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Species: S. pyogenes
Genetic Insert: B-eSpCas9
Vector Backbone Description: Vector Backbone:pMJ806-like; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32144253
Comments: pET-T7-6xHis-MBP-TEV-FLAG-NLS-Blackjack-eSpCas9-NLS
Blackjack variants cleave both 20nt and 5'-extended 21nt spacer containing sgRNAs. B-eSpCas9 shows higher fidelity and target selectivity than its parental increased fidelity variant, eSpCas9.
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126772 Copy
Species: S. pyogenes
Genetic Insert: SpCas9-HF1-plus
Vector Backbone Description: Vector Backbone:pMJ806-like; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32144253
Comments: pET-T7-6xHis-MBP-TEV-FLAG-NLS-SpCas9-HF1-plus-NLS
SpCas9-HF1-plus cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs. Shows almost the same fidelity and target selectivity as its parental increased fidelity variant, SpCas9-HF1.
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126775 Copy
Species: S. pyogenes
Genetic Insert: sgRNA (for SpCas9)
Vector Backbone Description: Vector Backbone:pmCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32144253
Comments: gRNA cloning vector (U6_BbsI sites_sgRNA) with mCherry transfection control (CMV_mCherry cassette), lacks the direct repeat (a truncated guideRNA) after the 3'-end of the sgRNA.
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126776 Copy
Species: Homo sapiens
Genetic Insert: Human Cdc45
Vector Backbone Description: Backbone Size:3766; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27189187
Proper citation: RRID:Addgene_126879 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter.
Proper citation: RRID:Addgene_126904 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter.
Proper citation: RRID:Addgene_126902 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter.
Proper citation: RRID:Addgene_126900 Copy
Vector Backbone Description: Backbone Marker:Backbone derived from SEVA.; Vector Backbone:pJUMP29-1A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33623824
Comments: Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_126960 Copy
Vector Backbone Description: Backbone Marker:Backbone derived from SEVA.; Vector Backbone:pJUMP29-1B; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33623824
Comments: Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_126961 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter; encodes the plant selectable marker nptII driven by the rice Act1 promoter.
Proper citation: RRID:Addgene_126890 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter.
Proper citation: RRID:Addgene_126895 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter.
Proper citation: RRID:Addgene_126897 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter; encodes the plant selectable marker nptII driven by the rice Act1 promoter.
Proper citation: RRID:Addgene_126883 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter; encodes the plant selectable marker nptII driven by the rice Act1 promoter.
Proper citation: RRID:Addgene_126887 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter; encodes the plant selectable marker nptII driven by the rice Act1 promoter.
Proper citation: RRID:Addgene_126885 Copy
Vector Backbone Description: Backbone Marker:Backbone derived from SEVA.; Vector Backbone:pJUMP25-1A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33623824
Comments: Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_126972 Copy
Vector Backbone Description: Backbone Marker:Backbone derived from SEVA.; Vector Backbone:pJUMP23-1A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33623824
Comments: Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_126970 Copy
Vector Backbone Description: Backbone Marker:Backbone derived from SEVA.; Vector Backbone:pJUMP28-1A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33623824
Comments: Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_126976 Copy
Vector Backbone Description: Backbone Marker:Backbone derived from SEVA.; Vector Backbone:pJUMP26-1A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33623824
Comments: Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_126973 Copy
Vector Backbone Description: Backbone Marker:Backbone derived from SEVA.; Vector Backbone:pJUMP27ts-1A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33623824
Comments: Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_126977 Copy
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