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  • Organism:mus musculus (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pcDNA3 NT3
 
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RRID:Addgene_39981 Neurotrophin 3 Mus musculus Ampicillin Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:32 1
pGEX4T-1 Slp2-a C2AB
 
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RRID:Addgene_40058 Slp2-a C2AB Mus musculus Ampicillin PMID:22820376 Backbone Marker:Promega; Backbone Size:4900; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C2AB domains only 2026-08-15 01:14:33 0
pEGFP-C1 Slp2-a E11A/R32A
 
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RRID:Addgene_40055 Slp2-a E11A/R32A Mus musculus Kanamycin PMID:22820376 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Glutamate 11 to Alanine, Arginine 32 to Alanine 2026-08-15 01:14:33 0
pEGFP-C1 Slp2-a C2B
 
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RRID:Addgene_40053 Slp2-a C2B Mus musculus Kanamycin PMID:22820376 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin C2B domain 2026-08-15 01:14:33 0
pEGFP-C1 Slp2-a C2A
 
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RRID:Addgene_40052 Slp2-a C2A Mus musculus Kanamycin PMID:22820376 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin C2A domains 2026-08-15 01:14:33 0
Ubc-megf10-GFP
 
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RRID:Addgene_40207 Megf10 Mus musculus Ampicillin PMID:22407321 Vector Backbone:pUb-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:34 2
CD40L-MIEG-hCD4 (hCD4-CD40L)
 
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RRID:Addgene_40355 CD40L Mus musculus Ampicillin PMID:19405121 The CD40-ligand (CD40L)-expressing retroviral vector was constructed by PCR amplification of cDNA from anti-CD3 activated mouse T cells with the following primers for CD40-ligand: sense 5'-CTTTCAGTCAGCATGATAGAAACA-3' and antisense 5'-TCAGAGTTTGAGTAAGCCAAAAGA-3'; these primers were used to amplify the complete coding region of mouse CD40 ligand. The CD40-ligand gene was then reamplified with primers containing XhoI and NotI sites and then cloned via XhoI and NotI sites into a retroviral vector expressing human CD4. Backbone Marker:Addgene plasmid 40569; Vector Backbone:MIEG-hCD4; Vector Types:Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:35 2
pMCP-MU-Luc (MCP-1 mut promoter in pGL3-basic)
 
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RRID:Addgene_40325 MCP-1 promoter (mutated) Mus musculus Ampicillin PMID:10973278 Note: Addgene NGS results identified an extra ~500 bp region upstream of the expected promoter, which contains a CMV enhancer feature. It is unknown what impact the region may have on plasmid function, but the extra sequence appears to have been present in the original sample received by Addgene. A plasmid containing the mouse MCP-1 promoter driving luciferase (pMCP-Luc) was constructed from pGL3-basic (Promega, Madison, WI) and a PCR-generated MCP-1 promoter fragment. A proofreading competent Taq reagent (AccuTaq, Sigma) was used to ensure high fidelity PCR. 1.2 kb of the MCP-1 promoter (GenBank Accession no. U12470) was obtained by PCR of C57BL/6 mouse DNA with the following primers: 5′–TCATGTATATGGCTTTCCAGGTCT–3′ (sense strand, distal to transcription start) 5′–TGCACTTCTGGCTGCTCTGAG GCA–3′ (antisense strand, proximal to transcription start) The PCR product terminates 28-bp downstream of the MCP-1 TATA site. XmaI and XhoI sites were added to the MCP-1 promoter by a second round of PCR with the primers: 5′–ATATCACCCGGGTCATGTATATGGCTTTCCAGGTCT–3′ 5′–TAGATTCTCGAGTGCACTTCTGGCTGCTCTGAGGCA–3′ and XmaI and XhoI were used to clone the MCP-1 promoter fragment into pGL3-basic. For the construction of the MCP-1 mutant promoter, the BCL-6 site was mutated by a two step PCR–based strategy using the above flanking primers and the following complementary primers to mutate the BCL-6 site: 5′–TCTCTCTTCCACTTCCT[TT]AAACA–3′ 5′–TGTTT[AA]AGGAAGTGGAAGAGAGA–3′ (mutated bases are in brackets) The mutant promoter was cloned into pGL3 via XhoI and XmaI. The wild-type and mutant MCP-1 promoter sequences were verified by sequencing. Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin contains MCP-1 promoter region through the nucleotides 28-bp downstream of the MCP-1 TATA site. Sequence has two base changes from the wild-type MCP-1 promoter around the -150 bp position (see note below). 2026-08-15 01:14:35 0
2C::tdTomato Reporter
 
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RRID:Addgene_40281 2C Promoter (2-cell stage promoter) Mus musculus Ampicillin PMID:22722858 The CMV promoter from the parent pcDNA plasmid was removed with MluI and HindIII, leaving approx. 6400kb backbone. The 2C promoter was put in its place (730bp) using the same sites. Hence, the full plasmid is 7129bp. The promoter is active shortly after fertilization of mouse embryos to about the 8-cell stage. It is also on in a subset of MESCs & miPSCs. Backbone Marker:Invitrogen/Life Tech; Backbone Size:6400; Vector Backbone:pcDNA Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:35 15
FUGW-H1-Syndecan-1 shRNA (UTR)
 
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RRID:Addgene_40623 Syndecan-1 shRNA(UTR) Mus musculus Ampicillin PMID:22936997 This shRNA plasmid was generated by inserting the hairpin oligonucleotides into the FUGW-H1 lentiviral construct. Backbone Size:10130; Vector Backbone:FUGW-H1; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:14:38 2
AMPK-γ1-HA
 
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RRID:Addgene_40605 AMPK-γ1 Mus musculus Kanamycin PMID:17012231 AMPK-γ1 insert has Kozak sequence embedded at 5'-terminus and a stop codon at the 3'-terminus. The FLAG of the pCMV-Tag4a backbone is out of frame. Backbone Size:4300; Vector Backbone:pCMV-Tag4A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:14:37 1
AMPK-β2-FLAG
 
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RRID:Addgene_40603 AMPK-β2 Mus musculus Kanamycin PMID:17012231 Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag4A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:14:37 1
AMPK-β1-FLAG
 
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RRID:Addgene_40602 AMPK-β1 Mus musculus Kanamycin PMID:17012231 Backbone Size:4300; Vector Backbone:pCMV-Tag4A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:14:37 0
AMPK-β2-MYC
 
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RRID:Addgene_40606 AMPK-β2 Mus musculus Kanamycin PMID:17012231 Does not express well. Minimal kozak only GCCATGGG. Backbone Size:4300; Vector Backbone:pCMV-Tag5a; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:14:37 0
pEMB44 / Mouse_LRRK2_1_342
 
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RRID:Addgene_40555 Lrrk2 Mus musculus Ampicillin Backbone Marker:Emerald; Vector Backbone:pEMB44; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin WT 2026-08-15 01:14:37 0
p3.5VPIII.EGFP.IGR2.1
 
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RRID:Addgene_40865 Mouse vasopressin gene Mus musculus Ampicillin PMID:12944509 the following mutations are known and do not effect plasmid function: TT--5510CTTG, G5386A, A4984T,A6794G. Backbone Size:4000; Vector Backbone:pSE280; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:39 1
FUW-TetO-Dppa2
 
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RRID:Addgene_40799 Dppa2 Mus musculus Ampicillin PMID:22980981 Backbone Size:8339; Vector Backbone:FUW-TetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:39 0
FUW-TetO-Esrrb
 
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RRID:Addgene_40798 Esrrb Mus musculus Ampicillin PMID:22980981 Backbone Size:8339; Vector Backbone:FUW-TetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:38 4
FUW-TetO-Ctcf
 
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RRID:Addgene_40801 Ctcf Mus musculus Ampicillin PMID:22980981 Backbone Size:8339; Vector Backbone:FUW-TetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:39 1
pDEST47-Arl13b17
 
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RRID:Addgene_40875 Arl13b Mus musculus Ampicillin PMID:21976698 In Larkins et al (2011), this is plasmid "Arf domain GFP tagged" or "Arl domain-GFP" as referenced in Figure 2. Amino acids 1-199 of Arl13b were Gateway cloned into pcDNA-DEST47. This effectively deleted the novel C-terminal domain. Backbone Marker:Invitrogen; Backbone Size:7780; Vector Backbone:pcDNA-DEST47; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin contains Arl13b amino acids 1-199 2026-08-15 01:14:39 0

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