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Species: BK polyomavirus
Genetic Insert: BKV ER
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: There is a splice site in the DNA to make a truncated Lg T (348bp), as well as a full length small T, both tagged at N-terminal end.
The small and Lg T seqences overlap for the first 242 bp. (genome entry, BKV, strain UT: https://www.ncbi.nlm.nih.gov/nucleotide/83415612?report=genbank&log$=nuclalign&blast_rank=4&RID=53X3T5WA016 )
Both proteins are seen when this construct is expressed in cells.
Proper citation: RRID:Addgene_37864 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Though there are some discrepancies between the depositor sequence and Addgene's quality control sequence, they are not known to affect plasmid function.
Proper citation: RRID:Addgene_37855 Copy
Species: Simian Virus 40
Genetic Insert: SV40 ST
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [STFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37858 Copy
Species: codon-modified firefly (Photinus pyralis)
Genetic Insert: 3 copies of ERRE upstream of luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3-Promoter; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24684682
Proper citation: RRID:Addgene_37851 Copy
Species: Rattus norvegicus
Genetic Insert: CL1
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:9761; Vector Backbone:pAcGP67A; Vector Types:Baculovirus Transfer Vectors; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22333914
Proper citation: RRID:Addgene_37847 Copy
Species: Homo sapiens
Genetic Insert: BAI3
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:9761; Vector Backbone:pAcGP67A; Vector Types:Baculovirus Transfer Vectors; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22333914
Proper citation: RRID:Addgene_37848 Copy
Species: Mus musculus
Genetic Insert: Estrogen-related receptor gamma
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24684682
Proper citation: RRID:Addgene_37849 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pnhx-2cpl-1::YFP
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37834 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pnhx-2cpl-1W32A;Y35A::YFP
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37835 Copy
Genetic Insert: Pnhx-2mCherry
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37832 Copy
Species: Caenorhabditis elegans
Genetic Insert: F13D12.6(G166R)
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37837 Copy
Genetic Insert: Pnhx-2DsRed::KDEL
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37838 Copy
Species: Caenorhabditis elegans
Genetic Insert: Ub-V
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37839 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BFRF0.5
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein
Though there are some discrepancies between the depositor sequence and Addgene's quality control sequence, they are not known to affect plasmid function.
Proper citation: RRID:Addgene_37932 Copy
Species: bacteria
Genetic Insert: Promotorless gfp reporter gene
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11059491
Comments: Please refer to the attached table for a complete list of restriction sites in the MCS.
Proper citation: RRID:Addgene_37812 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BGLF3
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37934 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BGLF3.5
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37935 Copy
Species: H. sodomense
Genetic Insert: Arch
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:4722; Vector Backbone:AAV with CAG promoter; Vector Types:Mammalian Expression, AAV, Adeno-associated virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20054397
Comments: PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.
Proper citation: RRID:Addgene_37810 Copy
Genetic Insert: BFLF2
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37931 Copy
Species: HPV
Genetic Insert: HPV 5 E6
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-C FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37888 Copy
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