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Species: Saccharomyces cerevisiae
Genetic Insert: NPL3 F160L
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pPS811; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8675010
Comments: GAL1 promoter, 2 micron plasmid. 278 bp BglII fragment from pPS877 in BglII linear pPS811.
Proper citation: RRID:Addgene_8906 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NPL3
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pPS808; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8675010
Comments: GAL1 promoter, 2 micron plasmid. 1.5kb BamHI fragment from pPS425. See author's map.
Proper citation: RRID:Addgene_8904 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Clb2-deltaNLS-GFP
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pPS1304; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11171327
Comments: Similar to pPS2189, but with deletion of NLS. Created by PCR amplifying the fragments of CLB2 ORF upstream (0.5kb) and downstream (0.9kb) of the bipartite NLS and joining them using KpnI ends. The KpnI site introduces a glycine and a threonine in place of the bipartite NLS. 2 micron plasmid.
Proper citation: RRID:Addgene_8923 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HRB1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pPS1332; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9499403
Comments: 1.3-kb HRB1-myc PCR product in pPS1332, Gal1 promoter, 2 micron plasmid. See author's map.
Proper citation: RRID:Addgene_8915 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HRB1
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9499403
Comments: See author's map.
Proper citation: RRID:Addgene_8914 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pZ-YFP
Vector Backbone Description: Backbone Size:7294; Vector Backbone:pNH604; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Proper citation: RRID:Addgene_89194 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pZ-YFP
Vector Backbone Description: Backbone Size:6559; Vector Backbone:pNH604; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Proper citation: RRID:Addgene_89193 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGAL1-mCherry
Vector Backbone Description: Backbone Size:6559; Vector Backbone:pNH604; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Comments: The mCherry insert has a G121D mutation that the depositor has confirmed does not affect plasmid function.
Proper citation: RRID:Addgene_89192 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGAL1-YFP
Vector Backbone Description: Backbone Size:7366; Vector Backbone:pNH605; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Proper citation: RRID:Addgene_89191 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pZ-mCherry
Vector Backbone Description: Backbone Size:6559; Vector Backbone:pNH604; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Proper citation: RRID:Addgene_89196 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RST1
Vector Backbone Description: Vector Backbone:604 TRP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Comments: The Dig1 insert contains a S110G mutation which the depositor has confirmed does not affect plasmid function.
Proper citation: RRID:Addgene_89199 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MSG 5
Vector Backbone Description: Vector Backbone:604 TRP; Vector Types:yeast integration; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Comments: Linearize by digesting with Pme1. The MSG5 insert contains a K130R mutation that the depositor has confirmed does not affect plasmid function.
Proper citation: RRID:Addgene_89200 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GSP1
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pPS293; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7816822
Comments: Gal-driven GSP1, myc tagged, 2 micron origin. Compare with pPS832.
Proper citation: RRID:Addgene_8932 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vacuolar protein sorting-associated protein 4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5700; Vector Backbone:pET151; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28379137
Proper citation: RRID:Addgene_89287 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP16
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26968627
Proper citation: RRID:Addgene_89972 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP16
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26968627
Proper citation: RRID:Addgene_89971 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP16
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26968627
Proper citation: RRID:Addgene_89973 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26968627
Proper citation: RRID:Addgene_89968 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26968627
Proper citation: RRID:Addgene_89967 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BRR2
Vector Backbone Description: Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2017
Proper citation: RRID:Addgene_90257 Copy
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