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Species: HPV
Genetic Insert: HPV 5 E7
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-C FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37889 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BARF1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Though there are some discrepancies between the depositor sequence and Addgene's quality control sequence, they are not known to affect plasmid function.
Proper citation: RRID:Addgene_37922 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BBLF1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37923 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BcRF1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37924 Copy
Species: HPV
Genetic Insert: HPV 18E6
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37884 Copy
Species: HPV
Genetic Insert: HPV 18E6 no*
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37885 Copy
Species: HPV
Genetic Insert: HPV 18 E7
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-C FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37886 Copy
Species: HPV
Genetic Insert: HPV 8 E7
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-C FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: The 8E7 template was given to us by Harold Pfister who originally cloned the HPV8 genome. (J. Virology 1986, Pfister H. et al.)
Proper citation: RRID:Addgene_37920 Copy
Species: HPV
Genetic Insert: HPV 16 E7
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [STFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37881 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BDLF3.5
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37929 Copy
Species: Homo sapiens
Genetic Insert: EB1 shRNA #3
Vector Backbone Description: Backbone Marker:Addgene plasmid 8453; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22483942
Proper citation: RRID:Addgene_37927 Copy
Species: Homo sapiens
Genetic Insert: EB1 shRNA #1
Vector Backbone Description: Backbone Marker:Addgene plasmid 8453; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22483942
Proper citation: RRID:Addgene_37928 Copy
Species: HPV
Genetic Insert: HPV 11 E6
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-C FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37873 Copy
Genetic Insert: HPV 16 E5
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37874 Copy
Species: HPV
Genetic Insert: HPV 8 E6
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-C FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37919 Copy
Species: HPV
Genetic Insert: HPV 6b E5b
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37914 Copy
Species: Strongylocentrotus purpuratus
Genetic Insert: ABCB1a
Vector Backbone Description: Backbone Size:4851; Vector Backbone:pCS2+; Vector Types:Sea urchin, zebrafish, Xenopus ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124201
Proper citation: RRID:Addgene_38036 Copy
Species: Homo sapiens
Genetic Insert: Nucleoporin 98
Vector Backbone Description: Backbone Size:2968; Vector Backbone:pPEP-TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21685911
Comments: see also:
Strelkov, S. V., Kreplak, L., Herrmann, H. & Aebi, U. (2004). Methods Cell Biol.78, 25–43.
Proper citation: RRID:Addgene_38037 Copy
Species: Homo sapiens
Genetic Insert: shREV3-5
Vector Backbone Description: Backbone Marker:oligoengine; Backbone Size:4354; Vector Backbone:pSUPERIOR.puro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22028621
Proper citation: RRID:Addgene_38030 Copy
Species: Homo sapiens
Genetic Insert: shREV3-4
Vector Backbone Description: Backbone Marker:Trono Laboratory; Backbone Size:11085; Vector Backbone:pLVTHM; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22028621
Comments: A versatile tool for conditional gene expression and knockdown.
Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P.
Nat Methods. 2006 Feb;3(2):109-16.
PMID:16432520
Proper citation: RRID:Addgene_38033 Copy
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