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Vector Backbone Description: Backbone Marker:Addgene plasmid #1764; Backbone Size:4517; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22371484
Proper citation: RRID:Addgene_36999 Copy
Species: Homo sapiens
Genetic Insert: Dsg2
Vector Backbone Description: Backbone Marker:K. Green lab; Vector Backbone:pmCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22184201
Proper citation: RRID:Addgene_36991 Copy
Species: Bovine Papillomavirus
Genetic Insert: BPV1 E6
Vector Backbone Description: Vector Backbone:N-Tap-Flag-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22547818
Proper citation: RRID:Addgene_37047 Copy
Species: Homo sapiens
Genetic Insert: Smad4
Vector Backbone Description: Backbone Marker:Weinberg Lab; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21245094
Comments: Insert was blunt-ended and ligated into SnaBI-digested pBabe-puro vector
Proper citation: RRID:Addgene_37041 Copy
Species: Mus musculus
Genetic Insert: slug
Vector Backbone Description: Backbone Marker:Dr. Tal Kafri; Backbone Size:0; Vector Backbone:pTK380 (tetracycline inducible); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: Slug cDNA was PCR amplified from an IMAGE clone (Open Biosystems) and cloned into BamHI-XbaI sites of pTK380.
Proper citation: RRID:Addgene_36986 Copy
Species: Homo sapiens
Genetic Insert: Dsg2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987342
Comments: The construct encoding human Dsg2 cDNA in pBluescript was a gift from Drs. W. Franke and S. Schafer, German Cancer Research Center, Heidelberg, Germany.
Proper citation: RRID:Addgene_36989 Copy
Species: Mus musculus
Genetic Insert: Hes1
Vector Backbone Description: Backbone Marker:Trono lab (Addgene plasmid 12257); Backbone Size:10510; Vector Backbone:pWPXL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: The mouse Hes1 cDNA was cloned between MluI and SpeI sites (blunted) in pWPXL. This resulted in replacement of the EGFP in the vector backbone with mouse Hes1.
Proper citation: RRID:Addgene_36983 Copy
Species: Homo sapiens
Genetic Insert: beta-catenin deltaN90
Vector Backbone Description: Backbone Marker:I. Ben-Porath; Backbone Size:0; Vector Backbone:pLV-IRES-neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: The pLV-neo vector was modified from the attached vector by replacing dsRed with Neomycin resistant cassette and removing tTR-kRAB.
Addgene QC sequence analysis identified a S374G discrepancy, relative to reference sequence NP_001091679.1.
Proper citation: RRID:Addgene_36985 Copy
Species: Mus musculus
Genetic Insert: c-Myc
Vector Backbone Description: Backbone Marker:Trono lab (Addgene plasmid 12257); Backbone Size:10510; Vector Backbone:pWPXL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: The mouse c-myc cDNA was cloned between MluI and SpeI sites (blunted) in pWPXL. This resulted in replacement of the EGFP in the original vector with mouse c-myc.
Proper citation: RRID:Addgene_36980 Copy
Species: Mus musculus
Genetic Insert: Snail
Vector Backbone Description: Backbone Marker:Dr. Tal Kafri; Backbone Size:0; Vector Backbone:pTK380 (tetracycline inducible); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: Snail cDNA was PCR amplified from IMAGE clone and cloned into BamHI-XbaI sites of pTK380.
Proper citation: RRID:Addgene_36976 Copy
Species: Homo sapiens
Genetic Insert: Sox10
Vector Backbone Description: Backbone Marker:Kong-Jie Kah; Backbone Size:0; Vector Backbone:FUW-LPT2 (tetracycline-inducible); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: Human Sox10 cDNA, obtained from Harvard DNA Resource Core, was cloned between EcoRI and BamHI of FUW-LPT2.
Proper citation: RRID:Addgene_36978 Copy
Species: Homo sapiens
Genetic Insert: NRF2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22215675
Proper citation: RRID:Addgene_36971 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid #36953; Backbone Size:7052; Vector Backbone:pCMMP-MCS-IRES-eGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22318153
Comments: Sugden lab plasmid p3313
Addgene's sequencing results differ from the full plasmid sequence provided by the depositing by a single nucleotide insertion between bp# 2412&2413 and a four nucleotide deletion of bp #2493-2496. These differences are in IRES and the depositing laboratory verified the IRES is functional.
Proper citation: RRID:Addgene_36972 Copy
Species: Homo sapiens
Genetic Insert: MOB1B
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCl-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18362890
Proper citation: RRID:Addgene_37024 Copy
Species: Homo sapiens
Genetic Insert: RASSF6b
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCl-neo (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19797269
Proper citation: RRID:Addgene_37021 Copy
Species: Homo sapiens
Genetic Insert: rassf1A
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCl-neo (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19797269
Comments: pCIneoFH vector has the sequence NheI-FLAG-His6-EcoRI-MluI-XbaI-SalI-SmaI-NotI.
A digest with NheI and SalI will excise the insert with tags.
Proper citation: RRID:Addgene_37016 Copy
Species: Mus musculus
Genetic Insert: sh-mFAK B
Vector Backbone Description: Backbone Marker:Weinberg lab ; Backbone Size:7032; Vector Backbone:pLKO.1 puro (Addgene plasmid 8453); Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19502425
Proper citation: RRID:Addgene_37018 Copy
Species: Homo sapiens
Genetic Insert: perfect complementary binding site for hsa-miR-34a
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19584398
Proper citation: RRID:Addgene_37099 Copy
Species: Mus musculus
Genetic Insert: Foxd3
Vector Backbone Description: Backbone Marker:Stratgene; Backbone Size:2900; Vector Backbone:pBS; Vector Types:In situ hybridization probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9767163
Proper citation: RRID:Addgene_37098 Copy
Species: Rattus norvegicus
Genetic Insert: SypHluorin 4x
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19217377
Proper citation: RRID:Addgene_37005 Copy
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