resource Website 1+ mentions |
RRID:Addgene_60861 | MYT1L | Homo sapiens | Ampicillin | PMID:25374357 | Backbone Marker:Homemade; Backbone Size:8465; Vector Backbone:N106; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:37 | 1 | ||
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pMTB-Multibow-O Resource Report Resource Website |
RRID:Addgene_60982 | mKO | Synthetic | Ampicillin | PMID:26010570 | Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region. | Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 0 | |
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pXCas9H840A Resource Report Resource Website 1+ mentions |
RRID:Addgene_60900 | Ampicillin | PMID:27030102 | The gRNA cloning strategy is exactly the same as for pX330/pX335. | Backbone Size:8506; Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:37 | 1 | |||
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pMTB-Multibow-mY Resource Report Resource Website |
RRID:Addgene_60988 | citrine | Synthetic | Ampicillin | PMID:26010570 | Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region. | Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 0 | |
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pMTB-Multibow-mB Resource Report Resource Website |
RRID:Addgene_60985 | EBFP2 | Synthetic | Ampicillin | PMID:26010570 | Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region. | Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 0 | |
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pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS Resource Report Resource Website 50+ mentions |
RRID:Addgene_60904 | scFv-GCN4 | Ampicillin | PMID:25307933 | For more information, visit https://valelab4.ucsf.edu/external/research/suntag.html Note this plasmid was used with the CRISPRa library, as described in Gilbert et al. Cell 2014 PubMed ID: 25307932. | Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:37 | 59 | ||
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pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP Resource Report Resource Website 10+ mentions |
RRID:Addgene_60903 | Cas9 dead | Ampicillin | PMID:25307933 | For more information, visit https://valelab4.ucsf.edu/external/research/suntag.html Note this plasmid was used with the CRISPRa library, as described in Gilbert et al. Cell 2014 PubMed ID: 25307932. | Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:37 | 49 | ||
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CIP2Aprom1802bp-pGL4.10Luc Resource Report Resource Website |
RRID:Addgene_60868 | 1802 bp promoter fragment of Cancerous Inhibitor of PP2A | Homo sapiens | Ampicillin | PMID:21445343 | Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function. | Backbone Marker:Promega; Backbone Size:4242; Vector Backbone:pGL4.10(luc2); Vector Types:Luciferase, Promoterless; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:37 | 0 |
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