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pMEL15_MATz
 
Resource Report
Resource Website 		RRID:Addgene_166099 MAT Z1 Saccharomyces cerevisiae Ampicillin Addgene QC identified differences to the theoretical sequence that the depositing lab does not believe are of functional concern. Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t, Daran Lab; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_GAL2
 
Resource Report
Resource Website 		RRID:Addgene_166088 GAL2 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrXII:291472..291491 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_CUP1-1
 
Resource Report
Resource Website 		RRID:Addgene_166086 CUP1-1 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrVIII:212613..212632 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_MET16
 
Resource Report
Resource Website 		RRID:Addgene_166091 MET16 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrXVI:877523..877542 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome. Addgene QC identified several discrepancies compared to the theoretical reference. Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_RPL22A
 
Resource Report
Resource Website 		RRID:Addgene_166079 Promoter of RPL22A Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrXII:263006..263025 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome. Addgene QC identified discrepancies compared to the theoretical sequence. The depositing lab confirms the functional activity of the construct despite these differences. Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:33 0
pNSU299_RPL39
 
Resource Report
Resource Website 		RRID:Addgene_166078 Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrX: 75914-75933 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_SLA1
 
Resource Report
Resource Website 		RRID:Addgene_166075 SLA1 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrII: 213734-213753 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_RRT8
 
Resource Report
Resource Website 		RRID:Addgene_166080 Promoter of RRT8 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrXV:241445..241464 (-strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
HCP1
 
Resource Report
Resource Website 		RRID:Addgene_166103 Msn2-sgRNA#27 Saccharomyces cerevisiae Kanamycin Vector Backbone:pYTK101; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:09:34 0
pROS15_MATz
 
Resource Report
Resource Website 		RRID:Addgene_166100 MAT Z1 Saccharomyces cerevisiae Ampicillin Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t, Daran Lab; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
HCP3
 
Resource Report
Resource Website 		RRID:Addgene_166105 Msn2-sgRNA#27 Saccharomyces cerevisiae Kanamycin Vector Backbone:pYTK103; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:09:34 0
deltaVP1 only
 
Resource Report
Resource Website 		RRID:Addgene_166675 Murine polyomavirus deltaVP1 (NLS deletion mutant) Saccharomyces cerevisiae Ampicillin PMID:34591448 Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:09:37 0
pLIB-HIS-TEV-scCAK1
 
Resource Report
Resource Website 		RRID:Addgene_177014 CAK1 cyclin-dependent protein kinase-activating kinase CAK1 Saccharomyces cerevisiae Ampicillin PMID:34791727 Backbone Size:5022; Vector Backbone:pLIB-HIS-TEV; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:04 0
YIplac211-NHX1-iGFP
 
Resource Report
Resource Website 		RRID:Addgene_177108 NHX1 Saccharomyces cerevisiae Ampicillin PMID:34739034 Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:05 0
YIplac211-ANP1-msGFP2
 
Resource Report
Resource Website 		RRID:Addgene_177100 ANP1 Saccharomyces cerevisiae Ampicillin PMID:34739034 Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:05 0
YIplac211-MNN9-msGFP2
 
Resource Report
Resource Website 		RRID:Addgene_177105 MNN9 Saccharomyces cerevisiae Ampicillin PMID:34739034 Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:05 0
YIplac211-NHX1-HaloTagx2
 
Resource Report
Resource Website 		RRID:Addgene_177107 NHX1 Saccharomyces cerevisiae Ampicillin PMID:34739034 Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:05 0
YIplac211-msGFP2-STE13
 
Resource Report
Resource Website 		RRID:Addgene_177106 STE13 Saccharomyces cerevisiae Ampicillin PMID:34739034 Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:05 0
pRS306Sup35R1+6
 
Resource Report
Resource Website 		RRID:Addgene_17719 Sup35R1+6 Saccharomyces cerevisiae Ampicillin PMID:17893150 Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:06 0
pRS306Sup35R1+8
 
Resource Report
Resource Website 		RRID:Addgene_17721 Sup35R1+8 Saccharomyces cerevisiae Ampicillin PMID:17893150 Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:06 0

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