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Species: Mus musculus
Genetic Insert: Map7-fulllength
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22425998
Proper citation: RRID:Addgene_46076 Copy
Species: Gallus gallus
Genetic Insert: Vcl 884-1066
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: CFP subcloned into 15b/V884-1066 (Addgene plasmid 46176) via a PCR strategy which placed Nde sites at the ends of the CFP cDNA and added the spacer region in EGFPC1/V1-1066 at the Cterminus of CFP.
Proper citation: RRID:Addgene_46180 Copy
Species: Homo sapiens
Genetic Insert: Depdc5
Vector Backbone Description: Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23723238
Proper citation: RRID:Addgene_46336 Copy
Species: Homo sapiens
Genetic Insert: Seh1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23723238
Proper citation: RRID:Addgene_46331 Copy
Species: Homo sapiens
Genetic Insert: PSD95.FingR-eGFP-CCR5TC
Vector Backbone Description: Backbone Size:4113; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23791193
Proper citation: RRID:Addgene_46295 Copy
Species: Homo sapiens
Genetic Insert: GPHN.FingR-eGFP-CCR5TC
Vector Backbone Description: Backbone Size:4113; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23791193
Proper citation: RRID:Addgene_46296 Copy
Species: Homo sapiens
Genetic Insert: Depdc5
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23723238
Proper citation: RRID:Addgene_46327 Copy
Species: Homo sapiens
Genetic Insert: SFPQ
Vector Backbone Description: Backbone Marker:Brown lab (Addgene plasmid 46339); Vector Backbone:pSCT-GAL93-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22966205
Comments: There is a stop codon before HIS tag in the vector.
Proper citation: RRID:Addgene_46320 Copy
Species: Homo sapiens
Genetic Insert: HeLa long myosin light chain kinase
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15020676
Proper citation: RRID:Addgene_46316 Copy
Species: Homo sapiens
Genetic Insert: HeLa long myosin light chain kinase-dead
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15020676
Proper citation: RRID:Addgene_46317 Copy
Species: Mus musculus
Genetic Insert: Vimentin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19726676
Proper citation: RRID:Addgene_46315 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-258 (aka VD1) A50I mutation
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16608855
Comments: A50I mutation introduced into EGFP/V1-258 (Addgene plasmid 46270) by QCPCR.
Proper citation: RRID:Addgene_46271 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-258 (aka VD1)
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16608855
Comments: Nde-Xho fragment from pET15b/Vh1-258 (Addgene plasmid 46173) was subcloned into EGFP/V1-1066 (Addgene plasmid 46265) vector modified to contain unique Nde, Xho sites. Modifications were made by QuikChange PCR to facilitate cloning of cDNAs previously in the pET15b vector. The NdeI site present in the cytomegalovirus promoter region was removed (mutating adenine 234 to thymine), and
a new NdeI site was introduced into the multiple cloning site immediately before the vinculin start codon. Additionally, the 5-XhoI site was removed (mutating cytosine 1345 to adenine), and a 3-XhoI site was introduced 3' to the vinculin cDNA in lieu of a PstI site.
Proper citation: RRID:Addgene_46270 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-851 A50I mutation
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16608855
Comments: A50I mutation introduced into EGFP/V1-851 (Addgene plasmid 46268) by QCPCR mutation.
Proper citation: RRID:Addgene_46269 Copy
Species: Gallus gallus
Genetic Insert: Vcl T12 mutant
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15728584
Comments: T12 mutation introduced by QuikChange PCR to Addgene plasmid 46265.
Mutant inhibits vinculin autoinhibition by ~ 100 fold, vinculin is partially activated and will bind talin, but not actin (unless both talin and actin are present at same time).
Proper citation: RRID:Addgene_46266 Copy
Species: Tomato bushy stunt virus
Genetic Insert: p19
Vector Backbone Description: Backbone Size:4941; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression, pro-siRNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23475073
Proper citation: RRID:Addgene_46306 Copy
Species: Gallus gallus
Genetic Insert: Vcl
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15728584
Comments: full length chicken vinculin (EcoRI fragment of p1005 subcloned into EcoRI-cut pEGFP-C1). ATG at bp 1390-1392. TAA at bp 4588-90. EGFP at N-terminus.
Proper citation: RRID:Addgene_46265 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23870127
Proper citation: RRID:Addgene_46357 Copy
Species: Homo sapiens
Genetic Insert: Anillin
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23870127
Proper citation: RRID:Addgene_46354 Copy
Species: Homo sapiens
Genetic Insert: MRLC2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23870127
Proper citation: RRID:Addgene_46355 Copy
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