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Species: Saccharomyces cerevisiae
Genetic Insert: Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS607c
Vector Backbone Description: Vector Backbone:p426; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27899650
Proper citation: RRID:Addgene_87388 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUS1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87557 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87592 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP28
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87595 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP43
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87596 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP9
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87593 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP11
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87594 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CLF1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87548 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LifeAct-Venus
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26748717
Proper citation: RRID:Addgene_87613 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LifeAct-FRB-Venus173-N
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26748717
Proper citation: RRID:Addgene_87616 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dronpa-FHA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28288122
Proper citation: RRID:Addgene_87711 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Lyn-FLINC-EKAR1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28288122
Proper citation: RRID:Addgene_87707 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLINC-AKAR1-CAAX
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28288122
Proper citation: RRID:Addgene_87705 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGAL1-mKate2
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pNH603; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Proper citation: RRID:Addgene_87947 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NUF2-sGFP
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7568202
Comments: 2 micron plasmid.
Proper citation: RRID:Addgene_8850 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sec63-sGFP
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10931860
Comments: Constructed by amplifying SEC63 and ~400 bp of its 5' promoter from pPS599 (R. Schekman, UC Berkeley). Product cloned between XbaI and XhoI sites of CEN/URA3 plasmid, pJK50 (Kahana, MBC 1998).
Proper citation: RRID:Addgene_8854 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NIP100
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9658168
Comments: Integrating Nip100, 3xHA, NUF2 3'UTR plasmid. Rescues nip100delta; cin8delta. Cut with ClaI before transforming. Note that XhoI fusion site was destroyed because SalI was used to clone Nip100 (which has an internal XhoI site).
Proper citation: RRID:Addgene_8852 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Backbone Size:5181; Vector Backbone:YEp352; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: GAL1 promoter (EcoRI/XbaI fragment from pPS231), 2 micron plasmid. See author's map.
Proper citation: RRID:Addgene_8851 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HRB1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9499403
Comments: GST-HRB1 was excised from pPS1320 and ligated into pGEX-4T-1. See author's map.
Proper citation: RRID:Addgene_8913 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YRB2
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pPS293; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9395535
Comments: GAL1 promoter, 2 micron plasmid.
Proper citation: RRID:Addgene_8910 Copy
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