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Genetic Insert: PSmOrange2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22900938
Comments: https://www.ncbi.nlm.nih.gov/nuccore/JQ392581 There are D26G and V119I amino acid changes in Addgene's quality control sequence for H2B. It does not alter the function of this plasmid.
Proper citation: RRID:Addgene_34960 Copy
Species: Homo sapiens
Genetic Insert: Ube1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:PET21d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21771579
Proper citation: RRID:Addgene_34965 Copy
Genetic Insert: PSmOrange2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5677; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22900938
Comments: https://www.ncbi.nlm.nih.gov/nuccore/JQ392581
Proper citation: RRID:Addgene_34964 Copy
Genetic Insert: LAP2-CFP
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pDisplay; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_34842 Copy
Genetic Insert: TCF binding sites
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pJIM20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18724937
Comments: Seven copies of the TCF binding site, AGATCAAAGG, were transferred from Super8XTOPflash (plasmid M50) (Veeman et al., 2003) into Fire lab vector L3135 to place them downstream of the pes-10 minimal promoter. The seven TCF sites and the pes-10 minimal promoter were amplified using forward primer AAGCTTGGTACCGAGCTCGG and reverse primer ATGCCTAGGCAATCAATGCCTGAAAGTTAAAAATTAC. The product was then cloned into mCherry plasmid (PJIM20) with let-858 3’ UTR (kind gift from Jon Audhya) using sites SpeI and AvrII.
There is a known sequence discrepancy between the depositor's and Addgene's sequence, which does not alter the function of this plasmid.
Proper citation: RRID:Addgene_34848 Copy
Genetic Insert: PSmOrange2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22900938
Comments: https://www.ncbi.nlm.nih.gov/nuccore/JQ392581
Proper citation: RRID:Addgene_34958 Copy
Vector Backbone Description: Backbone Marker:Turner and Weintraub (1994); Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Sea urchin, zebrafish, Xenopus ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124201
Proper citation: RRID:Addgene_34931 Copy
Species: Homo sapiens
Genetic Insert: p110β
Vector Backbone Description: Backbone Marker:Aoki et al., PNAS. 1998 Dec 8;95(25):14950-5; Backbone Size:2949; Vector Backbone:pBSFI; Vector Types:Adaptor plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432180
Proper citation: RRID:Addgene_34891 Copy
Vector Backbone Description: Backbone Marker:Turner and Weintraub (1994); Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Sea urchin, zebrafish, Xenopus ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124201
Proper citation: RRID:Addgene_34935 Copy
Genetic Insert: CAG-Floxed Synaptophysin-tdTomato
Vector Backbone Description: Backbone Size:13898; Vector Backbone:Rosa26 Targeting Vector; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22446880
Proper citation: RRID:Addgene_34881 Copy
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBluescriptII; Vector Types:Moss Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18803774
Comments: Scientists using this plasmid in a publication should acknowledge Drs. Tomoaki Nishiyama and Mitsuyasu Hasebe in the publication.
Proper citation: RRID:Addgene_34888 Copy
Genetic Insert: Lck-GCaMP5G
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3950; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23035093
Comments: Lck-GCaMP5G is a fusion between the Lck domain (https://www.ncbi.nlm.nih.gov/pubmed/21205365) and cytosolic GCaMP5G (http://www.addgene.org/31788). This construct targets GCaMP5G to the plasma membrane.
Proper citation: RRID:Addgene_34924 Copy
Vector Backbone Description: Vector Backbone:None; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:21868676
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which catalyzes the last step in serine biosynthesis, was deleted from Escherichia coli strain BL21. Markerless gene deletions were carried out using a λ-red and FLP recombinase-based gene knockout strategy.
Proper citation: RRID:Addgene_34929 Copy
Species: Mus musculus
Genetic Insert: LIM homeobox transcription factor 1-beta
Vector Backbone Description: Backbone Marker:Malin Parmar; Backbone Size:7041; Vector Backbone:pCCL-cppt-PGK-WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21646515
Proper citation: RRID:Addgene_34997 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpyCatcher
Vector Backbone Description: Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22366317
Proper citation: RRID:Addgene_35044 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ePDZb1–mCherry
Vector Backbone Description: Vector Backbone:YIPlac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Proper citation: RRID:Addgene_34981 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ePDZb–mCherry
Vector Backbone Description: Vector Backbone:YIPlac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Proper citation: RRID:Addgene_34980 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mid2(SS/TM)-GFP-LOVpep
Vector Backbone Description: Backbone Size:4263; Vector Backbone:YIPlac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388287
Comments: The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.
Proper citation: RRID:Addgene_34971 Copy
Species: Homo sapiens
Genetic Insert: FEN1DA
Vector Backbone Description: Backbone Marker:Stratgene; Backbone Size:7500; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20551483
Proper citation: RRID:Addgene_35028 Copy
Species: Homo sapiens
Genetic Insert: SRC
Vector Backbone Description: Backbone Size:8100; Vector Backbone:MSCV-IP N-HAonly; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22232672
Proper citation: RRID:Addgene_35017 Copy
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