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Species: Homo sapiens
Genetic Insert: c-MYC
Vector Backbone Description: Backbone Marker:Dr. Robert Hawley of George Washington University; Backbone Size:5660; Vector Backbone:MSCV IRES GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Comments: The MSCV vector is described in; Hawley, R.G., Lieu, F.H.L., Fong, A.Z.C., and Hawley, T.S. Versatile retroviral vectors for potential use in gene therapy. Gene Ther. 1: 136-138, 1994.
Use of the construct is described in; Park, I., Zhao, R., West, J.A., Yabuuchi, A., Huo, H., Ince, T.A., Lerou, P.H., Lensch, M.W., and Daley, G.Q. Reprogramming of human somatic cells to pluripotency with defined factors. Nature, 451, 141-146, 2008.
Please note that the assembled sequence presented here is not 100% reflective of the actual sequence. See the Addgene diagnostic digest (found under the Resource Information section of the plasmid page) for additional information on the expected plasmid size. Addgene has found another HindIII site at the end of the MYC gene (see our quality control sequence).
Proper citation: RRID:Addgene_18119 Copy
Species: Homo sapiens
Genetic Insert: PD-1
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34734802
Proper citation: RRID:Addgene_180819 Copy
Species: Homo sapiens
Genetic Insert: MMLVgag-3xNES-ABE8e
Vector Backbone Description: Backbone Size:4757; Vector Backbone:pCDNA3-like; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35021064
Comments: the backbone was amplified from BIC-Gag-CAS9
(Plasmid #119942)
Proper citation: RRID:Addgene_181751 Copy
Species: Homo sapiens
Genetic Insert: CD82
Vector Backbone Description: Backbone Marker:BD Biosciences / Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14617360
Proper citation: RRID:Addgene_1818 Copy
Species: Homo sapiens
Genetic Insert: CD82
Vector Backbone Description: Backbone Marker:BD Biosciences / Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14617360
Proper citation: RRID:Addgene_1819 Copy
Species: Synthetic
Genetic Insert: bacterial codon optimized SpRY
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36203014
Proper citation: RRID:Addgene_181743 Copy
Species: Synthetic
Genetic Insert: human/zebrafish codon optimized SpCas9
Vector Backbone Description: Vector Backbone:BPK764 (Addgene Plasmid #65767); Vector Types:Bacterial Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36203014
Proper citation: RRID:Addgene_181745 Copy
Species: S. pyogenes
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Proper citation: RRID:Addgene_181906 Copy
Species: Mus musculus
Genetic Insert: MECP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32101700
Proper citation: RRID:Addgene_181904 Copy
Genetic Insert: MCP-M-MLV-RT
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35379962
Proper citation: RRID:Addgene_181799 Copy
Species: Synthetic
Genetic Insert: Rep2 and Ref2
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35281741
Proper citation: RRID:Addgene_182055 Copy
Species: Synthetic
Genetic Insert: Rep1 and Ref1
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35281741
Proper citation: RRID:Addgene_182054 Copy
Species: Synthetic
Genetic Insert: Rep6 and Ref6
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35281741
Proper citation: RRID:Addgene_182059 Copy
Species: Homo sapiens
Genetic Insert: RUNX1C (human)
Vector Backbone Description: Vector Backbone:pRRL.PPT.SFFV.MCS.IRES.dTomato; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36493345
Proper citation: RRID:Addgene_181980 Copy
Species: Vesicular Stomatitis Virus
Genetic Insert: VSVGmut
Vector Backbone Description: Backbone Size:4284; Vector Backbone:pMD2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35396484
Comments: Mutations originally described in Nikolic et al 2018: https://www.nature.com/articles/s41467-018-03432-4.
Proper citation: RRID:Addgene_182229 Copy
Species: Synthetic
Genetic Insert: cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36303019
Comments: Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
Proper citation: RRID:Addgene_182282 Copy
Species: Synthetic
Genetic Insert: cAMPFIRE-M (cAMP sensor)
Vector Backbone Description: Backbone Size:5922; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36303019
Comments: Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
Proper citation: RRID:Addgene_182284 Copy
Species: Synthetic
Genetic Insert: cAMPFIRE-L (cAMP sensor)
Vector Backbone Description: Backbone Size:5922; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36303019
Comments: Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
Proper citation: RRID:Addgene_182283 Copy
Species: Homo sapiens
Genetic Insert: MYOD1-shOct4
Vector Backbone Description: Backbone Size:13225; Vector Backbone:pUCM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182309 Copy
Species: Brevibacillus sp. SYP-B805
Genetic Insert: BrCas12b
Vector Backbone Description: Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35290826
Proper citation: RRID:Addgene_182276 Copy
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