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Species: Rattus norvegicus
Genetic Insert: PKC beta 1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_16380 Copy
Species: Homo sapiens
Genetic Insert: HSP70
Vector Backbone Description: Backbone Marker:Addgene (#85042); Backbone Size:4000; Vector Backbone:pmScarlet_C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32472079
Proper citation: RRID:Addgene_163790 Copy
Vector Backbone Description: Vector Backbone:AAV2 ITR vector; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33721016
Proper citation: RRID:Addgene_163793 Copy
Vector Backbone Description: Vector Backbone:AAV2 ITR vector; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33721016
Proper citation: RRID:Addgene_163796 Copy
Species: Mus musculus
Genetic Insert: PKC beta II
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_16384 Copy
Vector Backbone Description: Backbone Marker:(Addgene Plasmid 163631); Backbone Size:8631; Vector Backbone:plRL2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34297925
Proper citation: RRID:Addgene_163954 Copy
Species: Homo sapiens
Genetic Insert: PKG1 beta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12492113
Comments: The PKG I beta insert aligns with the listed reference sequence after amino acid 90, contains an EF insertion after the starting methionine and I537V mutation.
Proper citation: RRID:Addgene_16394 Copy
Vector Backbone Description: Backbone Size:9220; Vector Backbone:pAdTrack-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9482916
Comments: Vector pAdTrack-CMV is used for expression of transgenes under a CMV promoter when a GFP tracer is desired.
Max insert size = 5.0kb with pAdEasy1 and 7.7kb with pAdEasy2.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
Proper citation: RRID:Addgene_16405 Copy
Vector Backbone Description: Backbone Size:8320; Vector Backbone:pAdTrack; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9482916
Comments: Vector pAdTrack is used for expression of transgenes under a chosen (non-CMV) promoter when a GFP tracer is desired.
Max insert size = 5.9kb with pAdEasy1 and 8.6kb with pAdEasy2.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
Proper citation: RRID:Addgene_16404 Copy
Vector Backbone Description: Backbone Size:7452; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9482916
Comments: Vector pShuttle-CMV is used for expression of transgenes under a CMV promoter when no GFP tracer is desired.
Max insert size = 6.6kb with pAdEasy1 and 9.3kb with pAdEasy2.
See AdEasy Protocol linked in the supplemental documents section above for more information.
NOTE: There are discrepancies between the Addgene quality control sequence and the assembled sequence from the Vogelstein lab. These differences are not known to affect function.
Proper citation: RRID:Addgene_16403 Copy
Vector Backbone Description: Backbone Size:6736; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9482916
Comments: Vector pShuttle is used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.
Max insert size = 7.5kb with pAdEasy1 and 10.2kb with pAdEasy2.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
Proper citation: RRID:Addgene_16402 Copy
Species: Homo sapiens
Genetic Insert: Split GFP Mt-ER Short P2A
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247103
Proper citation: RRID:Addgene_164108 Copy
Species: Synthetic
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Size:7700; Vector Backbone:lentiCas9-Blast plasmid (Addgene #52962); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33539780
Proper citation: RRID:Addgene_164135 Copy
Species: Homo sapiens
Genetic Insert: SacI ML GFP Strand 11 Long
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29229997
Proper citation: RRID:Addgene_164120 Copy
Vector Backbone Description: Backbone Size:10183; Vector Backbone:lentiGuide-Puro (Addgene #52963); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33539780
Proper citation: RRID:Addgene_164124 Copy
Genetic Insert: NES-PhoCl2c-mCherry
Vector Backbone Description: Backbone Size:5350; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34349937
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.10.419556v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_164037 Copy
Species: Synthetic
Genetic Insert: NES-mCardinal-NLS
Vector Backbone Description: Backbone Size:5350; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34349937
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.10.419556v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_164052 Copy
Species: Homo sapiens
Genetic Insert: RPA70
Vector Backbone Description: Backbone Size:7879; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164231 Copy
Species: Homo sapiens
Genetic Insert: GLI
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2832761
Comments: GlI K12 contains the entire coding region of GLI (-79 to 3522) cloned into the EcoRI site of pBluescript KS. The GLI cDNA contains an internal EcoRI site so the insert should be excised by cleavage with HindIII (at 5' end of Gli cDNA) and XbaI (at 3' end of Gli cDNA).
Proper citation: RRID:Addgene_16419 Copy
Species: Homo sapiens
Genetic Insert: ACE2 extracellular domain
Vector Backbone Description: Backbone Marker:Addgene plasmids 12154; Vector Backbone:pCSCG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33103998
Proper citation: RRID:Addgene_164222 Copy
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