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Species: Saccharomyces cerevisiae
Genetic Insert: Artificial Transcription Factor (McIsaac et al, 2013)
Vector Backbone Description: Vector Backbone:pMN10 (McIsaac et al, 2013); Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31249062
Proper citation: RRID:Addgene_113335 Copy
Species: Homo sapiens
Genetic Insert: NMHC II-A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11029059
Comments: NMHC II-A can be released by HindIII/SpeI digestion.
Please note that the original pEGFP-C3 vector was modified so that the tetracyclin regulated promoter was replaced by a CMV promoter.
Proper citation: RRID:Addgene_11347 Copy
Species: Homo sapiens
Genetic Insert: UBE4B
Vector Backbone Description: Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29599191
Proper citation: RRID:Addgene_113499 Copy
Species: Synthetic
Genetic Insert: 158_33121123_1
Vector Backbone Description: Backbone Size:5222; Vector Backbone:pGC4750; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30247489
Proper citation: RRID:Addgene_113408 Copy
Species: Homo sapiens
Genetic Insert: Kcnmb2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:ECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_113562 Copy
Species: Homo sapiens
Genetic Insert: Kcnmb4
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_113560 Copy
Species: Homo sapiens
Genetic Insert: Kcnmb4
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:ECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_113564 Copy
Genetic Insert: Hybrid Promoter : Para_tet_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Proper citation: RRID:Addgene_113601 Copy
Genetic Insert: Hybrid Promoter : Para_lac_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Proper citation: RRID:Addgene_113606 Copy
Species: Other
Genetic Insert: TfRΔEctoR1-mRFP-GFPnb
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29590595
Proper citation: RRID:Addgene_113555 Copy
Species: Homo sapiens
Genetic Insert: Kcnmb3
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_113559 Copy
Species: Synthetic
Genetic Insert: mRFP1-linker-sfGFP
Vector Backbone Description: Vector Backbone:pQE-80L. ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27648665
Comments: https://benchling.com/s/IF2YcdLA
Proper citation: RRID:Addgene_113642 Copy
Genetic Insert: Hybrid Promoter : Plas_lac_tet_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Proper citation: RRID:Addgene_113595 Copy
Genetic Insert: Hybrid Promoter : Plux_lac_tet_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Comments: Note: Plasmid contains a tet operator instead of a lac operator between rop and myc. This change is not known to affect plasmid function.
Proper citation: RRID:Addgene_113594 Copy
Genetic Insert: Hybrid Promoter : Prhl_lac_tet_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Comments: Note: Plasmid contains a tet operator instead of a lac operator between rop and myc. This change is not known to affect plasmid function.
Proper citation: RRID:Addgene_113593 Copy
Species: Other
Genetic Insert: mKaxxte2.5
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30804405
Comments: One clones a fragment of DNA sequence at the MCS, then uses a targeted nuclease, such as Cas9 to trigger a double-strand break. This is then repaired by homologous recombination resulting in restoration of an intact mKate2.5 fluorescent protein (cloned from Addgene #54828). It can be used to test Cas9 gRNA efficiency, as it was in the paper, or HR-capacity in different cells.
Proper citation: RRID:Addgene_113630 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ulp1_R4
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29976752
Proper citation: RRID:Addgene_113672 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ulp1_R1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29976752
Proper citation: RRID:Addgene_113669 Copy
Genetic Insert: CBF (notch dependent promoter)
Vector Backbone Description: Backbone Size:4403; Vector Backbone:pMuLE ENTR SV40 tdTomato L3-L2; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31860685
Proper citation: RRID:Addgene_113708 Copy
Species: Other
Genetic Insert: mEGFP-mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert C-terminal mEGFP-mEGFP at your locus of interest.
Proper citation: RRID:Addgene_113750 Copy
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