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Vector Backbone Description: Backbone Marker:Miguel Valvano and Silvia Cardona (Addgene plasmid # 113634); Backbone Size:7518; Vector Backbone:pSCrhaB2; Vector Types:Bacterial Expression; Bacterial Resistance:Trimethoprim
Defining Citation: PMID:34190606
Comments: This plasmid is derived from pSCrhaB2 (Addgene plasmid #113634).
Reference for pSCrhaB2:
Cardona ST, Valvano MA. Plasmid. 2005 Nov;54(3):219-28. doi: 10.1016/j.plasmid.2005.03.004
Proper citation: RRID:Addgene_164226 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:8370; Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164229 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:8370; Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164228 Copy
Genetic Insert: sg p53
Vector Backbone Description: Backbone Size:9265; Vector Backbone:pU6 Cas9-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476576
Proper citation: RRID:Addgene_164256 Copy
Species: Homo sapiens
Genetic Insert: MYC
Vector Backbone Description: Backbone Size:9354; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_164145 Copy
Vector Backbone Description: Backbone Size:5352; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33271061
Comments: Reference for vector backbone.
Guzman, L. M., Belin, D., Carson, M. J., & Beckwith, J. (1995). Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. Journal of Bacteriology, 177(14), 4121–4130.
Proper citation: RRID:Addgene_164263 Copy
Species: E. coli
Genetic Insert: miniTn6900 element
Vector Backbone Description: Backbone Size:13150; Vector Backbone:pMS26; Vector Types:Bacterial delivery vector for Tn7 att site; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33271061
Comments: This vector is based on pMS26 that is also available from Addgene and is described in the following reference.
Sibley, M. H., & Raleigh, E. A. (2012). A versatile element for gene addition in bacterial chromosomes. Nucleic Acids Research, 40(3), e19–e19. http://doi.org/10.1093/nar/gkr1085
Proper citation: RRID:Addgene_164260 Copy
Species: Mus musculus
Genetic Insert: Plexin A2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:In Situ Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11688557
Comments: This plasmid is an improved form of the mPlexin A2 in situ template plasmid originally described in Brown CB, Feiner L, Lu MM, Li J, Ma X, Webber AL, Jia L, Raper JA, Epstein JA. PlexinA2 and semaphorin signaling during cardiac neural crest development.
Development. 2001 128(16):3071-80. Anti-sense in situ probe is generated by linearizing with BamHI and transcribing with T3 RNA polymerase.
Proper citation: RRID:Addgene_16423 Copy
Species: Homo sapiens
Genetic Insert: ACE2
Vector Backbone Description: Backbone Marker:Addgene plasmid 67974; Vector Backbone:pKLV2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33103998
Proper citation: RRID:Addgene_164219 Copy
Species: Homo sapiens
Genetic Insert: opto-RhoA
Vector Backbone Description: Backbone Size:5372; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34288599
Proper citation: RRID:Addgene_164472 Copy
Species: Homo sapiens
Genetic Insert: mGreenLantern
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33208539
Comments: Cre-inducible expression (double floxed inverted ORF)
Proper citation: RRID:Addgene_164468 Copy
Vector Backbone Description: Backbone Size:6637; Vector Backbone:pSL21-mCherry; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33636129
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.20.087379v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_164410 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:Backbone derived from pEF-GFP (Addgene #11154); Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33317513
Proper citation: RRID:Addgene_164411 Copy
Genetic Insert: ABEmax
Vector Backbone Description: Vector Backbone:Derived from pCMV-ABEmax (Addgene #112095); Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33317513
Proper citation: RRID:Addgene_164415 Copy
Species: Gallus gallus
Genetic Insert: TVA
Vector Backbone Description: Backbone Size:4500; Vector Backbone:biCS2 (bicistronic pCS2); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34099488
Proper citation: RRID:Addgene_164486 Copy
Species: Firefly
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33412089
Proper citation: RRID:Addgene_164432 Copy
Genetic Insert: p53-binding site (mutant)
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8242752
Comments: MG15-Luc was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 2.6 kb SacI luciferase cassette without promoter elements, were then cloned downstream.
MG15 contains 15 copies of a mutated p53-binding sequence. Addgene has confirmed the presence of 8 of these copies.
Direct repeats of the oligonucleotide MG (5'-CCTTAATGGACTTTAATGG-
3') were used for the
p53 nonbinding control sequences.
Proper citation: RRID:Addgene_16443 Copy
Species: Homo sapiens
Genetic Insert: MDM2
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1614537
Comments: Full-length human MDM2 cDNA.
Proper citation: RRID:Addgene_16441 Copy
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2144057
Comments: The vector pCMV-Neo-Bam was engineered to contain two independent transcription units. The first unit is comprised of a CMV promoter/enhancer upstream of a multiple cloning site, and splice and polyA sites to ensure appropriate processing. The second transcription unit includes a herpes simplex virus (HSV) thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.
Proper citation: RRID:Addgene_16440 Copy
Genetic Insert: SARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33412089
Proper citation: RRID:Addgene_164437 Copy
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