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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pET21a-Streptavidin-Alive Resource Report Resource Website 10+ mentions |
RRID:Addgene_20860 | Streptavidin-Alive | E. Coli | Ampicillin | PMID:16554831 | Please email [email protected] with your intended use or targets for this plasmid. | Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Wt gene | 2026-08-15 01:11:57 | 20 |
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pET21a-Streptavidin-Dead Resource Report Resource Website 10+ mentions |
RRID:Addgene_20859 | Streptavidin-Dead | E. Coli | Ampicillin | PMID:16554831 | Please email [email protected] with intended use and/or targets for this plasmid. | Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Triple mutant of wt Streptavidin N23A,S27D,S45A | 2026-08-15 01:11:57 | 11 |
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pET21a-BirA Resource Report Resource Website 10+ mentions |
RRID:Addgene_20857 | BirA | E. coli | Ampicillin | PMID:15897449 | Please email [email protected] with your intended use and/or targets for this plasmid. | Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:57 | 31 | |
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pJF6 Resource Report Resource Website |
RRID:Addgene_21678 | LacZ | E. coli | Ampicillin | PMID:1545810 | The HindIII fragment of pJF3 containing URA3 UAScycl- and 300 bp of the LEU2 gene promoter and LacZ::CS cut site was inserted into the HindIII site at the upstream end of a promotorless LacZ gene on plasmid JH271. The two LacZ sequences are in direct repeat orientation relative to each other. The lambda sequences are of unknown origin Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature. | Backbone Size:0; Vector Backbone:pJH271; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin | HO digestion site in one of the LacZ genes | 2026-08-15 01:12:04 | 0 |
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pJY019 Resource Report Resource Website |
RRID:Addgene_32999 | etpBAC locus | E. coli | Ampicillin | PMID:16552055 | Please note that the His and Myc tags are not in frame with the ORF. In addition, there is a E65G mutation in EtpB. Depositing lab does not think this mutation will affect plasmid function. | Backbone Marker:Invitrogen; Backbone Size:4094; Vector Backbone:pBAD/myc-His A; Vector Types:Bacterial Expression, n/a; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:45 | 0 | |
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pVS1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_33143 | LacO (256 copies) | E. coli | Ampicillin | PMID:18614049 | Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable. For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142). pVS2 (Addgene #33144) contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]). pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP. | Vector Backbone:Ylplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:47 | 2 | |
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EcMBP165-cpGFP.PPYF.T203V.pRSET Resource Report Resource Website |
RRID:Addgene_33373 | Improved MBP based Maltose Biosensor | E. coli | Ampicillin | PMID:21989929 | The GFP-T203V mutation decreases the fluorescence emission of the apo state and increases that of the saturated state in GCaMP2. In the context of MBP165-cpGFP.PPYF, the T203V mutation decreases the fluorescence emission of the apo state by half, whereas saturated fluorescence and affinity are unchanged, increasing ΔF/F to 6.5. | Backbone Marker:Invitrogen; Backbone Size:2300; Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | cpGFP146 variant from GCaMP2 inserted between AA165-166 of MBP with T203V mutation in GFP. | 2026-08-15 01:13:49 | 0 |
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pYFJ16-LplA(W37V) (for E. Coli expression) Resource Report Resource Website 1+ mentions |
RRID:Addgene_34838 | LplA W37V | E. Coli | Ampicillin | PMID:20534555 | Backbone Marker:Gift from John Cronan; Backbone Size:4800; Vector Backbone:pYFJ16; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Mutated Trp37 to Val | 2026-08-15 01:13:55 | 3 | |
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pKSJ155 Resource Report Resource Website |
RRID:Addgene_27126 | Colicin Ia + Ia immunity protein | E. coli | Ampicillin | PMID:11004207 | This plasmid contains the C-terminal channel forming domain of colicin Ia and the Ia immunity protein. The construct is also referred to as "CT-Medium or CT-M". The SOS promoter for colicin expression is at around residues 148-165 of the insert, in the forward direction. The immunity promoter -35 box is about 460 bp from the end of the insert; there are only about 20 bp between the terminator for the colicin gene, reading in one direction, and the terminator for the immunity protein, reading in the other. | Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:00 | 0 | |
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pBB535 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27392 | dnaK, dnaJ | E. coli | Spectinomycin | PMID:17565681 | Please note that Addgene's sequencing results detect a single nucleotide insertion compared to depositor's sequence in the vector backbone. The plasmid expresses the expected functional protein. | Backbone Size:7072; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:13:03 | 1 | |
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pBB528 Resource Report Resource Website |
RRID:Addgene_27390 | lacIq | E. coli | Chloramphenicol | PMID:17565681 | Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. | Backbone Size:4945; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:13:02 | 0 | |
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pMo168 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27389 | pMo168 | E. coli | Kanamycin | PMID:19010402 | Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp# 5987 and a 5 nucleotide deletion at bp# 6962-6966, when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing scientist, these differences do not affect the function of the plasmid. | Backbone Size:7108; Vector Backbone:pBBR1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | A replicative vector for gene expression in Burkholderia | 2026-08-15 01:13:03 | 3 |
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pBB541 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27394 | groESL | E. coli | Spectinomycin | PMID:17565681 | Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. | Backbone Size:0; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:13:03 | 2 | |
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pDR-GW FLIPglu-170nΔ13V Resource Report Resource Website |
RRID:Addgene_27999 | FLIPglu-170nΔ13V | E. Coli | Ampicillin | PMID:20854260 | Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | WT binding protein (no F16A) | 2026-08-15 01:13:09 | 0 | |
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pDR-GW FLIPglu-30μΔ13V Resource Report Resource Website |
RRID:Addgene_27998 | FLIPglu-30μΔ13V | E. Coli | Ampicillin | PMID:20854260 | Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | D154A in mglB | 2026-08-15 01:13:09 | 0 | |
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pSL7 SMR1791 Resource Report Resource Website |
RRID:Addgene_28030 | MutS, MutL | E. coli | Kanamycin | PMID:9308969 | pSL7 contains a ClaI–BglII fragment of pSL6 (carrying mutS), a BamHI–HindIII fragment of pKC31 (conferring Kanamycin resistance), and a HindIII–PstI fragment of pAL51 (carrying mutL) | Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:10 | 0 | |
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pSL5 SMR1713 Resource Report Resource Website |
RRID:Addgene_28028 | MutL | E. coli | Kanamycin | PMID:9308969 | pSL5 contains a PstI–HindIII fragment of pAL51 carrying E. coli mutL and a KanR-conferring HindIII–BamHI fragment of pKC31 replacing digest sites PstI and BamHI of pBR322 | Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:09 | 0 | |
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pDR-GW FLII12Pglu-700μδ6 Resource Report Resource Website 1+ mentions |
RRID:Addgene_28002 | FLII12Pglu-700μδ6 | E. Coli | Ampicillin | PMID:20854260 | Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:09 | 1 | ||
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pDR-GW FLIPglu-600μΔ13V Resource Report Resource Website |
RRID:Addgene_28001 | FLIPglu-600μΔ13V | E. Coli | Ampicillin | PMID:20854260 | Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | F16A in mglB | 2026-08-15 01:13:09 | 0 | |
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ClpP-his Resource Report Resource Website |
RRID:Addgene_22144 | ClpP-His6 | E. Coli | Ampicillin | PMID:16237435 | NGS results indicate S2P in ClpP | Backbone Marker:Qiagen; Backbone Size:3426; Vector Backbone:pQE70; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C-terminal His tag | 2026-08-15 01:12:08 | 0 |
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