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Species: Other
Genetic Insert: mRuby2-mRuby2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mRuby2-mRuby2 at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113753 Copy
Species: Other
Genetic Insert: mRuby2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mRuby2 at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113752 Copy
Species: Other
Genetic Insert: mEGFP-mEGFP-mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert C-terminal mEGFP-mEGFP-mEGFP at your locus of interest.
Proper citation: RRID:Addgene_113751 Copy
Species: Other
Genetic Insert: mRuby2-mRuby2-mRuby2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert C-terminal mRuby2-mRuby2-mRuby2 at your locus of interest.
Proper citation: RRID:Addgene_113757 Copy
Species: Other
Genetic Insert: mRuby2-mRuby2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert C-terminal mRuby2-mRuby2 at your locus of interest.
Proper citation: RRID:Addgene_113756 Copy
Species: Other
Genetic Insert: PpU6P::sgRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2551; Vector Backbone:pDONR221-P5P4; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Proper citation: RRID:Addgene_113741 Copy
Species: Other
Genetic Insert: mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mEGFP at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113746 Copy
Species: Synthetic
Genetic Insert: stop cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to generate a premature stop codon at your locus of interest. Also contains a multiple cloning site for easy genotyping. This plasmid contains multiple M13F and M13R sites, due to cloning the MCS of pBluescriptSK+.
QC note: This plasmid dimerizes during growth in bacteria, which can result in digest and Sanger sequencing results that differ from the full plasmid sequence of the monomer. The depositing lab has confirmed that dimerization does not affect plasmid function.
Proper citation: RRID:Addgene_113745 Copy
Species: Other
Genetic Insert: mEGFP-mEGFP-mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mEGFP-mEGFP-mEGFP at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113748 Copy
Species: Other
Genetic Insert: PpU6P::sgRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2538; Vector Backbone:pDONR221-P1P2; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Proper citation: RRID:Addgene_113735 Copy
Species: Other
Genetic Insert: PpU6P::sgRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2604; Vector Backbone:pDONR221-P1P5r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Proper citation: RRID:Addgene_113737 Copy
Species: Nicotiana benthamiana
Genetic Insert: Niben101Scf05536g01001.1
Vector Backbone Description: Vector Backbone:pGWB14; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_126298 Copy
Species: Nicotiana benthamiana
Genetic Insert: Niben101Scf04664g01002.1
Vector Backbone Description: Vector Backbone:pGWB14; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_126299 Copy
Species: Nicotiana benthamiana
Genetic Insert: Niben101Scf04706g01012.1
Vector Backbone Description: Vector Backbone:pGWB14; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_126332 Copy
Species: Nicotiana benthamiana
Genetic Insert: Niben101Scf03049g06001.1
Vector Backbone Description: Vector Backbone:pGWB14; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Comments: The insert contains a native intron and will be processed to a mature mRNA when expressed in vivo.
Proper citation: RRID:Addgene_126338 Copy
Species: Nicotiana benthamiana
Genetic Insert: Niben101Scf02025g02002.1
Vector Backbone Description: Vector Backbone:pGWB14; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_126336 Copy
Species: Nicotiana benthamiana
Genetic Insert: Niben101Scf02589g01004.1
Vector Backbone Description: Vector Backbone:pGWB14; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Comments: The insert contains a native intron and will be processed to a mature mRNA when expressed in vivo.
Proper citation: RRID:Addgene_126339 Copy
Species: Nicotiana benthamiana
Genetic Insert: Niben101Scf08001g00002.1
Vector Backbone Description: Vector Backbone:pGWB14; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_126292 Copy
Species: Nicotiana benthamiana
Genetic Insert: Niben101Scf00080g05006.1
Vector Backbone Description: Vector Backbone:pGWB14; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_126293 Copy
Species: Nicotiana benthamiana
Genetic Insert: Niben101Scf09489g00001.1
Vector Backbone Description: Vector Backbone:pGWB14; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_126290 Copy
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