Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
p663-UBC-miniTurbo-V5-RNASEL_IDG-K Resource Report Resource Website |
RRID:Addgene_170604 | miniTurbo-V5-RNASEL | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | ||
|
p663-UBC-miniTurbo-V5-RPS6KL1_IDG-K Resource Report Resource Website |
RRID:Addgene_170605 | miniTurbo-V5-RPS6KL1 | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:09 | 0 | ||
|
pFLAG R106L-CPTP Resource Report Resource Website |
RRID:Addgene_170744 | CPTP | Homo sapiens | Ampicillin | PMID:23863933 | Vector Backbone:pFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | R106L | 2026-08-15 01:10:11 | 0 | |
|
pFLAG CPTP Resource Report Resource Website |
RRID:Addgene_170742 | CPTP | Homo sapiens | Ampicillin | PMID:21625605 | Vector Backbone:pFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | ||
|
pFLAG GLTP Resource Report Resource Website |
RRID:Addgene_170740 | GLTP | Homo sapiens | Ampicillin | PMID:21625605 | Vector Backbone:pFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | ||
|
pFUGW-FIRE-pHLy Resource Report Resource Website 1+ mentions |
RRID:Addgene_170774 | LAMP1 | Homo sapiens | Ampicillin | PMID:34102054 | Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318. Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/ Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318 | Backbone Marker:Addgene (Plasmid #14883); Backbone Size:9955; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 84 nucleotide Signal Sequence switched to the beginning of the construct | 2026-08-15 01:10:11 | 5 |
|
pLJM1-FIRE-pHLy Resource Report Resource Website 1+ mentions |
RRID:Addgene_170775 | LAMP1 | Homo sapiens | Ampicillin | PMID:34102054 | Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318. Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/ Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318 | Backbone Marker:Addgene Plasmid #19319; Backbone Size:8083; Vector Backbone:pLJM1-EGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 84 nucleotide Signal Sequence switched to the beginning of the construct | 2026-08-15 01:10:10 | 4 |
|
AICSDP-116:PCNA-mEGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_170818 | PCNA Homology Arms with mEGFP-linker | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human PCNA in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GEGQGQGQGPGRGYAYRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used, and WTC-11 specific homozygous SNP(s) | 2026-08-15 01:10:11 | 1 | |
|
pmEmerald-ERGIC-53 Resource Report Resource Website 1+ mentions |
RRID:Addgene_170717 | ERGIC-53 | Homo sapiens | Kanamycin | PMID:35051356 | Please see the Genbank file under Resource Information for an annotated sequence file from the depositing laboratory. | Backbone Marker:Clontech; Backbone Size:4026; Vector Backbone:pDendra2-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:10 | 3 | |
|
pBR-Lent-STAT5A-CA Resource Report Resource Website 1+ mentions |
RRID:Addgene_170662 | STAT5A | Homo sapiens | Ampicillin | PMID:34263728 | A constitutively active form of STAT5A was cloned into the lentiviral expression vector. | Vector Backbone:pTRIPZ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | H298R and S710F | 2026-08-15 01:10:10 | 1 |
|
pET30 W96A-GLTP Resource Report Resource Website |
RRID:Addgene_170734 | W96A-GLTP | Homo sapiens | Kanamycin | PMID:15329726 | Vector Backbone:pET30 Xa/LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | W96A | 2026-08-15 01:10:10 | 0 | |
|
pSUMO GLTP Resource Report Resource Website |
RRID:Addgene_170732 | GLTP | Homo sapiens | Kanamycin | PMID:30206120 | Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:10 | 0 | ||
|
pET30 GLTP Resource Report Resource Website |
RRID:Addgene_170733 | GLTP | Homo sapiens | Kanamycin | PMID:15329726 | Vector Backbone:pET30 Xa/LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:11 | 0 | ||
|
pSUMO K60A-CPTP Resource Report Resource Website |
RRID:Addgene_170736 | K60A-CPTP | Homo sapiens | Kanamycin | PMID:23863933 | Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | K60A | 2026-08-15 01:10:11 | 0 | |
|
pRSFDuet1-His-TEV-OTC_d1_Ub Resource Report Resource Website |
RRID:Addgene_170729 | E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin | Homo sapiens | Kanamycin | PMID:33719392 | Backbone Marker:Novagen (+ 6xHis-TEV in-house modification); Backbone Size:3810; Vector Backbone:pRSFDuet1-6xHis-TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | The C-terminus of OTC was truncated by removing one amino acid. | 2026-08-15 01:10:11 | 0 | |
|
dGFP-Granuphilin Resource Report Resource Website |
RRID:Addgene_170868 | Synaptotagmin-like 5 | Homo sapiens | Kanamycin | PMID:34172739 | Backbone Marker:Clonetech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:11 | 0 | ||
|
pCMV-GABRA1-IRES2-EGFP Resource Report Resource Website |
RRID:Addgene_170820 | Human GABAA receptor alpha1 subunit | Homo sapiens | Kanamycin | PMID:30481215 | Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:11 | 0 | ||
|
pCMV-GABRA1(A322D)-IRES2-EGFP Resource Report Resource Website |
RRID:Addgene_170821 | Human GABAA receptor alpha1 subunit carrying A322D mutation | Homo sapiens | Kanamycin | PMID:30481215 | Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | change Alanine 322 to Aspartate | 2026-08-15 01:10:11 | 0 | |
|
pRF TpC RE8 Resource Report Resource Website |
RRID:Addgene_171047 | RE8 | Homo sapiens | Ampicillin | PMID:33852895 | Vector Backbone:pRF TpC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:13 | 0 | ||
|
pRF TpC RE7 Resource Report Resource Website |
RRID:Addgene_171046 | RE7 | Homo sapiens | Ampicillin | PMID:33852895 | Vector Backbone:pRF TpC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:12 | 0 |
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
