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Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5520; Vector Backbone:pRS40x; Vector Types:Yeast integrating vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: ADE2 has been modified to remove HindIII and XbaI sites without altering the amino acid sequence of the Ade2 protein.
Proper citation: RRID:Addgene_35434 Copy
Species: Synthetic
Genetic Insert: TALE-Oct4
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21248753
Proper citation: RRID:Addgene_35391 Copy
Species: Mus musculus
Genetic Insert: Myc
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22340591
Proper citation: RRID:Addgene_35395 Copy
Species: Mus musculus
Genetic Insert: Mycn
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22340591
Proper citation: RRID:Addgene_35394 Copy
Species: Synthetic
Genetic Insert: TALE-Sox2
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21248753
Proper citation: RRID:Addgene_35388 Copy
Species: Homo sapiens
Genetic Insert: Protein Kinase C iota
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:11076; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the vector backbone, pWPI, is a bicistronic vector that allows for simultaneous expression of a transgene (PRKCI in this case) and an EGFP marker to facilitate tracking of transduced cells. The EGFP marker cDNA has been inserted downstream of EMCV IRES.
The PRKCI insert in this plasmid is a known variant that lack the first nine amino acids of PRKCI when compared to GenBank entry NP_002731.4
Proper citation: RRID:Addgene_35387 Copy
Species: Homo sapiens
Genetic Insert: hPLK2
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11437; Vector Backbone:pWPI/Neomycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21849493
Comments: The backbone of this bicistronic lentiviral vector was originally created by Dr. Didier Trono lab (pWPI, Plasmid # 12254), and then modified by Robert Strome using Neomycin to replace EGFP and adding several multiple clone sites. The MCS includes Swa I, Nde I, BamH I, Spe I, Xma I and Sma I (see sequence). The hPlk2WT cDNA was inserted with BamH I site, and can be replaced by any other gene with this site. This vector allows the simultaneous expression of hPlk2WT and Neomycin to facilitate establishing stable mammalian transgene cell lines.
Please note that the full sequence for this plasmid is approximated and not fully verified. For further cloning strategies, please contact with Dr. Anurag Tandon.
Proper citation: RRID:Addgene_35385 Copy
Species: Rattus norvegicus
Genetic Insert: βarrestin2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15699045
Proper citation: RRID:Addgene_35411 Copy
Species: Homo sapiens
Genetic Insert: PSF
Vector Backbone Description: Backbone Size:3800; Vector Backbone:pCMVmyc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16024807
Proper citation: RRID:Addgene_35377 Copy
Species: Homo sapiens
Genetic Insert: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2997; Vector Backbone:pGEM7Zf(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9214616
Proper citation: RRID:Addgene_35416 Copy
Species: Rattus norvegicus
Genetic Insert: beta-arrestin-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21857681
Proper citation: RRID:Addgene_35401 Copy
Species: Homo sapiens
Genetic Insert: DGK beta
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17272726
Proper citation: RRID:Addgene_35405 Copy
Species: Bos taurus
Genetic Insert: GRK2
Vector Backbone Description: Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7629102
Proper citation: RRID:Addgene_35403 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:4191; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000118; Origin: BBR1
Proper citation: RRID:Addgene_35352 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:4186; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000096; Origin: SC101
Proper citation: RRID:Addgene_35330 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-2
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:3700; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_178898 Copy
Species: X. albilineans
Genetic Insert: X. albilineans type I-F1 cas2-3
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178769 Copy
Species: Homo sapiens
Genetic Insert: human rhinovirus 3C protease
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178885 Copy
Species: X. albilineans
Genetic Insert: X. albilineans type I-C cas3
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178766 Copy
Species: Caenorhabditis elegans
Genetic Insert: EGL-3
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7538; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_178791 Copy
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