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Species: Saccharomyces cerevisiae
Genetic Insert: Unstable ACT1 intron (WT)
Vector Backbone Description: Backbone Size:5731; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651636
Proper citation: RRID:Addgene_127608 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Unstable ECM33 intron (Mut)
Vector Backbone Description: Backbone Size:5731; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651636
Proper citation: RRID:Addgene_127607 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Stable ECM33 intron (WT)
Vector Backbone Description: Backbone Size:5731; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651636
Proper citation: RRID:Addgene_127606 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: APS3 intron
Vector Backbone Description: Backbone Size:4778; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651636
Proper citation: RRID:Addgene_127605 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ECM33 intron with MS2 hairpins inserted near 5′ end of intron
Vector Backbone Description: Backbone Size:5876; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651636
Proper citation: RRID:Addgene_127599 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: his-Hsp82
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7929182
Proper citation: RRID:Addgene_1277 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SPE1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3716; Vector Backbone:pET-20b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31367038
Proper citation: RRID:Addgene_117145 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1-Sup35 fusion
Vector Backbone Description: Backbone Size:6018; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10678178
Comments: *Note, Addgene's quality control sequencing finds S263P and N392T amino acid residue substitutions in the Rnq1 fragment. The effect of these residue changes is unknown.
Proper citation: RRID:Addgene_1173 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1-Sup35 fusion
Vector Backbone Description: Backbone Marker:Hieter and Sikorski; Backbone Size:4895; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10678178
Proper citation: RRID:Addgene_1174 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1-Sup35 fusion
Vector Backbone Description: Backbone Size:6018; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10678178
Proper citation: RRID:Addgene_1175 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RNQ1
Vector Backbone Description: Backbone Marker:Gibco-BRL; Backbone Size:5000; Vector Backbone:pPROEX-HTb; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10678178
Proper citation: RRID:Addgene_1176 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Brainbow 1.0 L
Vector Backbone Description: Vector Backbone:pT2-Ip2GI; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23757414
Proper citation: RRID:Addgene_118216 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc20/AID(71-114)-tCYC1-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31606371
Comments: This plasmid can be used to dynamically and conditionally deplete Cdc20 in yeast.
The work is currently under review.
Proper citation: RRID:Addgene_130269 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 F130W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1302 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 L553W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1306 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y507W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1305 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y819W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1307 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 F130W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1309 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FlpO-2A-Cre
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:pOG44; Vector Types:Mammalian Expression, Cre/Lox, Unspecified, episomal expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31539496
Comments: Self Excising- FlpO and Cre are surrounded by F5 FRT sites but the FlpO contains an intron to prevent bacterial self-recombination during DNA production
Proper citation: RRID:Addgene_130986 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScExo84
Vector Backbone Description: Backbone Marker:Sikorski and Hieter, 1991; Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21938623
Proper citation: RRID:Addgene_131165 Copy
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