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Species: Homo sapiens
Genetic Insert: EDF1 endothelial differentiation-related factor 1
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38079 Copy
Species: Homo sapiens
Genetic Insert: API5 apoptosis inhibitor 5
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38074 Copy
Species: Homo sapiens
Genetic Insert: KIAA1967
Vector Backbone Description: Backbone Size:5539; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38082 Copy
Species: Homo sapiens
Genetic Insert: MYBBP1A MYB binding protein (P160) 1a
Vector Backbone Description: Backbone Size:5499; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38084 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BVLF1.5
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37949 Copy
Species: Homo sapiens
Genetic Insert: HNRNPR heterogeneous nuclear ribonucleoprotein R
Vector Backbone Description: Backbone Size:5538; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38067 Copy
Species: Homo sapiens
Genetic Insert: HNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38068 Copy
Species: Homo sapiens
Genetic Insert: MYEF2 myelin expression factor 2
Vector Backbone Description: Backbone Size:5508; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Comments: Digest and NGS results suggest that the original Gateway entry vector is still present in the DNA stock from which the bacterial stocks are created. Because the entry vector does not contain a promoter for the insert or selection marker, it should not interfere with function.
To attempt to remove the entry vector, recipient scientists could try to cure the plasmid from isolated colonies. Alternatively, they can linearize the entry vector with BmtI, HpaI or NheI digest, which will not cut the expression vector, and retransform into bacteria.
Proper citation: RRID:Addgene_38069 Copy
Species: Human adenovirus 5
Genetic Insert: E4 orf1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, adding several amino acid residues [STFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_38063 Copy
Species: Homo sapiens
Genetic Insert: HNRNPD heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38066 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BILF1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37938 Copy
Genetic Insert: tTA
Vector Backbone Description: Vector Backbone:pXCXCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15580589
Comments: Adenoviral plasmid for overexpression of tet transactivator (tTA) from CMV promoter with WPRE.
Additional reference information for this plasmid can be found at: https://www.ncbi.nlm.nih.gov/pubmed/15542617
Proper citation: RRID:Addgene_38057 Copy
Genetic Insert: tTA
Vector Backbone Description: Vector Backbone:pXCXCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15580589
Comments: Adenoviral plasmid for overexpression of tet transactivator (tTA) from Synapsin promoter with WPRE.
Additional reference information for this plasmid can be found at: https://www.ncbi.nlm.nih.gov/pubmed/15542617
Proper citation: RRID:Addgene_38059 Copy
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pXCXCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11991741
Comments: Adenoviral plasmid for overexpression of EGFP from synapsin promoter
Proper citation: RRID:Addgene_38054 Copy
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pXCXCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15580589
Comments: Adenoviral plasmid for overexpression of EGFP from tetracycline responsive element (TRE), intended to be used in conjucntion with one of the plasmids expressing tet transactivator (tTA), such as Addgene plasmid #'s 38056, 38057, 38058 or 38059.
Additional reference information for this plasmid can be found at: https://www.ncbi.nlm.nih.gov/pubmed/15542617
Proper citation: RRID:Addgene_38060 Copy
Species: Human adenovirus 5
Genetic Insert: E1B 19K
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, adding several amino acid residues [STFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_38047 Copy
Vector Backbone Description: Backbone Marker:Masatoshi Takeichi; Backbone Size:4839; Vector Backbone:CA-MCS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681690
Proper citation: RRID:Addgene_38041 Copy
Species: Synthetic
Genetic Insert: Avian sarcoma and leukemia virus receptor 950
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5604; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681690
Proper citation: RRID:Addgene_38044 Copy
Species: Rattus norvegicus
Genetic Insert: CREB
Vector Backbone Description: Vector Backbone:pXCXCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15129168
Comments: Adenoviral plasmid for overexpression of CREB from CMV promoter
Proper citation: RRID:Addgene_38050 Copy
Species: Homo sapiens
Genetic Insert: BCR/ABL P190
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLEF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18070886
Comments: Constructed by Suparna Mishra. Generated in a 3-way ligation consisting of 5' 0.75 kb BamHI/Sal I + 3' 6.3 kb Sal I/EcoRI BCR/ABL fragments into pLEF digested with BamHIxEcoRI. The ABL part of the insert contains the natural TAG and 3' UT sequences.
Addgene NGS identified a sequence discrepancy at nucleotide 3168 resulting in a T117M mutation in the ABL-1 sequence, relative to Genbank ID NP_005148.2 and Addgene Plasmid #38158. This is not a known polymorphism and the effect of this, if any, on the protein is not known.
Proper citation: RRID:Addgene_38159 Copy
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