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Species: Homo sapiens
Genetic Insert: ERK1
Vector Backbone Description: Vector Backbone:NpT7-5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8444886
Proper citation: RRID:Addgene_39229 Copy
Species: Homo sapiens
Genetic Insert: HERC2 HECT Domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21193487
Comments: HECT domain active site point mutation (C4762A substitution)
Proper citation: RRID:Addgene_39228 Copy
Species: Homo sapiens
Genetic Insert: scFv-Fc-fusion specific for Notch1 (clone B9)
Vector Backbone Description: Backbone Marker:McCafferty lab unpublished; Vector Backbone:pBIOCAM5-3F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22842086
Comments: Binds to both mouse mNRR1 and human hNRR1 but not NRR2.
Proper citation: RRID:Addgene_39344 Copy
Species: Homo sapiens
Genetic Insert: scFv-Fc-fusion specific for Desmin (clone D7)
Vector Backbone Description: Backbone Marker:McCafferty lab unpublished; Vector Backbone:pBIOCAM5-3F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18047641
Proper citation: RRID:Addgene_39346 Copy
Species: Homo sapiens
Genetic Insert: scFv specific for Jagged (clone D5)
Vector Backbone Description: Vector Backbone:pBIOCAM5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18047641
Proper citation: RRID:Addgene_39345 Copy
Species: Homo sapiens
Genetic Insert: Myocilin wild-type
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19959812
Proper citation: RRID:Addgene_39326 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Do not cut out EB1 using EcoR1 as there is an internal EcoR1 site in the EB1 sequence.
Proper citation: RRID:Addgene_39324 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39307 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39303 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39306 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: This mutant does not bind microtubule tips.
Proper citation: RRID:Addgene_39300 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39302 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39301 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-25-Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCGGCGAGCTGCACG
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2kb and 5.8kb
Proper citation: RRID:Addgene_39407 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-25-Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32285); Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCAAGATCCGCCACA
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2kb and 5.8kb
Proper citation: RRID:Addgene_39406 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-23-Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCGTCCTTGAAGAAG
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2kb and 5.8kb
Proper citation: RRID:Addgene_39403 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-24-Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TTGCCGTCCTCCTTG
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2kb and 5.8kb
Proper citation: RRID:Addgene_39405 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-06-Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TGCCACCTACG
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 1.6kb and 5.8kb
Proper citation: RRID:Addgene_39368 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-05-Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TAGGTGGCATC
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 1.6kb and 5.8kb
Proper citation: RRID:Addgene_39367 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-22-Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TGGTGCCCATCCTGG
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2kb and 5.8kb
Proper citation: RRID:Addgene_39400 Copy
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