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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pET21a-Streptavidin-Alive
 
Resource Report
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10+ mentions
RRID:Addgene_20860 Streptavidin-Alive E. Coli Ampicillin PMID:16554831 Please email [email protected] with your intended use or targets for this plasmid. Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Wt gene 2026-08-15 01:11:57 20
pET21a-Streptavidin-Dead
 
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10+ mentions
RRID:Addgene_20859 Streptavidin-Dead E. Coli Ampicillin PMID:16554831 Please email [email protected] with intended use and/or targets for this plasmid. Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Triple mutant of wt Streptavidin N23A,S27D,S45A 2026-08-15 01:11:57 11
pET21a-BirA
 
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10+ mentions
RRID:Addgene_20857 BirA E. coli Ampicillin PMID:15897449 Please email [email protected] with your intended use and/or targets for this plasmid. Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:57 31
pJF6
 
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RRID:Addgene_21678 LacZ E. coli Ampicillin PMID:1545810 The HindIII fragment of pJF3 containing URA3 UAScycl- and 300 bp of the LEU2 gene promoter and LacZ::CS cut site was inserted into the HindIII site at the upstream end of a promotorless LacZ gene on plasmid JH271. The two LacZ sequences are in direct repeat orientation relative to each other. The lambda sequences are of unknown origin Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature. Backbone Size:0; Vector Backbone:pJH271; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin HO digestion site in one of the LacZ genes 2026-08-15 01:12:04 0
pJY019
 
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RRID:Addgene_32999 etpBAC locus E. coli Ampicillin PMID:16552055 Please note that the His and Myc tags are not in frame with the ORF. In addition, there is a E65G mutation in EtpB. Depositing lab does not think this mutation will affect plasmid function. Backbone Marker:Invitrogen; Backbone Size:4094; Vector Backbone:pBAD/myc-His A; Vector Types:Bacterial Expression, n/a; Bacterial Resistance:Ampicillin 2026-08-15 01:13:45 0
pVS1
 
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1+ mentions
RRID:Addgene_33143 LacO (256 copies) E. coli Ampicillin PMID:18614049 Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable. For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142). pVS2 (Addgene #33144) contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]). pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP. Vector Backbone:Ylplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:47 2
EcMBP165-cpGFP.PPYF.T203V.pRSET
 
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RRID:Addgene_33373 Improved MBP based Maltose Biosensor E. coli Ampicillin PMID:21989929 The GFP-T203V mutation decreases the fluorescence emission of the apo state and increases that of the saturated state in GCaMP2. In the context of MBP165-cpGFP.PPYF, the T203V mutation decreases the fluorescence emission of the apo state by half, whereas saturated fluorescence and affinity are unchanged, increasing ΔF/F to 6.5. Backbone Marker:Invitrogen; Backbone Size:2300; Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin cpGFP146 variant from GCaMP2 inserted between AA165-166 of MBP with T203V mutation in GFP. 2026-08-15 01:13:49 0
pYFJ16-LplA(W37V) (for E. Coli expression)
 
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1+ mentions
RRID:Addgene_34838 LplA W37V E. Coli Ampicillin PMID:20534555 Backbone Marker:Gift from John Cronan; Backbone Size:4800; Vector Backbone:pYFJ16; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Mutated Trp37 to Val 2026-08-15 01:13:55 3
pKSJ155
 
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RRID:Addgene_27126 Colicin Ia + Ia immunity protein E. coli Ampicillin PMID:11004207 This plasmid contains the C-terminal channel forming domain of colicin Ia and the Ia immunity protein. The construct is also referred to as "CT-Medium or CT-M". The SOS promoter for colicin expression is at around residues 148-165 of the insert, in the forward direction. The immunity promoter -35 box is about 460 bp from the end of the insert; there are only about 20 bp between the terminator for the colicin gene, reading in one direction, and the terminator for the immunity protein, reading in the other. Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:00 0
pBB535
 
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1+ mentions
RRID:Addgene_27392 dnaK, dnaJ E. coli Spectinomycin PMID:17565681 Please note that Addgene's sequencing results detect a single nucleotide insertion compared to depositor's sequence in the vector backbone. The plasmid expresses the expected functional protein. Backbone Size:7072; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:13:03 1
pBB528
 
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RRID:Addgene_27390 lacIq E. coli Chloramphenicol PMID:17565681 Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. Backbone Size:4945; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:13:02 0
pMo168
 
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1+ mentions
RRID:Addgene_27389 pMo168 E. coli Kanamycin PMID:19010402 Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp# 5987 and a 5 nucleotide deletion at bp# 6962-6966, when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing scientist, these differences do not affect the function of the plasmid. Backbone Size:7108; Vector Backbone:pBBR1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin A replicative vector for gene expression in Burkholderia 2026-08-15 01:13:03 3
pBB541
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_27394 groESL E. coli Spectinomycin PMID:17565681 Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. Backbone Size:0; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:13:03 2
pDR-GW FLIPglu-170nΔ13V
 
Resource Report
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RRID:Addgene_27999 FLIPglu-170nΔ13V E. Coli Ampicillin PMID:20854260 Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin WT binding protein (no F16A) 2026-08-15 01:13:09 0
pDR-GW FLIPglu-30μΔ13V
 
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RRID:Addgene_27998 FLIPglu-30μΔ13V E. Coli Ampicillin PMID:20854260 Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin D154A in mglB 2026-08-15 01:13:09 0
pSL7 SMR1791
 
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RRID:Addgene_28030 MutS, MutL E. coli Kanamycin PMID:9308969 pSL7 contains a ClaI–BglII fragment of pSL6 (carrying mutS), a BamHI–HindIII fragment of pKC31 (conferring Kanamycin resistance), and a HindIII–PstI fragment of pAL51 (carrying mutL) Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:10 0
pSL5 SMR1713
 
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RRID:Addgene_28028 MutL E. coli Kanamycin PMID:9308969 pSL5 contains a PstI–HindIII fragment of pAL51 carrying E. coli mutL and a KanR-conferring HindIII–BamHI fragment of pKC31 replacing digest sites PstI and BamHI of pBR322 Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:09 0
pDR-GW FLII12Pglu-700μδ6
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_28002 FLII12Pglu-700μδ6 E. Coli Ampicillin PMID:20854260 Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:09 1
pDR-GW FLIPglu-600μΔ13V
 
Resource Report
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RRID:Addgene_28001 FLIPglu-600μΔ13V E. Coli Ampicillin PMID:20854260 Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin F16A in mglB 2026-08-15 01:13:09 0
ClpP-his
 
Resource Report
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RRID:Addgene_22144 ClpP-His6 E. Coli Ampicillin PMID:16237435 NGS results indicate S2P in ClpP Backbone Marker:Qiagen; Backbone Size:3426; Vector Backbone:pQE70; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C-terminal His tag 2026-08-15 01:12:08 0

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