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Species: Homo sapiens
Genetic Insert: dynamin 1
Vector Backbone Description: Vector Backbone:pUHD10-3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7559787
Proper citation: RRID:Addgene_34688 Copy
Species: Homo sapiens
Genetic Insert: dynamin 1ba
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_34682 Copy
Species: Homo sapiens
Genetic Insert: dynamin 1ba
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15004222
Proper citation: RRID:Addgene_34681 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:Derived from pBluescript II SK+; Vector Types:Hydra Expression; Bacterial Resistance:Ampicillin
Comments: https://www.ncbi.nlm.nih.gov/nuccore/158426709
Proper citation: RRID:Addgene_34794 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript; Vector Types:Bacterial Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12399591
Comments: Use HT115 bacteria for experiments--Grow O/N on LB supplemented with carbenicillin (100ug/ml) & tetracyclin (12.5ug/ml) and pick single colony for liquid culture.
Proper citation: RRID:Addgene_34833 Copy
Species: Homo sapiens
Genetic Insert: CD68 promoter/enhancer -680/+140
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11907098
Comments: The CD68 promoter/enhancer in this construct consists of ~680bp 5' of the ATG and the 83bp first intron of human CD68.
Please note that Addgene's sequencing results found a single nucleotide deletion at bp# 440 when compared to the full plasmid sequence provided by the depositor. The depositing scientist states that it is probably a polymorphism in the promoter and is not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_34837 Copy
Species: Mus musculus
Genetic Insert: GATA3
Vector Backbone Description: Backbone Marker:Hergen Spits and coworkers; Backbone Size:12443; Vector Backbone:LZRSpBMN-linker-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12871641
Proper citation: RRID:Addgene_34836 Copy
Species: E. Coli
Genetic Insert: LplA W37V
Vector Backbone Description: Backbone Marker:Gift from John Cronan; Backbone Size:4800; Vector Backbone:pYFJ16; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20534555
Proper citation: RRID:Addgene_34838 Copy
Species: Homo sapiens
Genetic Insert: TFAM
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037171
Proper citation: RRID:Addgene_34705 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Created by Köster and Fraser; Vector Backbone:act-GVP-UG; Vector Types:Xenopus expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22113462
Comments: The Sox2/Oct3-4 enhancer elements and a minimal promoter from the murine FGF4 were subcloned from the plasmid described in Figure 1 of Ambrosetti et al. (2000). The fragment contains six tandem repeats of the 35-bp fragment containing the Sox2/Oct3-4 heterodimer transcription factor binding domain (bd) followed by a 165-bp minimal FGF4 promoter (mFGF4). This plasmid was a gift of Claudio Basilico (New York University). To increase the FP signal, the Sox2/Oct 3-4-mFGF regulatory fragment was moved to the “act-GVP-UG” concatenated plasmid created by Köster and Fraser (2001), which was adapted from the Drosophila Gal4-UAS system (Brand and Perrimon,1993) and shown to function in Xenopus (Chae et al.,2002; Hartley et al.,2002). We replaced the minimal promoters of the act-GVP-UG plasmid with our Sox2/Oct3-mFGF regulatory fragment to control the expression of the transcriptional activator, Gal4-VP16, which in turn drives the expression of genes controlled by the upstream activating sequence (UAS).
The final expression vector (abbreviated as pSox2-bd::FP) contains: 6 repeats of the Sox2/Oct3-4 transcription factor binding domain, mFGF4, Gal4-VP16, a polyadenylation site, 14 repeats of UAS, mFGF4, and eGFP (ClonTech, Palo Alto, CA).
Sequencing with EGFP-N identified 1nt deletion at position 1733, 1 nt insertion at position 1214, 2 nt insertion at 1557 compared to the depositor provided sequence. These mutations are all in non-coding regions and have been shown by the depositing lab to not effect the function of the plasmid.
Proper citation: RRID:Addgene_34703 Copy
Species: Homo sapiens
Genetic Insert: NRF1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7000; Vector Backbone:pMSCV-hyg; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20385086
Proper citation: RRID:Addgene_34707 Copy
Species: Homo sapiens
Genetic Insert: cytokine induced apoptosis inhibitor 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_34811 Copy
Species: Caenorhabditis elegans
Genetic Insert: Peft-3 GFP H2B tbb-2 UTR
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Comments: We would like to thank Andrew Wood and Barbara Meyer for sending us the Peft-3 promoter and pointing out how strong the promoter is.
Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_34877 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mos1 transpoase
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_34874 Copy
Species: Mus musculus
Genetic Insert: postsynaptic mGRASP
Vector Backbone Description: Backbone Size:5000; Vector Backbone:paavCAG; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22138823
Proper citation: RRID:Addgene_34913 Copy
Genetic Insert: SB100X transposase
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19412179
Comments: The Sleeping Beauty transposon system consists of two components: the transposon vector and the transposase vector. The transposon vector (pT4/HB, Addgene #108352) contains binding sites flanking the GOI. And the transposase vector (pCMV(CAT)T7-SB100, Addgene #34879) encodes for the enzyme mediating excision and integration of the GOI.
Proper citation: RRID:Addgene_34879 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: Dual resistance (PuroR-NeoR) vector for drug selection following biolistic bombardment in worms.
This vector contains a Pmyo-2::mCherry::myo-2_3'UTR pharyngeal marker on the backbone. Worm strains generated with vector can be crossed with strains generated with other visual markers such as in pBCN41-R4R3 which has a green fluorescent pharyngeal marker.
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34915 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi5605 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34866 Copy
Species: Homo sapiens
Genetic Insert: PIMT
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET30b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18318686
Proper citation: RRID:Addgene_34852 Copy
Species: Mus musculus
Genetic Insert: Fos promoter/first intron -764/+918
Vector Backbone Description: Vector Backbone:pBS; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17761885
Proper citation: RRID:Addgene_34856 Copy
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