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Vector Backbone Description: Vector Backbone:pSynORI; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41629321
Comments: J23119-Repressor (LS)-parDE-parCBA-RNA Primer (-68nt)-Target (LS)-J23110-RFP-CmR
Proper citation: RRID:Addgene_253201 Copy
Vector Backbone Description: Vector Backbone:pSynORI; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41629321
Comments: J23119-Repressor (LS)-RNA Primer (-68nt)-Target (LS)-J23110-parDE-parCBA-CmR
Proper citation: RRID:Addgene_253204 Copy
Species: Unspecified
Genetic Insert: Spacer
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41535170
Proper citation: RRID:Addgene_254021 Copy
Species: Lambda phage (Addgene #87359)
Genetic Insert: lambdaRED
Vector Backbone Description: Backbone Size:3008; Vector Backbone:CloDF13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42067623
Proper citation: RRID:Addgene_253997 Copy
Species: Synthetic
Genetic Insert: cat
Vector Backbone Description: Vector Backbone:pR6K; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40927394
Proper citation: RRID:Addgene_243900 Copy
Species: Synthetic
Genetic Insert: tetA
Vector Backbone Description: Vector Backbone:pR6K; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40927394
Proper citation: RRID:Addgene_243901 Copy
Species: Other
Genetic Insert: J23119 CAO1 target + GAPDH T17 5x array non-targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253628 Copy
Species: Other
Genetic Insert: J23119 GAPDH T17 target + GAPDH T17 5x array targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253629 Copy
Species: Other
Genetic Insert: J23119 GAPDH T17 target + CAO1 5x array non-targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253630 Copy
Species: Other
Genetic Insert: PxylA
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235411 Copy
Species: Other
Genetic Insert: sfGFP Dropout Cassette
Vector Backbone Description: Vector Backbone: pNW33N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235362 Copy
Species: Other
Genetic Insert: Bba_B0015
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235369 Copy
Species: Other
Genetic Insert: S718 term
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235439 Copy
Species: Synthetic
Genetic Insert: mEBSn-mini35S (A1-B1)
Vector Backbone Description: Vector Backbone:pUPD2; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40976865
Proper citation: RRID:Addgene_254063 Copy
Species: E. coli
Genetic Insert: A1-amtR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2cd34 parts are expression parts in the CTK system. They just need promoters to be fully functional
Proper citation: RRID:Addgene_248497 Copy
Species: E. coli
Genetic Insert: B1-bm3R1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2cd34 parts are expression parts in the CTK system. They just need promoters to be fully functional
Proper citation: RRID:Addgene_248498 Copy
Species: E. coli
Genetic Insert: B2-bm3R1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2cd34 parts are expression parts in the CTK system. They just need promoters to be fully functional
Proper citation: RRID:Addgene_248499 Copy
Species: E. coli
Genetic Insert: L3S2P55
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 4 pasts are terminators in the CTK system
Proper citation: RRID:Addgene_248471 Copy
Vector Backbone Description: Backbone Size:4047; Vector Backbone:pSpy3C; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white.
Resource Information: To create pSpy3C, we took the minimal pWV01* replicon from pBAV1K-T5-gfp (Plasmid #26702), the chloramphenicol resistance cassette from pIB166 (Plasmid #90189) and the MCS featuring the Plac_mrfp cassette from pBS3Clux (#55172).
Please download the file linked below to obtain a well annotated map of the plasmid.
Proper citation: RRID:Addgene_220253 Copy
Genetic Insert: sgRNA - cas9
Vector Backbone Description: Backbone Size:6603; Vector Backbone:pNORg10; Vector Types:E. coli - R. gnavus shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41820342
Comments: Please visit https://doi.org/10.1101/2025.08.12.669022 for bioRxiv preprint.
Proper citation: RRID:Addgene_249345 Copy
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