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Species: Anabaena variabilis
Genetic Insert: Ava3858-3856
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5238; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20813918
Proper citation: RRID:Addgene_45745 Copy
Species: pyrococcus horikoshii
Genetic Insert: PhoRadA precursor
Vector Backbone Description: Backbone Marker:MerckMillipore; Backbone Size:3559; Vector Backbone:pRSF-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23974115
Proper citation: RRID:Addgene_45614 Copy
Species: Nostoc punctiforme
Genetic Insert: NpuDnaEC35-GFP_C
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23974115
Proper citation: RRID:Addgene_45618 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4731; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23661642
Proper citation: RRID:Addgene_45875 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4731; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23661642
Proper citation: RRID:Addgene_45876 Copy
Species: Mus musculus
Genetic Insert: eIF2S2 aa89-331
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11959995
Comments: PCR amplified using EcoRI/BamHI containing primers.
G321D mutation is also present.
Proper citation: RRID:Addgene_45901 Copy
Vector Backbone Description: Backbone Marker:Lidstrom Lab (Addgene plasmid 45826); Backbone Size:7607; Vector Backbone:pCM62; Vector Types:broad host range cloning vector in bacteria; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11495985
Comments: Vector is a broad host range vector (bhr) and is used in the Methylobacterium extorquens AM1.
Proper citation: RRID:Addgene_45827 Copy
Species: Homo sapiens
Genetic Insert: Nck-1 SH2 mutant
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11959995
Comments: Wild type pEGFP-Nck1 (Addgene plasmid 45903) is described in JBC 26:26633-44 2006 (PMID 16835242). Mutations are described in the article linked to this plasmid (PMID 11959995) and then subcloned into the pEGFP-C1 vector using PCR amplification with primers containing XhoI and EcoRI sites.
Proper citation: RRID:Addgene_45893 Copy
Species: Homo sapiens
Genetic Insert: Nck-1 M3 mutant
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11959995
Comments: Wild type pEGFP-Nck1 (Addgene plasmid 45903) is described in JBC 26:26633-44 2006 (PMID 16835242). Mutations are described in the article linked to this plasmid (PMID 11959995) and then subcloned into the pEGFP-C1 vector using PCR amplification with primers containing XhoI and EcoRI sites.
Proper citation: RRID:Addgene_45896 Copy
Species: Homo sapiens
Genetic Insert: hα1Ga
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pDsRed-Express-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The Cav3.1 insert retains its STOP codon, so there is no expression of the Ds-Red. The 5'UTR is gone and contains a new SalI site. The 3' end of insert ends in the endogenous Kpn1 site.
Identification of Cav3.1 is described in Nature. 1998 Feb 26;391(6670):896-900.
Proper citation: RRID:Addgene_45811 Copy
Species: Mus musculus
Genetic Insert: nonmuscle myosin heavy chain II-C, isoform C1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16790446
Proper citation: RRID:Addgene_46041 Copy
Species: Mus musculus
Genetic Insert: nonmuscle myosin heavy chain II-C, isoform C1C2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19240025
Proper citation: RRID:Addgene_46044 Copy
Vector Backbone Description: Backbone Size:7145; Vector Backbone:pOSIP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24050148
Comments: Reference:
St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j
Proper citation: RRID:Addgene_45982 Copy
Species: Homo sapiens
Genetic Insert: human UBI promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4777; Vector Backbone:pDONRP4P1R; Vector Types:pENTR plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21590398
Comments: More information can be found at http://medweb2.unige.ch/salmon/lentilab/lentivectors.html
NOTE: There are some discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These differences should not affect plasmid function.
Proper citation: RRID:Addgene_45959 Copy
Species: Synthetic
Genetic Insert: pTF promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4777; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21761975
Comments: More information can be found at http://medweb2.unige.ch/salmon/lentilab/lentivectors.html
Proper citation: RRID:Addgene_45954 Copy
Genetic Insert: Codon optimized Cas9
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pHSS6; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23709638
Comments: For more information on FlyCRISPR plasmids and a list of related plasmids please refer to: https://www.addgene.org/browse/article/6834/.
Plasmid 51019: pDsRed-attP (www.addgene.org/51019) can be for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site.
Proper citation: RRID:Addgene_45945 Copy
Species: Homo sapiens
Genetic Insert: Eps-15 homology domain-containing protein2
Vector Backbone Description: Backbone Marker:Clontech/ custom-made; Backbone Size:4745; Vector Backbone:pmEGFP-N-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22505029
Proper citation: RRID:Addgene_45934 Copy
Species: Homo sapiens
Genetic Insert: Eps-15 homology domain-containing protein2
Vector Backbone Description: Backbone Marker:Clontech/ custom-made; Backbone Size:4736; Vector Backbone:pmEGFP-N-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22505029
Proper citation: RRID:Addgene_45933 Copy
Species: Homo sapiens
Genetic Insert: Eps-15 homology domain-containing protein2
Vector Backbone Description: Backbone Marker:Clontech/ custom-made; Backbone Size:4736; Vector Backbone:pmEGFP-N-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22505029
Proper citation: RRID:Addgene_45935 Copy
Species: Homo sapiens
Genetic Insert: Yes-kinase associated protein
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2700; Vector Backbone:pENTR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23419361
Proper citation: RRID:Addgene_46050 Copy
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