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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pB194 Resource Report Resource Website |
RRID:Addgene_61154 | TFIIH complex serine/threonine-protein kinase subunit KIN28 | Saccharomyces cerevisiae | Ampicillin | PMID:11805111 | Backbone Marker:Herren, B., and Pech, M. (1993) J. Recept. Res. 13, 725–738; Vector Backbone:pRS425Gal1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:39 | 0 | ||
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pMTB-Multibow-hY Resource Report Resource Website |
RRID:Addgene_60995 | citrine | Synthetic | Ampicillin | PMID:26010570 | Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region. | Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 0 | |
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pMTB-Multibow-hG Resource Report Resource Website |
RRID:Addgene_60994 | EGFP | Synthetic | Ampicillin | PMID:26010570 | Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region. | Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 0 | |
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pMTB-Multibow-hO Resource Report Resource Website |
RRID:Addgene_60996 | mKO | Synthetic | Ampicillin | PMID:26010570 | Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region. | Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 0 | |
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Prp2-pRSETA Resource Report Resource Website |
RRID:Addgene_61042 | Prp2 | Saccharomyces cerevisiae | Ampicillin | PMID:24240612 | Backbone Marker:Invitrogen Life Technologies; Backbone Size:2900; Vector Backbone:pRSETA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 0 | ||
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Cwc25-pRSETA Resource Report Resource Website |
RRID:Addgene_61043 | Cwc25 | Saccharomyces cerevisiae | Ampicillin | PMID:24240612 | Backbone Marker:Invitrogen Life Technologies; Backbone Size:2900; Vector Backbone:pRSETA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:38 | 0 | ||
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pBabe-Hygro-FLAG-hDIXDC1 WT Resource Report Resource Website |
RRID:Addgene_61213 | human DIXDC1 | Homo sapiens | Ampicillin | PMID:25042806 | Backbone Size:5200; Vector Backbone:pBabe-Hygro-DEST; Vector Types:Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:39 | 0 | ||
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pLenti-CMV/TO-Puro-FLAG-hDIXDC1 WT Resource Report Resource Website |
RRID:Addgene_61218 | human DIXDC1 | Homo sapiens | Ampicillin | PMID:25042806 | Backbone Size:8410; Vector Backbone:pLenti-CMV/TO-PURO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:39 | 0 | ||
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pmIL-6 mut AP-1+C/EBP Resource Report Resource Website |
RRID:Addgene_61292 | IL-6 promoter | Mus musculus | Ampicillin | PMID:12626566 | Contains point mutations in both AP-1 and C/EBP binding sites. See Table 1 of associated publication for more details. Full sequence and map is available for wild-type vector pmIL-6 FL (Plasmid #61290) | Backbone Marker:Promega; Vector Backbone:pGL2 basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | Mutated both AP-1 binding site from TGAGTCT to TGCAGCT and C/EBP binding site from ACATTGTGCAATCT to ACAGATATCAATCT | 2026-08-15 01:17:40 | 0 |
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pQCXIB-FLAG-hDIXDC1-L-S592A Resource Report Resource Website |
RRID:Addgene_61225 | human DIXDC1 | Homo sapiens | Ampicillin | PMID:25042806 | Backbone Marker:Addgene #17400; Backbone Size:8614; Vector Backbone:pQCXIB; Vector Types:Retroviral; Bacterial Resistance:Ampicillin | Changed Serine 592 to Alanine | 2026-08-15 01:17:40 | 0 | |
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pcDNA3-FLAG-hDIXDC1 S592A Resource Report Resource Website |
RRID:Addgene_61223 | human DIXDC1 | Homo sapiens | Ampicillin | PMID:25042806 | Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Changed Serine 592 to Alanine | 2026-08-15 01:17:39 | 0 | |
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pLentiX2-PURO-shDIXDC1-Ms-58 Resource Report Resource Website |
RRID:Addgene_61233 | Dixdc1 | Mus musculus | Ampicillin | PMID:25042806 | Backbone Marker:Eric Campeau; Vector Backbone:pLentiX2-PURO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:40 | 0 | ||
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pLentiX2-PURO-shDIXDC1-Ms-57 Resource Report Resource Website |
RRID:Addgene_61232 | Dixdc1 | Mus musculus | Ampicillin | PMID:25042806 | Backbone Marker:Eric Campeau; Vector Backbone:pLentiX2-PURO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:40 | 0 | ||
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AAV-hSyn-REX-GECO1 Resource Report Resource Website |
RRID:Addgene_61248 | REX-GECO1 | Synthetic | Ampicillin | PMID:25358432 | The construct is numbered based on GCaMP. There are 2 mismatches between Addgene's sequence and the provided reference sequence. These should not affect plasmid function. | Backbone Size:4560; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | Substitutions relative to R-GECO1: P60R, V61W, R66W, E77V, K80E, K97R, S142P, D147V, E148G, P220L, N257I, A302P, M339L, T382S | 2026-08-15 01:17:40 | 0 |
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CMV-REX-GECO0.9 Resource Report Resource Website 1+ mentions |
RRID:Addgene_61247 | REX-GECO0.9 | Synthetic | Ampicillin | PMID:25358432 | The construct is numbered based on GCaMP. | Backbone Size:3200; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Substitutions relative to R-GECO1: P60R, V61W, R66W, E77V, K80E, K97R, E138V, S142P, D147V, P220L, N257I, N267D, A302P, M339L, T382S | 2026-08-15 01:17:40 | 1 |
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AAV-hSyn-REX-GECO0.9 Resource Report Resource Website |
RRID:Addgene_61249 | REX-GECO0.9 | Synthetic | Ampicillin | PMID:25358432 | The construct is numbered based on GCaMP. There are 2 mismatches between Addgene's sequence and the provided reference sequence. These should not affect plasmid function. | Backbone Size:4560; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | Substitutions relative to R-GECO1: P60R, V61W, R66W, E77V, K80E, K97R, E138V, S142P, D147V, P220L, N257I, N267D, A302P, M339L, T382S | 2026-08-15 01:17:40 | 0 |
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CMV-ER-LAR-GECO1 Resource Report Resource Website 10+ mentions |
RRID:Addgene_61244 | LAR-GECO1 | Synthetic | Ampicillin | PMID:25164254 | The construct is numbered based on GCaMP. | Backbone Size:3200; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Substitutions relative to R-GECO1: V51W/I113V/N356S/D363Y/D381Y/F395A/V411A/L415I | 2026-08-15 01:17:40 | 17 |
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pLentiX2-PURO-shLKB1-Hu Resource Report Resource Website 1+ mentions |
RRID:Addgene_61242 | LKB1 | Homo sapiens | Ampicillin | PMID:25042806 | Backbone Marker:Eric Campeau; Vector Backbone:pLentiX2-PURO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:40 | 2 | ||
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pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5 Resource Report Resource Website |
RRID:Addgene_61257 | 2xTY1-V5 | Synthetic | Ampicillin | PMID:25324299 | Backbone Marker:Hugo J Bellen; Backbone Size:3300; Vector Backbone:pBS-KS-attB1-2-PT-SA-SD; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:40 | 0 | ||
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pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5 Resource Report Resource Website |
RRID:Addgene_61256 | 2xTY1-V5 | Synthetic | Ampicillin | PMID:25324299 | Backbone Marker:Hugo J Bellen; Backbone Size:3300; Vector Backbone:pBS-KS-attB1-2-PT-SA-SD; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:40 | 0 |
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