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Species: Saccharomyces cerevisiae
Genetic Insert: BRR2
Vector Backbone Description: Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2017
Proper citation: RRID:Addgene_90256 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pZ-SIC1
Vector Backbone Description: Backbone Size:6559; Vector Backbone:pNH604; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Proper citation: RRID:Addgene_90408 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90305 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90304 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90307 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90306 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90302 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Vector Backbone:pDEST15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2016
Proper citation: RRID:Addgene_90264 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BRR2
Vector Backbone Description: Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2017
Proper citation: RRID:Addgene_90263 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BRR2
Vector Backbone Description: Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2017
Proper citation: RRID:Addgene_90260 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BRR2
Vector Backbone Description: Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072132
Comments: Ledoux and Guthrie, JBC, 2017
Proper citation: RRID:Addgene_90261 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUB2
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11156604
Comments: Addgene NGS results found E16G, H60Y, A114T, K202E, E222G, and E267G in the SUB2 translation compared to NP_010199.1
Proper citation: RRID:Addgene_90455 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:3882; Vector Backbone:2 micron; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: For more information see https://benchling.com/pub/ellis-crispr-tools
Proper citation: RRID:Addgene_90519 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Yeast sgRNA expression vector
Vector Backbone Description: Backbone Size:1753; Vector Backbone:N/A; Vector Types:Yeast Expression, CRISPR, Yeast gap repair; Bacterial Resistance:Ampicillin
Comments: Refer to https://benchling.com/pub/ellis-crispr-tools for guidelines on how to use
Proper citation: RRID:Addgene_90516 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3936; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27539380
Proper citation: RRID:Addgene_79993 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: WHI2
Vector Backbone Description: Backbone Size:6568; Vector Backbone:pRS316/pAG36; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30498022
Proper citation: RRID:Addgene_80589 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HYGMX
Vector Backbone Description: Backbone Size:8690; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: The plasmid contains HO sequence with its endogenous promoter. Can be used for mating type switch, diploidization.
Please note the Addgene verified sequence differs from the original diagram map.
Proper citation: RRID:Addgene_81037 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NATMX - HO endonuclease
Vector Backbone Description: Backbone Size:8690; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: The plasmid contains HO sequence with its endogenous promoter. Can be used for mating type switch, diploidization.
Proper citation: RRID:Addgene_81038 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: U6 RNA with U100C_U101C mutation
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27484481
Proper citation: RRID:Addgene_81236 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: U6 RNA with A34G/A35G/G96C/A97C mutation
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27484481
Proper citation: RRID:Addgene_81238 Copy
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