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Species: Saccharomyces cerevisiae
Genetic Insert: SOH1
Vector Backbone Description: Backbone Size:6021; Vector Backbone:pRS415; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15082542
Proper citation: RRID:Addgene_8622 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Nop1pr-GFP11-mCherry-PUS1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27831485
Proper citation: RRID:Addgene_86413 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HHT2
Vector Backbone Description: Backbone Marker:Akash Gunjan, Addgene Plasmid 86470; Vector Backbone:pYES2-HTH; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19578373
Proper citation: RRID:Addgene_86482 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mTurquoise2
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pFA6-link-CaURA3MX; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26371506
Comments: use F5/R3 primers
Proper citation: RRID:Addgene_86424 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1
Vector Backbone Description: Backbone Marker:Kuo et al, Mol Cell Biol. 1983 October; 3(10): 1730; Backbone Size:0; Vector Backbone:YCp50; Vector Types:Yeast Expression, Centromeric; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3540600
Comments: The TUB1 centromere plasmid was constructed by ligating a TUB1 fragment from the SphI site (1.1 kilobases before the start codon) to a BglII site (0.5 kb beyond the stop codon) in place of the small SphI-to-BamHI fragment of YCp50. For more information on YCp50, including map, search for YCp50 in Addgene's vector db.
Proper citation: RRID:Addgene_8667 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1
Vector Backbone Description: Backbone Marker:Bolivar et al., Gene. 1977;2(2):95-113.; Backbone Size:0; Vector Backbone:pBR322; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3540600
Comments: For more information on pBR322, including a map, search for pBR322 in Addgene's vector db.
Proper citation: RRID:Addgene_8666 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1 upstream of LEU2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pUC119; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10793159
Comments: TUB1 fragment (SphI-SacI) from pRB306 inserted into SphI-SacI-cut polylinker of pUC119.
Then, BamHI-SalI fragment from pJJ282 (LEU2 gene) Klenow filled and inserted into the HpaI site, downstream of TUB1; LEU2 is oriented in the same direction as TUB1. For information on pUC119, search for pUC119 in Addgene's vector db.
Proper citation: RRID:Addgene_8669 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1-E255A under GAL1 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pTS210; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11739794
Comments: PCRed TUB1-E255A products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.
Proper citation: RRID:Addgene_8674 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1 with LYS2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pUC119; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10793159
Comments: TUB1 fragment (SphI-SacI) from pRB306 inserted into SphI-SacI-cut polylinker of pUC119. Then, PvuII fragment from pRS317 containing LYS2 gene inserted into HpaI site; LYS2 is oriented in the same direction as TUB1. For more information on pUC119, search for pUC119 in Addgene's vector db.
Proper citation: RRID:Addgene_8671 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GFP::TUB1 under GAL1 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pTS408; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11739794
Comments: TUB1 wildtype DNA were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS408. pTS408 was constructed by PCR amplifying GFP with BamHI and BglII sites and by inserting this into the BamHI site of pTS210, creating a plasmid with the polylinker downstream of GFP to create NH2-terminal GFP fusions.The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.
Proper citation: RRID:Addgene_8677 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87280 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87281 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87287 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87286 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87276 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87275 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87278 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87279 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87266 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87269 Copy
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