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Species: Synthetic
Genetic Insert: hygromycin phosphotranferase (hptII) (pCAMBIA)
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54463 Copy
Species: Synthetic
Genetic Insert: superfolder GFP 1-10 domain
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Comments: Please note this plasmid contains super-folder GFP and shows noticeable background fluorescence.
Proper citation: RRID:Addgene_54461 Copy
Species: Discosoma sp.
Genetic Insert: E2-Crimson
Vector Backbone Description: Vector Backbone:pUCP30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26937640
Comments: There is an ER retention signal at the C-terminus of the E2-Crimson ORF. This sequence is based on the template used to generate this vector. It is not predicted to impact expression or function in bacteria.
Proper citation: RRID:Addgene_78478 Copy
Vector Backbone Description: Backbone Marker:Jurgen Quandt; Backbone Size:4700; Vector Backbone:pJQ200; Vector Types:Suicide vector for gene replacement; Bacterial Resistance:Gentamicin
Defining Citation: PMID:8486283
Comments: Allows different restriction cloning strategies to be used than pJQ200SK. Since full length lacZ alpha is present, blue white selection works faster than for pJQ200SK
Proper citation: RRID:Addgene_78501 Copy
Vector Backbone Description: Backbone Marker:Jurgen Quandt; Backbone Size:4700; Vector Backbone:pJQ200; Vector Types:Suicide vector for gene replacement; Bacterial Resistance:Gentamicin
Defining Citation: PMID:8486283
Comments: Allows different restriction cloning strategies to be used than pJQ200SK. Since full length lacZ alpha is present, blue white selection works faster than for pJQ200SK
Proper citation: RRID:Addgene_78500 Copy
Vector Backbone Description: Backbone Marker:Jurgen Quandt; Backbone Size:4700; Vector Backbone:pJQ200; Vector Types:Suicide vector for gene replacement; Bacterial Resistance:Gentamicin
Defining Citation: PMID:8486283
Comments: The new synthetic polylinker (SmaI site flanked by NotI) allows access to restriction sites within a cloned fragment to allow insertion of cassettes etc. Blue/White selection works.
Proper citation: RRID:Addgene_78502 Copy
Vector Backbone Description: Backbone Size:4922; Vector Backbone:pJQ200; Vector Types:Suicide plasmid; Bacterial Resistance:Gentamicin
Defining Citation: PMID:8486283
Comments: This is a suicide vector for gene replacement. It is identical to pJQ200SK except that the multiple cloning site is from pBluescriptKS instead of from pBluescriptSK
Proper citation: RRID:Addgene_78498 Copy
Species: Chlorocebus pygerythrus pygerythrus
Genetic Insert: OSF-TRIM5alpha(African green monkey pygerythrus)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27253068
Comments: Note that the TRIM5 insert in this plasmid contains a V434A polymorphism compared to GenBank ID AAW72440.1. Please see the full plasmid sequence for the most accurate information.
Proper citation: RRID:Addgene_79031 Copy
Species: Homo sapiens
Genetic Insert: OSF-TRIM5alpha(human)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27253068
Proper citation: RRID:Addgene_79034 Copy
Species: Chlorocebus pygerythrus pygerythrus
Genetic Insert: OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27253068
Proper citation: RRID:Addgene_79030 Copy
Species: Aotus trivirgatus
Genetic Insert: OSF-TRIM5Cyp (owl monkey)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27253068
Proper citation: RRID:Addgene_79035 Copy
Species: Macaca mulatta
Genetic Insert: rhesus TRIM5alha-PFOS
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27253068
Comments: The depositor-provided reference sequence shows M221T and P307T compared to DQ842021.1
Proper citation: RRID:Addgene_79038 Copy
Species: Homo sapiens
Genetic Insert: OSF-TRIM5alpha(human R332P)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27253068
Proper citation: RRID:Addgene_79037 Copy
Genetic Insert: NA
Vector Backbone Description: Vector Backbone:NA; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26829286
Comments: If LacI is added to the cell (e.g., in a plasmid), the expression of RFP will be somewhat repressed (unless if IPTG is added), as there is a lacO between the promoter and the gene's ATG. Attempts to remove this lacO were unsuccessful - we suspect the higher RFP expression might lead to cell death (in the cloning strain, E. coli DH5alpha).
Please note this strain has a higher %GC than E. coli, so PCRing (and sequencing) may be difficult.
Proper citation: RRID:Addgene_79642 Copy
Genetic Insert: oriR6K
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:15908923
Comments: Genbank Accession #AY884833
Proper citation: RRID:Addgene_64966 Copy
Genetic Insert: FRT-GmR-FRT
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pFRT2; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:15908923
Comments: GenBank Accession #AY597270
Compared to the Genbank reference sequence AY597270, Addgene's sequencing results found a R98K mutation in the gentamycin cassette. The effect of this difference is unknown.
Proper citation: RRID:Addgene_64949 Copy
Vector Backbone Description: Backbone Marker:Beckwith Lab; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:19151135
Proper citation: RRID:Addgene_65098 Copy
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:17406227
Comments: GenBank Accession # DQ296496
Compared to the Genbank reference sequence DQ296496, Addgene's sequencing results found T920I, D999E and F1000L mutations in lacZ. The effect of these differences is unknown. Note that the amino acid numbering is relative to the lacZ encoded by this plasmid.
Proper citation: RRID:Addgene_65028 Copy
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18R6K-mini-Tn7T; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:17406227
Comments: GenBank Accession # AY599228
Proper citation: RRID:Addgene_65022 Copy
Genetic Insert: ecfp
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:17406227
Comments: GenBank Accession # DQ493878
Proper citation: RRID:Addgene_65030 Copy
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