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Species: Homo sapiens
Genetic Insert: FOXO3a
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11964479
Comments: Mutations in phosphorylation sites and deletion of transactivation domain.
Proper citation: RRID:Addgene_1797 Copy
Genetic Insert: pmyc1tetO-gfp, tetR(B)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: Expresses GFP under control of tetR(B). Also see Pubmed IDs 18059281 and 15687379.
pUV15 derivative, Genotype: Hygr, Kmr, pimyc-tetR, pmyc1 tetO-gfp+
Proper citation: RRID:Addgene_17975 Copy
Species: Homo sapiens
Genetic Insert: TAF4
Vector Backbone Description: Backbone Size:4700; Vector Backbone:na; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8942982
Proper citation: RRID:Addgene_17983 Copy
Species: Homo sapiens
Genetic Insert: Osa2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCGT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12200431
Proper citation: RRID:Addgene_17987 Copy
Species: Homo sapiens
Genetic Insert: SF2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10766243
Proper citation: RRID:Addgene_17990 Copy
Species: Homo sapiens
Genetic Insert: heterochromatin protein alpha
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30907250
Comments: NheI-BglII-digested PCR products pREST-B-mCherry into the corresponding sites of the vector backbone of pEGFP-C1
Proper citation: RRID:Addgene_179703 Copy
Species: Homo sapiens
Genetic Insert: Wnt-3
Vector Backbone Description: Backbone Size:6620; Vector Backbone:pLNC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Gavin, B.J., McMahon, J.A., and McMahon, A.P. Expression of multiple novel Wnt-1/Int-1-related genes during fetal and adult mouse development. Genes Dev.,4:2319-2332, 1990.
Roelink, H., and Nusse, R. Expression of two members of the Wnt family during mouse development -restricted temporal and spatial patterns in the developing neural tube. Genes Dev., 5: 381-388, 1991.
Proper citation: RRID:Addgene_17993 Copy
Species: Homo sapiens
Genetic Insert: hTAF1
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7680771
Comments: See author's map for more information. A BamHI/KpnI fragment from pHAX250 (pCMVHAX hTAFII250) was cloned into BamHI/StuI cut pCS2+.
Proper citation: RRID:Addgene_17997 Copy
Species: Neisseria lactamica
Genetic Insert: NlaCas3c
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5203; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051351
Proper citation: RRID:Addgene_180214 Copy
Species: Synthetic
Genetic Insert: PEmax-P2A-EGFP
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34653350
Proper citation: RRID:Addgene_180020 Copy
Species: Homo sapiens
Genetic Insert: Human LRP6
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4092; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33299006
Proper citation: RRID:Addgene_180143 Copy
Species: Homo sapiens
Genetic Insert: Mitochondria-targeted Bcl-2
Vector Backbone Description: Vector Backbone:pEMD-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11546793
Proper citation: RRID:Addgene_18001 Copy
Species: Homo sapiens
Genetic Insert: hTRF2
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pLPC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11923537
Comments: {Wang et al., 2004, Cell, 119, 355-68}
Proper citation: RRID:Addgene_18008 Copy
Species: Homo sapiens
Genetic Insert: hTRF2
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pWZL Hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11923537
Proper citation: RRID:Addgene_18012 Copy
Species: Homo sapiens
Genetic Insert: hTRF2
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pWZL Hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11923537
Proper citation: RRID:Addgene_18013 Copy
Species: E. coli
Genetic Insert: FLIPglu D154A Delta13
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20354141
Proper citation: RRID:Addgene_18015 Copy
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35145312
Comments: Digest vector with AgeI+NheI to clone any sequence between the ribozymes
Proper citation: RRID:Addgene_180184 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*01:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00001 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180448 Copy
Genetic Insert: gRNAscr
Vector Backbone Description: Backbone Size:6487; Vector Backbone:pLenti_puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34588447
Proper citation: RRID:Addgene_180426 Copy
Species: Mus musculus
Genetic Insert: zif268
Vector Backbone Description: Backbone Size:3700; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18332042
Proper citation: RRID:Addgene_18045 Copy
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