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Species: E. coli
Genetic Insert: PLitR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2ab parts are single prmoters in the CTK system
Proper citation: RRID:Addgene_248371 Copy
Species: Other
Genetic Insert: dropout part, lv0, pos 4, mScarlet-I
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249996 Copy
Species: Other
Genetic Insert: dropout part, lv0, pos 5, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249997 Copy
Species: Other
Genetic Insert: CDS, lv 0, lux operon
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249912 Copy
Species: Aequorea victoria
Genetic Insert: Protein A
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252975 Copy
Species: Synthetic
Genetic Insert: FLAG-tagged 649 stalk display anchor
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_252979 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET15
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253016 Copy
Species: Synthetic
Genetic Insert: pNG2 ORI plus NGS barcode 22, chloramphenicol resistance cassette, mScarlet-I cassette, oriT
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Please note that this plasmid may run as a dimer or higher multimer.
Proper citation: RRID:Addgene_251860 Copy
Species: Synthetic
Genetic Insert: arsR, menD
Vector Backbone Description: Vector Backbone:p15A ori_cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://doi.org/10.1101/2025.09.24.678173 for bioRxiv preprint.
Proper citation: RRID:Addgene_252937 Copy
Species: Trichoderma reesei
Genetic Insert: Hydrophobin1 (HFB1)
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253013 Copy
Species: Unspecified
Genetic Insert: AID*-3HA-mNeonGreen
Vector Backbone Description: Vector Backbone:pBluescript II SK+; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_253584 Copy
Species: Synthetic
Genetic Insert: 5x 2EBS-S10 (A1-A2)
Vector Backbone Description: Vector Backbone:pUPD2; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38379432
Proper citation: RRID:Addgene_254056 Copy
Vector Backbone Description: Vector Backbone:DP6; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41980963
Comments: Contains CcdB Dropout Cassette. Take care to grow on a resistant strain for plasmid amplification. For ease of use when constructing oligo libraries for RAMPhaGE Libraries, please find the corresponding RAMPhaGE Oligo Tool Colab Notebook: https://colab.research.google.com/github/Niopek-Lab/POGO_PANCE/blob/main/scripts/RAMPhaGE_Oligo_Tool.ipynb.
Please visit https://doi.org/10.1101/2025.06.12.659331 for bioRxiv preprint.
Proper citation: RRID:Addgene_242157 Copy
Genetic Insert: SRTF1-responsive promoter
Vector Backbone Description: Vector Backbone:pSELIS; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: This plasmid contains two SRTF1-responsive promoter sequences, one upstream of sfGFP and one upstream of a cI Repressor.
Proper citation: RRID:Addgene_246681 Copy
Species: Geobacillus stearothermophilus (geocas9)
Genetic Insert: Plac/tet_GeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37833505
Proper citation: RRID:Addgene_207567 Copy
Species: Geobacillus thermodenitrificans T12 (thermocas9)
Genetic Insert: Plac/tet_dThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37833505
Proper citation: RRID:Addgene_207568 Copy
Species: Acidibacillus sulfuroxidans, Escherichia coli
Genetic Insert: Acidibacillus sulfuroxidans Cas12f1-ABE
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41399500
Proper citation: RRID:Addgene_220995 Copy
Genetic Insert: EcDRT3 (Drt3a + Drt3b + ncRNA)
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41990131
Proper citation: RRID:Addgene_255316 Copy
Species: Synthetic
Genetic Insert: G1PylRSMIFAF
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253672 Copy
Species: E. coli
Genetic Insert: L3S3P11
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 4 pasts are terminators in the CTK system
Please note: This plasmid contains a C nucleotide insertion in L3S3P11. This insertion is not known to impact plasmid function.
Proper citation: RRID:Addgene_248472 Copy
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