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Species: Mus musculus
Genetic Insert: Slc17a7-IRES2-Cre
Vector Backbone Description: Backbone Size:2657; Vector Backbone:minimal cloning vector; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25741722
Comments: The Zeng lab recommends amplifying this plasmid in 15 microg/ml kanamycin to reduce recombination. Addgene is not able to supply the plasmid under these selection conditions and the stab you receive will be under Amp selection. We recommend using the low conc. Kan selection and checking your plasmid prep for recombination.
Proper citation: RRID:Addgene_61574 Copy
Species: Mus musculus
Genetic Insert: Pvalb exon 4 - 2A - Cre
Vector Backbone Description: Backbone Size:4496; Vector Backbone:pBS SK2+; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25741722
Proper citation: RRID:Addgene_61570 Copy
Species: Homo sapiens
Genetic Insert: BRCA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: In frame stop codon between the HindIII site and ATG of BRCA1 cDNA.
Proper citation: RRID:Addgene_61586 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Lyn-AKAR4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20838685
Proper citation: RRID:Addgene_61620 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AKAR4-Kras
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20838685
Proper citation: RRID:Addgene_61621 Copy
Vector Backbone Description: Backbone Size:2959; Vector Backbone:pBS SK2+; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25741722
Comments: The Zeng lab recommends amplifying this plasmid in 15 microg/ml kanamycin to reduce recombination. Addgene is not able to supply the plasmid under these selection conditions and the stab you receive will be under Amp selection. We recommend using the low conc. Kan selection and checking your plasmid prep for recombination.
Proper citation: RRID:Addgene_61580 Copy
Species: Homo sapiens
Genetic Insert: rhoA
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7600581
Proper citation: RRID:Addgene_61632 Copy
Species: Homo sapiens
Genetic Insert: Rac1
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7600581
Proper citation: RRID:Addgene_61633 Copy
Species: Staphylococcus aureus
Genetic Insert: hSaCas9
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25830891
Proper citation: RRID:Addgene_61593 Copy
Species: Other
Genetic Insert: hSaCas9
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25830891
Comments: Note: This plasmid does not have an sgRNA expression cassette. Please refer to the associated publication, as well as the AAV portion of http://www.addgene.org/crispr/zhang/ , for more information on expressing sgRNAs.
Proper citation: RRID:Addgene_61594 Copy
Species: Synthetic
Genetic Insert: pHoran4
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25385186
Comments: There is a C terminal Myc tag in frame with pHoran.
Proper citation: RRID:Addgene_61557 Copy
Species: Synthetic
Genetic Insert: pHoran4
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pBAD/His-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25385186
Proper citation: RRID:Addgene_61558 Copy
Species: Mus musculus
Genetic Insert: Kdm4d
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25417163
Proper citation: RRID:Addgene_61553 Copy
Species: Synthetic
Genetic Insert: pHuji
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25385186
Comments: There is a N-terminal HA tag in frame with pHuji.
Proper citation: RRID:Addgene_61556 Copy
Species: Synthetic
Genetic Insert: HIV-1 integrase F185H/C280S
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pET-15b; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8631811
Proper citation: RRID:Addgene_61672 Copy
Species: horse
Genetic Insert: Latherin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET-32; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23782536
Proper citation: RRID:Addgene_61685 Copy
Species: Synthetic
Genetic Insert: HIV-1 integrase W235F
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pET-15b; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16482214
Proper citation: RRID:Addgene_61681 Copy
Species: Mus musculus
Genetic Insert: IFT88
Vector Backbone Description: Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23930224
Proper citation: RRID:Addgene_61684 Copy
Species: Homo sapiens
Genetic Insert: NAD(P)H dehydrogenase, quinone 1 (NQO1)
Vector Backbone Description: Vector Backbone:pEFIRES; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15687255
Comments: Addgene sequencing results find that the Y to V mutation is at position 129 with respect to reference sequence NP_000894.1
Proper citation: RRID:Addgene_61733 Copy
Species: Hepatitis C Virus
Genetic Insert: NS4A
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24130685
Comments: For protein expression, grow in Rosetta cells.
25 degree induction for 14-15 hours with 0.5 mM IPTG
Proper citation: RRID:Addgene_61696 Copy
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