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Species: Homo sapiens
Genetic Insert: deleted in breast cancer 1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA 3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20160719
Proper citation: RRID:Addgene_35096 Copy
Species: Mus musculus
Genetic Insert: Paired box protein Pax-2
Vector Backbone Description: Backbone Marker:Malin Parmar; Backbone Size:7041; Vector Backbone:pCCL-cppt-PGK-WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21646515
Proper citation: RRID:Addgene_35002 Copy
Species: Homo sapiens
Genetic Insert: TIMP1
Vector Backbone Description: Backbone Size:8100; Vector Backbone:MSCV-IP N-HAonly; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22232672
Proper citation: RRID:Addgene_35009 Copy
Genetic Insert: Split luciferase for GZF1/3 SSA assay
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-control; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20680825
Comments: Originally described in Szczepek, M., Brondani, V., Büchel, J., Serrano, L., Segal, D.J. and Cathomen, T. (2007) Structure-based redesign of the dimerization interface reduces the toxicity of zinc finger nucleases. Nat. Biotechnol., 25:786-793.
Proper citation: RRID:Addgene_35091 Copy
Genetic Insert: SpyTagMBP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22366317
Proper citation: RRID:Addgene_35050 Copy
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11976; Vector Backbone:pBID-UASC-GRM; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of myc tagRFP (tRFP) fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). myc-tRFP is fused to the Cterminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
Proper citation: RRID:Addgene_35203 Copy
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11145; Vector Backbone:pBID-UASC-G; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31transgenic Drosophila for GAL4 driven expression. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter(DSCP). Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_35202 Copy
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11240; Vector Backbone:pBID-UASC-FG; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of Flag epitope fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). 3 copies of the Flag epitope is fused to the N-terminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_35201 Copy
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11858; Vector Backbone:pBID-UASC-VG; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of mVenus fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). mVenus is fused to the N-terminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_35206 Copy
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11858; Vector Backbone:pBID-UASC-GV; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of mVenus fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). mVenus is fused to the C-terminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. chloramphenicol resistant for amplification.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_35204 Copy
Species: Homo sapiens
Genetic Insert: Myc promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8972190
Comments: This plasmid is a c-myc promoter-luciferase reporter construct designed to examine the regulation of transcription in response to conditional and constitutive Myc activation and functions as described in the associated publication.
Addgene's sequencing results identified a V13C mutation in exon 1 of c-myc when compared to GenBank reference sequence AAA59886.1
Proper citation: RRID:Addgene_35155 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
Vector Backbone Description: Vector Backbone:pBbA5c; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21952217
Comments: Please note that the R364 in Mevalonate Kinase (MK) may be susceptible to mutations. Although the original plasmid encoded R364C, Addgene's stock contains R364S. This mutation is not expected to impact overall function, however the exact effects are unknown.
Proper citation: RRID:Addgene_35152 Copy
Species: Homo sapiens
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22072788
Proper citation: RRID:Addgene_35140 Copy
Species: Human papillomavirus type 45
Genetic Insert: HPV45 E7
Vector Backbone Description: Vector Backbone:MSCV-P C-Flag-HA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22232672
Proper citation: RRID:Addgene_35135 Copy
Species: Homo sapiens
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22072788
Proper citation: RRID:Addgene_35139 Copy
Species: Homo sapiens
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22072788
Proper citation: RRID:Addgene_35138 Copy
Species: Streptomyces noursei
Genetic Insert: nat1
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10514571
Proper citation: RRID:Addgene_35126 Copy
Species: Streptomyces viridochromogenes
Genetic Insert: patMX3
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10514571
Proper citation: RRID:Addgene_35124 Copy
Species: Candida albicans
Genetic Insert: URA3
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10514571
Proper citation: RRID:Addgene_35129 Copy
Species: Klebsiella pneumoniae
Genetic Insert: hphMX4
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10514571
Proper citation: RRID:Addgene_35122 Copy
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