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Vector Backbone Description: Backbone Size:6500; Vector Backbone:pSR47S; Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB; Bacterial Resistance:Chloramphenicol
Comments: Plasmid pSR47S Cm was constructed by removing the KmR region from pSR47S by PCR amplification of the residual plasmid, then cloning in the CmR gene. The CmR gene was amplified from a pMMB207 plasmid by PCR, with restriction sites that allowed cloning into pSR47S that lacked the KmR region.
Proper citation: RRID:Addgene_41892 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: PpsbAI::luxCDE, PpsbAI::luxAB
Vector Backbone Description: Vector Backbone:pAM1582; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17417005
Comments: NSII.1 vector
Lux Genes: luxAB & luxCDE
synthesizes luciferase and substrate in S. elongatus
SmaI--PvuII fragment containing PpsbAI::luxCDE was cloned into SmaI site of pAM1582. Orientation of PpsbAI::luxCDE is same as that of PpsbAI::luxAB. NS2.1, PpsbAI::luxCDE, PpsbAI::luxAB plasmid.
Proper citation: RRID:Addgene_40245 Copy
Species: Arabidopsis thaliana
Genetic Insert: 4-coumaroyl CoA ligase 1
Vector Backbone Description: Backbone Size:4449; Vector Backbone:pAC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:16551366
Comments: Initial gene cloning described in the following:
"Exploring Recombinant Flavonoid Biosynthesis in Metabolically Engineered Escherichia coli" (2004)Kevin T. Watts, Pyung Cheon Lee Dr., Claudia Schmidt-Dannert Prof. ChemBioChem. DOI: 10.1002/cbic.200300783
Proper citation: RRID:Addgene_35947 Copy
Genetic Insert: HEH-3
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36282 Copy
Genetic Insert: HE-6
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36279 Copy
Genetic Insert: HE-5
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36278 Copy
Genetic Insert: EH-4
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36273 Copy
Genetic Insert: EH-3
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36272 Copy
Genetic Insert: EH-2
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36271 Copy
Genetic Insert: E. coli KdtA
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36268 Copy
Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36267 Copy
Species: Bergeyella zoohelcum
Genetic Insert: BzCas13b-gRNA-3
Vector Backbone Description: Backbone Size:4215; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34694607
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164860 Copy
Species: Leptotrichia buccalis
Genetic Insert: AcrVIA7
Vector Backbone Description: Backbone Size:4010; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34694607
Comments: The anti-CRISPR protein AcrVIA7 was codon-optimized for E. coli for optimal expression.
Proper citation: RRID:Addgene_164864 Copy
Species: Bergeyella zoohelcum
Genetic Insert: BzCas13b-gRNA-1
Vector Backbone Description: Backbone Size:4215; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34694607
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164858 Copy
Species: Synthetic
Genetic Insert: CT81 gene circuit
Vector Backbone Description: Backbone Size:9311; Vector Backbone:pMMB206; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33558556
Comments: pMMB206 backbone is very low copy, <10 copies.
Proper citation: RRID:Addgene_165411 Copy
Species: Viral
Genetic Insert: _C31 attB
Vector Backbone Description: Backbone Size:2171; Vector Backbone:pColE1_Omega1; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: attB docking site
Proper citation: RRID:Addgene_165843 Copy
Vector Backbone Description: Backbone Size:6204; Vector Backbone:P[acman]; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: Omega1
Proper citation: RRID:Addgene_165860 Copy
Vector Backbone Description: Backbone Size:6204; Vector Backbone:P[acman]; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: Omega2
Proper citation: RRID:Addgene_165861 Copy
Species: Synthetic
Genetic Insert: QFAD
Vector Backbone Description: Backbone Size:2343; Vector Backbone:pUPD3; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: CDS-TL5
Proper citation: RRID:Addgene_165824 Copy
Species: Drosophila melanogaster
Genetic Insert: 3xP3
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: Enhancer
Proper citation: RRID:Addgene_165779 Copy
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