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Genetic Insert: 2-input A then B;0-mKate2-sfGFP
Vector Backbone Description: Vector Backbone:pSB4K5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32958811
Proper citation: RRID:Addgene_126532 Copy
Genetic Insert: 0-BFP-mKate2-sfGFP
Vector Backbone Description: Vector Backbone:pSB4K5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32958811
Proper citation: RRID:Addgene_126535 Copy
Genetic Insert: 2-input A then B;BFP-0-sfGFP
Vector Backbone Description: Vector Backbone:pSB4K5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32958811
Proper citation: RRID:Addgene_126533 Copy
Genetic Insert: 2-input A then B;BFP-mKate2-sfGFP
Vector Backbone Description: Vector Backbone:pSB4K5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32958811
Proper citation: RRID:Addgene_126534 Copy
Genetic Insert: 0-0-mKate2-sfGFP
Vector Backbone Description: Vector Backbone:pSB4K5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32958811
Proper citation: RRID:Addgene_126538 Copy
Species: Rhodobacter sphaeroides
Genetic Insert: tyrosine ammonia lyase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3593; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31117389
Proper citation: RRID:Addgene_126524 Copy
Genetic Insert: 2-input B then A; 0-0-GFP226
Vector Backbone Description: Vector Backbone:pSB4K5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32958811
Proper citation: RRID:Addgene_126529 Copy
Species: Homo sapiens
Genetic Insert: RAB9 (Homo sapiens)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12070301
Proper citation: RRID:Addgene_12663 Copy
Species: Homo sapiens
Genetic Insert: RAB7 (Homo sapiens)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pDsRed-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12070301
Proper citation: RRID:Addgene_12662 Copy
Vector Backbone Description: Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126605 Copy
Vector Backbone Description: Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126603 Copy
Genetic Insert: Draper cytoplasmic domain
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30139739
Proper citation: RRID:Addgene_126670 Copy
Genetic Insert: Draper cytoplasmic ITAM residues mutated to Phe
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30139739
Proper citation: RRID:Addgene_126671 Copy
Genetic Insert: Src42a
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30139739
Proper citation: RRID:Addgene_126674 Copy
Species: Homo sapiens
Genetic Insert: RAB11A (Homo sapiens)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pDsRed-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12070301
Proper citation: RRID:Addgene_12680 Copy
Species: Homo sapiens
Genetic Insert: RAB11A (Homo sapiens)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pDsRed-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12070301
Proper citation: RRID:Addgene_12679 Copy
Species: Homo sapiens
Genetic Insert: RAB9 (Homo sapiens)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:p-DsRed-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12070301
Proper citation: RRID:Addgene_12676 Copy
Species: Homo sapiens
Genetic Insert: RAB9 (Homo sapiens)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:p-DsRed-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12070301
Comments: This plasmid contains two tandem copies of the Rab9 gene.
Proper citation: RRID:Addgene_12677 Copy
Species: S. pyogenes
Genetic Insert: SpCas9-HF1
Vector Backbone Description: Vector Backbone:pMJ806-like; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32144253
Comments: pET-T7-6xHis-MBP-TEV-FLAG-NLS-SpCas9-HF1-NLS
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126770 Copy
Species: S. pyogenes
Genetic Insert: eSpCas9-plus
Vector Backbone Description: Vector Backbone:pMJ806-like; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32144253
Comments: pET-T7-6xHis-MBP-TEV-FLAG-NLS-eSpCas9-plus-NLS
eSpCas9-plus cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs. Shows almost the same fidelity and target selectivity as its parental increased fidelity variant, eSpCas9.
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126774 Copy
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